Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_129 and Pf1N1B4_130 are separated by 0 nucleotides Pf1N1B4_130 and Pf1N1B4_131 are separated by 152 nucleotides Pf1N1B4_131 and Pf1N1B4_132 are separated by 326 nucleotides
Pf1N1B4_129 - sensory box histidine kinase/response regulator, at 140,909 to 144,151
_129
Pf1N1B4_130 - DNA-binding response regulator, LuxR family, at 144,152 to 144,772
_130
Pf1N1B4_131 - hypothetical protein, at 144,925 to 145,374
_131
Pf1N1B4_132 - L-ornithine 5-monooxygenase (EC 1.13.12.-), PvdA of pyoverdin biosynthesis, at 145,701 to 147,038
_132
Position (kb) 144
145
146 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 144.234 kb on + strand, within Pf1N1B4_130 at 144.234 kb on + strand, within Pf1N1B4_130 at 144.235 kb on - strand, within Pf1N1B4_130 at 144.235 kb on - strand, within Pf1N1B4_130 at 144.235 kb on - strand, within Pf1N1B4_130 at 144.265 kb on + strand, within Pf1N1B4_130 at 144.265 kb on + strand, within Pf1N1B4_130 at 144.266 kb on - strand, within Pf1N1B4_130 at 144.266 kb on - strand, within Pf1N1B4_130 at 144.266 kb on - strand, within Pf1N1B4_130 at 144.406 kb on - strand, within Pf1N1B4_130 at 144.406 kb on - strand, within Pf1N1B4_130 at 144.474 kb on + strand, within Pf1N1B4_130 at 144.474 kb on + strand, within Pf1N1B4_130 at 144.475 kb on - strand, within Pf1N1B4_130 at 144.475 kb on - strand, within Pf1N1B4_130 at 144.684 kb on + strand, within Pf1N1B4_130 at 144.684 kb on + strand, within Pf1N1B4_130 at 144.685 kb on - strand, within Pf1N1B4_130 at 144.776 kb on - strand at 144.776 kb on - strand at 144.899 kb on + strand at 145.121 kb on + strand, within Pf1N1B4_131 at 145.121 kb on + strand, within Pf1N1B4_131 at 145.121 kb on + strand at 145.121 kb on + strand, within Pf1N1B4_131 at 145.122 kb on - strand, within Pf1N1B4_131 at 145.122 kb on - strand, within Pf1N1B4_131 at 145.122 kb on - strand, within Pf1N1B4_131 at 145.233 kb on - strand, within Pf1N1B4_131 at 145.246 kb on - strand, within Pf1N1B4_131 at 145.353 kb on + strand at 145.447 kb on + strand at 145.475 kb on + strand at 145.475 kb on + strand at 145.476 kb on - strand at 145.476 kb on - strand at 145.486 kb on + strand at 145.487 kb on - strand at 145.487 kb on - strand at 145.487 kb on - strand at 145.487 kb on - strand at 145.487 kb on - strand at 145.702 kb on + strand at 145.703 kb on - strand at 145.768 kb on + strand at 145.768 kb on + strand at 145.769 kb on - strand at 145.861 kb on + strand, within Pf1N1B4_132 at 145.861 kb on + strand, within Pf1N1B4_132 at 145.861 kb on + strand, within Pf1N1B4_132 at 145.861 kb on + strand at 145.861 kb on + strand, within Pf1N1B4_132 at 145.861 kb on + strand, within Pf1N1B4_132 at 145.862 kb on - strand, within Pf1N1B4_132 at 145.862 kb on - strand, within Pf1N1B4_132 at 145.862 kb on - strand at 145.900 kb on + strand at 145.901 kb on - strand, within Pf1N1B4_132 at 145.931 kb on - strand, within Pf1N1B4_132 at 146.149 kb on + strand, within Pf1N1B4_132 at 146.149 kb on + strand, within Pf1N1B4_132 at 146.149 kb on + strand, within Pf1N1B4_132 at 146.149 kb on + strand, within Pf1N1B4_132 at 146.185 kb on + strand, within Pf1N1B4_132 at 146.186 kb on - strand, within Pf1N1B4_132 at 146.186 kb on - strand, within Pf1N1B4_132
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 144,234 + Pf1N1B4_130 0.13 -2.0 144,234 + Pf1N1B4_130 0.13 -0.0 144,235 - Pf1N1B4_130 0.13 -0.0 144,235 - Pf1N1B4_130 0.13 -1.9 144,235 - Pf1N1B4_130 0.13 -0.0 144,265 + Pf1N1B4_130 0.18 -0.9 144,265 + Pf1N1B4_130 0.18 -0.0 144,266 - Pf1N1B4_130 0.18 -0.0 144,266 - Pf1N1B4_130 0.18 -1.8 144,266 - Pf1N1B4_130 0.18 -0.0 144,406 - Pf1N1B4_130 0.41 -0.0 144,406 - Pf1N1B4_130 0.41 -0.0 144,474 + Pf1N1B4_130 0.52 -0.0 144,474 + Pf1N1B4_130 0.52 -0.0 144,475 - Pf1N1B4_130 0.52 -0.0 144,475 - Pf1N1B4_130 0.52 -0.0 144,684 + Pf1N1B4_130 0.86 -0.0 144,684 + Pf1N1B4_130 0.86 -0.0 144,685 - Pf1N1B4_130 0.86 -2.0 144,776 - -0.0 144,776 - -0.0 144,899 + -1.7 145,121 + Pf1N1B4_131 0.44 -0.0 145,121 + Pf1N1B4_131 0.44 -0.0 145,121 + -0.0 145,121 + Pf1N1B4_131 0.44 -3.0 145,122 - Pf1N1B4_131 0.44 -0.0 145,122 - Pf1N1B4_131 0.44 -0.0 145,122 - Pf1N1B4_131 0.44 -0.0 145,233 - Pf1N1B4_131 0.68 -0.0 145,246 - Pf1N1B4_131 0.71 -4.1 145,353 + -4.0 145,447 + -5.0 145,475 + -0.0 145,475 + -0.0 145,476 - -1.1 145,476 - -1.2 145,486 + -0.0 145,487 - -0.0 145,487 - -0.0 145,487 - -0.0 145,487 - -0.0 145,487 - -0.0 145,702 + -2.1 145,703 - -1.2 145,768 + -0.0 145,768 + -1.7 145,769 - -2.2 145,861 + Pf1N1B4_132 0.12 -1.6 145,861 + Pf1N1B4_132 0.12 -0.0 145,861 + Pf1N1B4_132 0.12 -0.0 145,861 + -0.0 145,861 + Pf1N1B4_132 0.12 -1.4 145,861 + Pf1N1B4_132 0.12 -2.8 145,862 - Pf1N1B4_132 0.12 -0.0 145,862 - Pf1N1B4_132 0.12 +0.6 145,862 - -0.7 145,900 + -0.0 145,901 - Pf1N1B4_132 0.15 -0.0 145,931 - Pf1N1B4_132 0.17 -0.0 146,149 + Pf1N1B4_132 0.33 -1.8 146,149 + Pf1N1B4_132 0.33 -0.0 146,149 + Pf1N1B4_132 0.33 -0.0 146,149 + Pf1N1B4_132 0.33 -0.0 146,185 + Pf1N1B4_132 0.36 -1.1 146,186 - Pf1N1B4_132 0.36 -0.0 146,186 - Pf1N1B4_132 0.36 -2.2
Or see this region's nucleotide sequence