Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_103 and Pf1N1B4_104 are separated by 493 nucleotides Pf1N1B4_104 and Pf1N1B4_105 overlap by 4 nucleotides Pf1N1B4_105 and Pf1N1B4_106 are separated by 3 nucleotides Pf1N1B4_106 and Pf1N1B4_107 are separated by 38 nucleotides
Pf1N1B4_103 - Histidine permease YuiF, at 108,555 to 109,871
_103
Pf1N1B4_104 - Sensor protein PhoQ (EC 2.7.13.3), at 110,365 to 111,678
_104
Pf1N1B4_105 - Two-component system response regulator QseB, at 111,675 to 112,343
_105
Pf1N1B4_106 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 112,347 to 112,628
_106
Pf1N1B4_107 - FIG00857858: hypothetical protein, at 112,667 to 112,975
_107
Position (kb) 110
111
112 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 109.399 kb on + strand, within Pf1N1B4_103 at 109.399 kb on + strand, within Pf1N1B4_103 at 109.399 kb on + strand, within Pf1N1B4_103 at 109.400 kb on - strand, within Pf1N1B4_103 at 109.471 kb on + strand, within Pf1N1B4_103 at 109.471 kb on + strand at 109.471 kb on + strand, within Pf1N1B4_103 at 109.472 kb on - strand at 109.472 kb on - strand, within Pf1N1B4_103 at 109.472 kb on - strand, within Pf1N1B4_103 at 109.473 kb on + strand, within Pf1N1B4_103 at 109.521 kb on + strand, within Pf1N1B4_103 at 109.522 kb on - strand, within Pf1N1B4_103 at 109.522 kb on - strand, within Pf1N1B4_103 at 109.834 kb on - strand at 109.861 kb on - strand at 109.942 kb on - strand at 109.942 kb on - strand at 109.976 kb on - strand at 109.976 kb on - strand at 109.978 kb on + strand at 110.134 kb on + strand at 110.135 kb on - strand at 110.135 kb on - strand at 110.135 kb on - strand at 110.238 kb on - strand at 110.443 kb on - strand at 110.715 kb on - strand, within Pf1N1B4_104 at 111.065 kb on - strand, within Pf1N1B4_104 at 111.249 kb on + strand, within Pf1N1B4_104 at 111.250 kb on - strand, within Pf1N1B4_104 at 111.251 kb on + strand at 111.251 kb on + strand, within Pf1N1B4_104 at 111.252 kb on - strand at 111.252 kb on - strand at 111.252 kb on - strand, within Pf1N1B4_104 at 111.252 kb on - strand, within Pf1N1B4_104 at 111.252 kb on - strand at 111.435 kb on + strand, within Pf1N1B4_104 at 111.436 kb on - strand, within Pf1N1B4_104 at 111.436 kb on - strand, within Pf1N1B4_104 at 111.436 kb on - strand, within Pf1N1B4_104 at 111.441 kb on - strand, within Pf1N1B4_104 at 111.441 kb on - strand, within Pf1N1B4_104 at 111.996 kb on + strand, within Pf1N1B4_105 at 112.232 kb on - strand, within Pf1N1B4_105 at 112.364 kb on + strand at 112.364 kb on + strand at 112.368 kb on + strand at 112.368 kb on + strand at 112.368 kb on + strand at 112.369 kb on - strand at 112.395 kb on + strand, within Pf1N1B4_106 at 112.409 kb on + strand, within Pf1N1B4_106 at 112.438 kb on + strand, within Pf1N1B4_106 at 112.438 kb on + strand at 112.438 kb on + strand, within Pf1N1B4_106 at 112.444 kb on + strand, within Pf1N1B4_106 at 112.668 kb on + strand at 112.668 kb on + strand at 112.668 kb on + strand at 112.668 kb on + strand at 112.668 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 109,399 + Pf1N1B4_103 0.64 -0.0 109,399 + Pf1N1B4_103 0.64 -0.0 109,399 + Pf1N1B4_103 0.64 -0.0 109,400 - Pf1N1B4_103 0.64 -0.0 109,471 + Pf1N1B4_103 0.70 -0.0 109,471 + -0.0 109,471 + Pf1N1B4_103 0.70 -0.0 109,472 - -0.0 109,472 - Pf1N1B4_103 0.70 +1.0 109,472 - Pf1N1B4_103 0.70 -0.0 109,473 + Pf1N1B4_103 0.70 -1.2 109,521 + Pf1N1B4_103 0.73 -0.0 109,522 - Pf1N1B4_103 0.73 -0.0 109,522 - Pf1N1B4_103 0.73 -0.0 109,834 - -0.0 109,861 - -0.0 109,942 - -0.0 109,942 - -0.0 109,976 - -3.0 109,976 - -4.2 109,978 + -0.0 110,134 + -0.0 110,135 - -0.0 110,135 - -0.0 110,135 - -0.0 110,238 - -0.0 110,443 - -0.0 110,715 - Pf1N1B4_104 0.27 -0.0 111,065 - Pf1N1B4_104 0.53 -2.0 111,249 + Pf1N1B4_104 0.67 -2.2 111,250 - Pf1N1B4_104 0.67 -0.0 111,251 + -0.0 111,251 + Pf1N1B4_104 0.67 -3.0 111,252 - -0.0 111,252 - -0.0 111,252 - Pf1N1B4_104 0.68 -4.3 111,252 - Pf1N1B4_104 0.68 -0.0 111,252 - -0.0 111,435 + Pf1N1B4_104 0.81 -1.8 111,436 - Pf1N1B4_104 0.82 -0.0 111,436 - Pf1N1B4_104 0.82 -4.1 111,436 - Pf1N1B4_104 0.82 -0.0 111,441 - Pf1N1B4_104 0.82 -0.0 111,441 - Pf1N1B4_104 0.82 -3.8 111,996 + Pf1N1B4_105 0.48 -2.9 112,232 - Pf1N1B4_105 0.83 -0.0 112,364 + -0.0 112,364 + -0.0 112,368 + -0.0 112,368 + -0.0 112,368 + -2.6 112,369 - -0.0 112,395 + Pf1N1B4_106 0.17 -1.1 112,409 + Pf1N1B4_106 0.22 -0.0 112,438 + Pf1N1B4_106 0.32 -0.0 112,438 + -1.6 112,438 + Pf1N1B4_106 0.32 -4.2 112,444 + Pf1N1B4_106 0.34 -5.0 112,668 + -0.0 112,668 + -2.7 112,668 + -0.0 112,668 + -1.6 112,668 + -0.0
Or see this region's nucleotide sequence