Strain Fitness in Methanococcus maripaludis S2 around MMP_RS00670

Experiment: H2 N-free with 10mM L-Methionine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS00660 and MMP_RS00665 are separated by 9 nucleotidesMMP_RS00665 and MMP_RS00670 are separated by 33 nucleotidesMMP_RS00670 and MMP_RS00675 are separated by 168 nucleotidesMMP_RS00675 and MMP_RS00680 are separated by 132 nucleotides MMP_RS00660: MMP_RS00660 - alkaline phosphatase family protein, at 120,542 to 121,822 _RS00660 MMP_RS00665: MMP_RS00665 - hypothetical protein, at 121,832 to 122,641 _RS00665 MMP_RS00670: MMP_RS00670 - TIM barrel protein, at 122,675 to 123,331 _RS00670 MMP_RS00675: MMP_RS00675 - nitroreductase family protein, at 123,500 to 124,021 _RS00675 MMP_RS00680: MMP_RS00680 - N-acetyl-gamma-glutamyl-phosphate reductase, at 124,154 to 125,185 _RS00680 Position (kb) 122 123 124Strain fitness (log2 ratio) -1 0 1 2at 121.916 kb on + strand, within MMP_RS00665at 122.121 kb on - strand, within MMP_RS00665at 122.121 kb on - strand, within MMP_RS00665at 122.237 kb on - strand, within MMP_RS00665at 122.443 kb on - strand, within MMP_RS00665at 122.596 kb on - strandat 122.600 kb on - strandat 122.607 kb on + strandat 122.615 kb on + strandat 122.651 kb on - strandat 122.651 kb on - strandat 122.652 kb on + strandat 122.655 kb on - strandat 122.661 kb on + strandat 122.666 kb on - strandat 122.667 kb on + strandat 122.671 kb on - strandat 122.698 kb on - strandat 122.699 kb on + strandat 122.853 kb on - strand, within MMP_RS00670at 122.857 kb on - strand, within MMP_RS00670at 122.927 kb on - strand, within MMP_RS00670at 122.972 kb on - strand, within MMP_RS00670at 123.364 kb on - strandat 123.414 kb on - strandat 123.416 kb on - strandat 123.446 kb on - strandat 123.466 kb on - strandat 123.859 kb on + strand, within MMP_RS00675at 124.055 kb on - strandat 124.094 kb on + strandat 124.109 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Methionine
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121,916 + MMP_RS00665 0.10 +2.1
122,121 - MMP_RS00665 0.36 -0.6
122,121 - MMP_RS00665 0.36 +1.7
122,237 - MMP_RS00665 0.50 +1.3
122,443 - MMP_RS00665 0.75 +1.3
122,596 - -0.5
122,600 - +1.1
122,607 + -0.4
122,615 + +2.1
122,651 - -1.2
122,651 - -0.5
122,652 + +1.1
122,655 - +1.3
122,661 + +1.3
122,666 - +0.8
122,667 + +0.8
122,671 - +0.7
122,698 - +0.4
122,699 + +0.9
122,853 - MMP_RS00670 0.27 +0.3
122,857 - MMP_RS00670 0.28 -0.6
122,927 - MMP_RS00670 0.38 +0.6
122,972 - MMP_RS00670 0.45 +0.7
123,364 - +0.1
123,414 - -0.6
123,416 - -0.5
123,446 - +0.3
123,466 - +0.8
123,859 + MMP_RS00675 0.69 +1.7
124,055 - +0.9
124,094 + -1.0
124,109 + +0.3

Or see this region's nucleotide sequence