Experiment: H2 N-free with 10mM L-Methionine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03045 and MMP_RS03050 are separated by 99 nucleotides MMP_RS03050 and MMP_RS03055 are separated by 9 nucleotides MMP_RS03055 and MMP_RS03060 are separated by 94 nucleotides
MMP_RS03045: MMP_RS03045 - chorismate mutase, at 575,111 to 575,401
_RS03045
MMP_RS03050: MMP_RS03050 - DUF115 domain-containing protein, at 575,501 to 576,217
_RS03050
MMP_RS03055: MMP_RS03055 - anaerobic ribonucleoside-triphosphate reductase activating protein, at 576,227 to 576,937
_RS03055
MMP_RS03060: MMP_RS03060 - MBL fold metallo-hydrolase, at 577,032 to 577,814
_RS03060
Position (kb) 576
577 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 575.333 kb on - strand at 575.402 kb on + strand at 575.402 kb on + strand at 575.451 kb on - strand at 575.490 kb on + strand at 575.644 kb on + strand at 575.737 kb on + strand at 576.093 kb on + strand at 576.455 kb on + strand at 576.558 kb on - strand at 576.964 kb on - strand at 576.983 kb on - strand at 576.984 kb on + strand at 576.991 kb on + strand at 576.992 kb on - strand at 576.993 kb on + strand at 576.993 kb on + strand at 577.007 kb on - strand at 577.105 kb on - strand at 577.324 kb on + strand, within MMP_RS03060 at 577.358 kb on - strand, within MMP_RS03060 at 577.446 kb on + strand, within MMP_RS03060 at 577.520 kb on + strand, within MMP_RS03060 at 577.551 kb on + strand, within MMP_RS03060 at 577.557 kb on + strand, within MMP_RS03060 at 577.587 kb on + strand, within MMP_RS03060 at 577.595 kb on + strand, within MMP_RS03060 at 577.841 kb on + strand at 577.845 kb on - strand at 577.845 kb on - strand at 577.848 kb on + strand at 577.880 kb on + strand at 577.880 kb on + strand at 577.881 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Methionine remove 575,333 - -2.6 575,402 + -1.9 575,402 + -2.3 575,451 - -2.3 575,490 + -1.5 575,644 + -2.1 575,737 + -4.4 576,093 + -2.1 576,455 + -1.7 576,558 - -2.3 576,964 - -1.5 576,983 - -0.4 576,984 + -2.9 576,991 + -0.1 576,992 - +0.2 576,993 + -0.4 576,993 + -1.1 577,007 - -0.2 577,105 - +0.6 577,324 + MMP_RS03060 0.37 -0.3 577,358 - MMP_RS03060 0.42 -0.2 577,446 + MMP_RS03060 0.53 +0.2 577,520 + MMP_RS03060 0.62 +0.3 577,551 + MMP_RS03060 0.66 -0.8 577,557 + MMP_RS03060 0.67 -2.7 577,587 + MMP_RS03060 0.71 -1.9 577,595 + MMP_RS03060 0.72 +0.4 577,841 + +0.4 577,845 - +0.1 577,845 - +1.1 577,848 + +0.3 577,880 + -0.1 577,880 + +1.4 577,881 - -0.9
Or see this region's nucleotide sequence