Experiment: H2 N-free with 10mM L-Methionine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01450 and MMP_RS01455 are separated by 245 nucleotides MMP_RS01455 and MMP_RS01460 are separated by 23 nucleotides MMP_RS01460 and MMP_RS01465 are separated by 1 nucleotides
MMP_RS01450: MMP_RS01450 - tRNA pseudouridine(38-40) synthase TruA, at 267,810 to 268,598
_RS01450
MMP_RS01455: MMP_RS01455 - nickel pincer cofactor biosynthesis protein LarC, at 268,844 to 270,058
_RS01455
MMP_RS01460: MMP_RS01460 - hypothetical protein, at 270,082 to 270,741
_RS01460
MMP_RS01465: MMP_RS01465 - hypothetical protein, at 270,743 to 271,441
_RS01465
Position (kb) 268
269
270
271 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 267.904 kb on - strand, within MMP_RS01450 at 267.904 kb on - strand, within MMP_RS01450 at 267.904 kb on - strand, within MMP_RS01450 at 267.905 kb on + strand, within MMP_RS01450 at 267.905 kb on + strand, within MMP_RS01450 at 267.926 kb on - strand, within MMP_RS01450 at 267.930 kb on + strand, within MMP_RS01450 at 267.988 kb on - strand, within MMP_RS01450 at 268.037 kb on - strand, within MMP_RS01450 at 268.107 kb on - strand, within MMP_RS01450 at 268.279 kb on + strand, within MMP_RS01450 at 268.283 kb on - strand, within MMP_RS01450 at 268.283 kb on - strand, within MMP_RS01450 at 268.283 kb on - strand, within MMP_RS01450 at 268.283 kb on - strand, within MMP_RS01450 at 268.284 kb on + strand, within MMP_RS01450 at 268.284 kb on + strand, within MMP_RS01450 at 268.284 kb on + strand, within MMP_RS01450 at 268.413 kb on - strand, within MMP_RS01450 at 268.418 kb on + strand, within MMP_RS01450 at 268.434 kb on - strand, within MMP_RS01450 at 268.587 kb on + strand at 268.587 kb on + strand at 268.590 kb on - strand at 268.590 kb on - strand at 268.594 kb on - strand at 268.594 kb on - strand at 268.602 kb on + strand at 268.602 kb on + strand at 268.610 kb on - strand at 268.612 kb on - strand at 268.612 kb on - strand at 268.612 kb on - strand at 268.612 kb on - strand at 268.613 kb on + strand at 268.657 kb on - strand at 268.657 kb on - strand at 268.657 kb on - strand at 268.660 kb on + strand at 268.694 kb on - strand at 268.694 kb on - strand at 268.701 kb on + strand at 268.703 kb on - strand at 268.704 kb on + strand at 269.100 kb on - strand, within MMP_RS01455 at 269.110 kb on - strand, within MMP_RS01455 at 269.155 kb on - strand, within MMP_RS01455 at 269.157 kb on - strand, within MMP_RS01455 at 269.209 kb on + strand, within MMP_RS01455 at 269.283 kb on + strand, within MMP_RS01455 at 269.701 kb on + strand, within MMP_RS01455 at 269.824 kb on - strand, within MMP_RS01455 at 270.061 kb on + strand at 270.061 kb on + strand at 270.064 kb on - strand at 270.069 kb on - strand at 270.069 kb on - strand at 270.070 kb on + strand at 270.070 kb on + strand at 270.071 kb on - strand at 270.071 kb on - strand at 270.072 kb on + strand at 270.075 kb on + strand at 270.078 kb on - strand at 270.079 kb on + strand at 270.084 kb on + strand at 270.088 kb on - strand at 270.224 kb on - strand, within MMP_RS01460 at 270.224 kb on - strand, within MMP_RS01460 at 270.324 kb on + strand, within MMP_RS01460 at 270.756 kb on - strand at 271.001 kb on - strand, within MMP_RS01465 at 271.023 kb on + strand, within MMP_RS01465 at 271.037 kb on + strand, within MMP_RS01465
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Methionine remove 267,904 - MMP_RS01450 0.12 -0.7 267,904 - MMP_RS01450 0.12 -1.8 267,904 - MMP_RS01450 0.12 +1.1 267,905 + MMP_RS01450 0.12 +0.3 267,905 + MMP_RS01450 0.12 -0.8 267,926 - MMP_RS01450 0.15 +0.1 267,930 + MMP_RS01450 0.15 -0.4 267,988 - MMP_RS01450 0.23 +0.3 268,037 - MMP_RS01450 0.29 +1.3 268,107 - MMP_RS01450 0.38 +0.9 268,279 + MMP_RS01450 0.59 -0.1 268,283 - MMP_RS01450 0.60 -0.4 268,283 - MMP_RS01450 0.60 +1.4 268,283 - MMP_RS01450 0.60 +1.1 268,283 - MMP_RS01450 0.60 +0.1 268,284 + MMP_RS01450 0.60 +0.5 268,284 + MMP_RS01450 0.60 +2.5 268,284 + MMP_RS01450 0.60 +0.3 268,413 - MMP_RS01450 0.76 -0.9 268,418 + MMP_RS01450 0.77 +1.2 268,434 - MMP_RS01450 0.79 +1.3 268,587 + -0.2 268,587 + -1.1 268,590 - +0.8 268,590 - +0.8 268,594 - +0.5 268,594 - +1.0 268,602 + -0.7 268,602 + +0.4 268,610 - +2.7 268,612 - +1.5 268,612 - +0.7 268,612 - +0.3 268,612 - +0.9 268,613 + +0.2 268,657 - +0.3 268,657 - -0.2 268,657 - -2.4 268,660 + +1.4 268,694 - +2.1 268,694 - +2.3 268,701 + -2.0 268,703 - -1.0 268,704 + +0.9 269,100 - MMP_RS01455 0.21 -3.6 269,110 - MMP_RS01455 0.22 +2.1 269,155 - MMP_RS01455 0.26 -3.7 269,157 - MMP_RS01455 0.26 -5.6 269,209 + MMP_RS01455 0.30 -2.8 269,283 + MMP_RS01455 0.36 -3.2 269,701 + MMP_RS01455 0.71 -1.6 269,824 - MMP_RS01455 0.81 -1.8 270,061 + +0.0 270,061 + +0.1 270,064 - -2.1 270,069 - +1.1 270,069 - +0.1 270,070 + +1.2 270,070 + -0.3 270,071 - +1.0 270,071 - +0.2 270,072 + +1.0 270,075 + +1.7 270,078 - +0.1 270,079 + -0.4 270,084 + -1.3 270,088 - +1.0 270,224 - MMP_RS01460 0.22 +0.2 270,224 - MMP_RS01460 0.22 +0.9 270,324 + MMP_RS01460 0.37 +0.6 270,756 - +0.9 271,001 - MMP_RS01465 0.37 +1.3 271,023 + MMP_RS01465 0.40 -2.8 271,037 + MMP_RS01465 0.42 +1.0
Or see this region's nucleotide sequence