Experiment: H2 N-free with 10mM L-Methionine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08210 and MMP_RS08215 are separated by 82 nucleotides MMP_RS08215 and MMP_RS08220 are separated by 15 nucleotides MMP_RS08220 and MMP_RS08225 are separated by 30 nucleotides MMP_RS08225 and MMP_RS08230 are separated by 27 nucleotides
MMP_RS08210: MMP_RS08210 - phosphatidylglycerophosphatase A, at 1,546,157 to 1,546,645
_RS08210
MMP_RS08215: MMP_RS08215 - DUF483 domain-containing protein, at 1,546,728 to 1,547,282
_RS08215
MMP_RS08220: MMP_RS08220 - TrkA family potassium uptake protein, at 1,547,298 to 1,547,963
_RS08220
MMP_RS08225: MMP_RS08225 - RimK family alpha-L-glutamate ligase, at 1,547,994 to 1,548,800
_RS08225
MMP_RS08230: MMP_RS08230 - 50S ribosomal protein L11 methyltransferase, at 1,548,828 to 1,549,610
_RS08230
Position (kb)
1547
1548 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1546.508 kb on + strand, within MMP_RS08210 at 1546.590 kb on - strand, within MMP_RS08210 at 1546.651 kb on - strand at 1546.685 kb on + strand at 1546.693 kb on - strand at 1546.694 kb on + strand at 1546.695 kb on - strand at 1546.696 kb on + strand at 1546.696 kb on + strand at 1546.746 kb on - strand at 1546.840 kb on + strand, within MMP_RS08215 at 1547.033 kb on + strand, within MMP_RS08215 at 1547.033 kb on + strand, within MMP_RS08215 at 1547.064 kb on + strand, within MMP_RS08215 at 1547.374 kb on - strand at 1547.500 kb on - strand at 1547.501 kb on + strand at 1547.996 kb on + strand at 1548.000 kb on - strand at 1548.009 kb on + strand at 1548.017 kb on + strand at 1548.062 kb on - strand at 1548.088 kb on + strand, within MMP_RS08225 at 1548.310 kb on - strand, within MMP_RS08225 at 1548.311 kb on + strand, within MMP_RS08225 at 1548.311 kb on + strand, within MMP_RS08225 at 1548.418 kb on - strand, within MMP_RS08225 at 1548.418 kb on - strand, within MMP_RS08225 at 1548.418 kb on - strand, within MMP_RS08225 at 1548.418 kb on - strand, within MMP_RS08225 at 1548.419 kb on + strand, within MMP_RS08225 at 1548.419 kb on + strand, within MMP_RS08225 at 1548.419 kb on + strand, within MMP_RS08225 at 1548.419 kb on + strand, within MMP_RS08225 at 1548.420 kb on - strand, within MMP_RS08225 at 1548.420 kb on - strand, within MMP_RS08225 at 1548.430 kb on + strand, within MMP_RS08225 at 1548.532 kb on + strand, within MMP_RS08225
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Methionine remove 1,546,508 + MMP_RS08210 0.72 -2.7 1,546,590 - MMP_RS08210 0.89 -3.1 1,546,651 - -0.8 1,546,685 + -0.7 1,546,693 - -2.8 1,546,694 + -0.6 1,546,695 - -4.8 1,546,696 + -2.2 1,546,696 + -2.1 1,546,746 - -2.9 1,546,840 + MMP_RS08215 0.20 -0.6 1,547,033 + MMP_RS08215 0.55 -1.7 1,547,033 + MMP_RS08215 0.55 -1.3 1,547,064 + MMP_RS08215 0.61 +0.2 1,547,374 - -2.2 1,547,500 - -2.8 1,547,501 + -1.4 1,547,996 + +1.4 1,548,000 - +0.8 1,548,009 + -1.4 1,548,017 + -2.5 1,548,062 - -1.3 1,548,088 + MMP_RS08225 0.12 -2.8 1,548,310 - MMP_RS08225 0.39 -0.2 1,548,311 + MMP_RS08225 0.39 -0.2 1,548,311 + MMP_RS08225 0.39 -2.5 1,548,418 - MMP_RS08225 0.53 -2.3 1,548,418 - MMP_RS08225 0.53 -2.0 1,548,418 - MMP_RS08225 0.53 -2.4 1,548,418 - MMP_RS08225 0.53 -1.8 1,548,419 + MMP_RS08225 0.53 -2.7 1,548,419 + MMP_RS08225 0.53 -1.3 1,548,419 + MMP_RS08225 0.53 -1.8 1,548,419 + MMP_RS08225 0.53 -1.2 1,548,420 - MMP_RS08225 0.53 -1.4 1,548,420 - MMP_RS08225 0.53 -0.2 1,548,430 + MMP_RS08225 0.54 -1.1 1,548,532 + MMP_RS08225 0.67 -1.4
Or see this region's nucleotide sequence