Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_128

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_127 and Pf1N1B4_128 are separated by 254 nucleotidesPf1N1B4_128 and Pf1N1B4_129 are separated by 3 nucleotides Pf1N1B4_127 - FIG032225: Transcriptional regulator, LysR family, at 138,939 to 139,847 _127 Pf1N1B4_128 - Methylglyoxal reductase, acetol producing (EC 1.1.1.-) / 2,5-diketo-D-gluconic acid reductase B (EC 1.1.1.274), at 140,102 to 140,905 _128 Pf1N1B4_129 - sensory box histidine kinase/response regulator, at 140,909 to 144,151 _129 Position (kb) 140 141Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 139.134 kb on + strand, within Pf1N1B4_127at 139.134 kb on + strand, within Pf1N1B4_127at 139.281 kb on + strand, within Pf1N1B4_127at 139.281 kb on + strand, within Pf1N1B4_127at 139.282 kb on - strand, within Pf1N1B4_127at 139.282 kb on - strand, within Pf1N1B4_127at 139.282 kb on - strand, within Pf1N1B4_127at 139.282 kb on - strand, within Pf1N1B4_127at 139.293 kb on + strand, within Pf1N1B4_127at 139.293 kb on + strand, within Pf1N1B4_127at 139.294 kb on - strand, within Pf1N1B4_127at 139.498 kb on + strand, within Pf1N1B4_127at 139.542 kb on + strand, within Pf1N1B4_127at 140.007 kb on + strandat 140.063 kb on + strandat 140.129 kb on - strandat 140.521 kb on + strand, within Pf1N1B4_128at 140.522 kb on - strand, within Pf1N1B4_128at 140.574 kb on - strand, within Pf1N1B4_128at 140.634 kb on - strand, within Pf1N1B4_128at 140.634 kb on - strand, within Pf1N1B4_128at 140.634 kb on - strand, within Pf1N1B4_128at 140.634 kb on - strand, within Pf1N1B4_128at 140.956 kb on + strandat 140.956 kb on + strandat 140.956 kb on + strandat 140.956 kb on + strandat 140.956 kb on + strandat 140.957 kb on - strandat 140.957 kb on - strandat 140.957 kb on - strandat 141.353 kb on - strand, within Pf1N1B4_129at 141.353 kb on - strand, within Pf1N1B4_129at 141.490 kb on + strand, within Pf1N1B4_129

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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139,134 + Pf1N1B4_127 0.21 -2.7
139,134 + Pf1N1B4_127 0.21 -0.0
139,281 + Pf1N1B4_127 0.38 -0.0
139,281 + Pf1N1B4_127 0.38 -0.5
139,282 - Pf1N1B4_127 0.38 -0.8
139,282 - Pf1N1B4_127 0.38 -0.0
139,282 - Pf1N1B4_127 0.38 -0.0
139,282 - Pf1N1B4_127 0.38 -0.0
139,293 + Pf1N1B4_127 0.39 -0.0
139,293 + Pf1N1B4_127 0.39 -1.1
139,294 - Pf1N1B4_127 0.39 -0.0
139,498 + Pf1N1B4_127 0.61 -0.0
139,542 + Pf1N1B4_127 0.66 -0.0
140,007 + -2.1
140,063 + -2.8
140,129 - -1.7
140,521 + Pf1N1B4_128 0.52 -2.6
140,522 - Pf1N1B4_128 0.52 -2.9
140,574 - Pf1N1B4_128 0.59 -4.1
140,634 - Pf1N1B4_128 0.66 -4.2
140,634 - Pf1N1B4_128 0.66 -0.0
140,634 - Pf1N1B4_128 0.66 -1.0
140,634 - Pf1N1B4_128 0.66 -0.0
140,956 + -2.5
140,956 + -0.0
140,956 + -1.2
140,956 + -0.0
140,956 + -0.0
140,957 - -1.5
140,957 - -3.2
140,957 - -2.2
141,353 - Pf1N1B4_129 0.14 +0.3
141,353 - Pf1N1B4_129 0.14 -0.0
141,490 + Pf1N1B4_129 0.18 -0.9

Or see this region's nucleotide sequence