Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1582 and Pf1N1B4_1583 are separated by 158 nucleotides Pf1N1B4_1583 and Pf1N1B4_1584 overlap by 4 nucleotides Pf1N1B4_1584 and Pf1N1B4_1585 are separated by 71 nucleotides
Pf1N1B4_1582 - Histidine ammonia-lyase (EC 4.3.1.3) <small>(from data)</small>, at 1,822,609 to 1,824,132
_1582
Pf1N1B4_1583 - Histidine ABC transporter, ATP-binding protein (TC 3.A.1), at 1,824,291 to 1,825,121
_1583
Pf1N1B4_1584 - Histidine ABC transporter, permease protein (TC 3.A.1), at 1,825,118 to 1,825,969
_1584
Pf1N1B4_1585 - Histidine ABC transporter, histidine-binding protein (TC 3.A.1), at 1,826,041 to 1,827,009
_1585
Position (kb) 1824
1825
1826 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1823.801 kb on + strand, within Pf1N1B4_1582 at 1823.801 kb on + strand, within Pf1N1B4_1582 at 1823.801 kb on + strand, within Pf1N1B4_1582 at 1823.801 kb on + strand, within Pf1N1B4_1582 at 1823.801 kb on + strand, within Pf1N1B4_1582 at 1823.801 kb on + strand, within Pf1N1B4_1582 at 1823.802 kb on - strand, within Pf1N1B4_1582 at 1823.802 kb on - strand, within Pf1N1B4_1582 at 1823.802 kb on - strand, within Pf1N1B4_1582 at 1823.802 kb on - strand, within Pf1N1B4_1582 at 1823.811 kb on + strand, within Pf1N1B4_1582 at 1823.964 kb on - strand, within Pf1N1B4_1582 at 1824.315 kb on + strand at 1824.315 kb on + strand at 1824.315 kb on + strand at 1824.316 kb on - strand at 1824.331 kb on + strand at 1824.331 kb on + strand at 1824.331 kb on + strand at 1824.331 kb on + strand at 1824.331 kb on + strand at 1824.332 kb on - strand at 1824.332 kb on - strand at 1824.332 kb on - strand at 1824.332 kb on - strand at 1824.332 kb on - strand at 1824.332 kb on - strand at 1824.629 kb on - strand, within Pf1N1B4_1583 at 1824.629 kb on - strand, within Pf1N1B4_1583 at 1824.629 kb on - strand, within Pf1N1B4_1583 at 1824.629 kb on - strand, within Pf1N1B4_1583 at 1824.629 kb on - strand, within Pf1N1B4_1583 at 1824.725 kb on - strand, within Pf1N1B4_1583 at 1824.725 kb on - strand, within Pf1N1B4_1583 at 1824.826 kb on + strand, within Pf1N1B4_1583 at 1824.826 kb on + strand, within Pf1N1B4_1583 at 1824.827 kb on - strand, within Pf1N1B4_1583 at 1824.827 kb on - strand, within Pf1N1B4_1583 at 1825.149 kb on + strand at 1825.149 kb on + strand at 1825.150 kb on - strand at 1825.159 kb on + strand at 1825.479 kb on + strand, within Pf1N1B4_1584 at 1825.480 kb on - strand, within Pf1N1B4_1584 at 1825.545 kb on + strand, within Pf1N1B4_1584 at 1825.896 kb on + strand at 1825.952 kb on - strand at 1825.969 kb on + strand at 1826.042 kb on + strand at 1826.042 kb on + strand at 1826.042 kb on + strand at 1826.043 kb on - strand at 1826.043 kb on - strand at 1826.043 kb on - strand at 1826.043 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 1,823,801 + Pf1N1B4_1582 0.78 +0.0 1,823,801 + Pf1N1B4_1582 0.78 +0.0 1,823,801 + Pf1N1B4_1582 0.78 +0.0 1,823,801 + Pf1N1B4_1582 0.78 -1.3 1,823,801 + Pf1N1B4_1582 0.78 -1.5 1,823,801 + Pf1N1B4_1582 0.78 +0.0 1,823,802 - Pf1N1B4_1582 0.78 +0.0 1,823,802 - Pf1N1B4_1582 0.78 +0.0 1,823,802 - Pf1N1B4_1582 0.78 +0.0 1,823,802 - Pf1N1B4_1582 0.78 -2.3 1,823,811 + Pf1N1B4_1582 0.79 +0.0 1,823,964 - Pf1N1B4_1582 0.89 +0.0 1,824,315 + +0.0 1,824,315 + +0.0 1,824,315 + -2.0 1,824,316 - -1.6 1,824,331 + +0.0 1,824,331 + +0.0 1,824,331 + +0.0 1,824,331 + +0.0 1,824,331 + +0.0 1,824,332 - +0.0 1,824,332 - +0.0 1,824,332 - -4.5 1,824,332 - +0.0 1,824,332 - -3.0 1,824,332 - +0.0 1,824,629 - Pf1N1B4_1583 0.41 +0.0 1,824,629 - Pf1N1B4_1583 0.41 +0.0 1,824,629 - Pf1N1B4_1583 0.41 -2.9 1,824,629 - Pf1N1B4_1583 0.41 +0.0 1,824,629 - Pf1N1B4_1583 0.41 +0.0 1,824,725 - Pf1N1B4_1583 0.52 +0.0 1,824,725 - Pf1N1B4_1583 0.52 +0.0 1,824,826 + Pf1N1B4_1583 0.64 +0.0 1,824,826 + Pf1N1B4_1583 0.64 +0.0 1,824,827 - Pf1N1B4_1583 0.65 +0.0 1,824,827 - Pf1N1B4_1583 0.65 -2.8 1,825,149 + +0.0 1,825,149 + -0.2 1,825,150 - +0.0 1,825,159 + +0.0 1,825,479 + Pf1N1B4_1584 0.42 -0.4 1,825,480 - Pf1N1B4_1584 0.42 -2.9 1,825,545 + Pf1N1B4_1584 0.50 +0.0 1,825,896 + +0.0 1,825,952 - -1.5 1,825,969 + +0.0 1,826,042 + +0.0 1,826,042 + +0.0 1,826,042 + -1.9 1,826,043 - +0.0 1,826,043 - -3.1 1,826,043 - +0.0 1,826,043 - -2.1
Or see this region's nucleotide sequence