Experiment: H2 N-free with 10mM L-Methionine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00660 and MMP_RS00665 are separated by 9 nucleotides MMP_RS00665 and MMP_RS00670 are separated by 33 nucleotides MMP_RS00670 and MMP_RS00675 are separated by 168 nucleotides MMP_RS00675 and MMP_RS00680 are separated by 132 nucleotides
MMP_RS00660: MMP_RS00660 - alkaline phosphatase family protein, at 120,542 to 121,822
_RS00660
MMP_RS00665: MMP_RS00665 - hypothetical protein, at 121,832 to 122,641
_RS00665
MMP_RS00670: MMP_RS00670 - TIM barrel protein, at 122,675 to 123,331
_RS00670
MMP_RS00675: MMP_RS00675 - nitroreductase family protein, at 123,500 to 124,021
_RS00675
MMP_RS00680: MMP_RS00680 - N-acetyl-gamma-glutamyl-phosphate reductase, at 124,154 to 125,185
_RS00680
Position (kb)
122
123
124 Strain fitness (log2 ratio)
-1
0
1
2 at 121.916 kb on + strand, within MMP_RS00665 at 122.121 kb on - strand, within MMP_RS00665 at 122.121 kb on - strand, within MMP_RS00665 at 122.237 kb on - strand, within MMP_RS00665 at 122.443 kb on - strand, within MMP_RS00665 at 122.596 kb on - strand at 122.600 kb on - strand at 122.607 kb on + strand at 122.615 kb on + strand at 122.651 kb on - strand at 122.651 kb on - strand at 122.652 kb on + strand at 122.655 kb on - strand at 122.661 kb on + strand at 122.666 kb on - strand at 122.667 kb on + strand at 122.671 kb on - strand at 122.698 kb on - strand at 122.699 kb on + strand at 122.853 kb on - strand, within MMP_RS00670 at 122.857 kb on - strand, within MMP_RS00670 at 122.927 kb on - strand, within MMP_RS00670 at 122.972 kb on - strand, within MMP_RS00670 at 123.364 kb on - strand at 123.414 kb on - strand at 123.416 kb on - strand at 123.446 kb on - strand at 123.466 kb on - strand at 123.859 kb on + strand, within MMP_RS00675 at 124.055 kb on - strand at 124.094 kb on + strand at 124.109 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Methionine remove 121,916 + MMP_RS00665 0.10 +2.1 122,121 - MMP_RS00665 0.36 -0.6 122,121 - MMP_RS00665 0.36 +1.7 122,237 - MMP_RS00665 0.50 +1.3 122,443 - MMP_RS00665 0.75 +1.3 122,596 - -0.5 122,600 - +1.1 122,607 + -0.4 122,615 + +2.1 122,651 - -1.2 122,651 - -0.5 122,652 + +1.1 122,655 - +1.3 122,661 + +1.3 122,666 - +0.8 122,667 + +0.8 122,671 - +0.7 122,698 - +0.4 122,699 + +0.9 122,853 - MMP_RS00670 0.27 +0.3 122,857 - MMP_RS00670 0.28 -0.6 122,927 - MMP_RS00670 0.38 +0.6 122,972 - MMP_RS00670 0.45 +0.7 123,364 - +0.1 123,414 - -0.6 123,416 - -0.5 123,446 - +0.3 123,466 - +0.8 123,859 + MMP_RS00675 0.69 +1.7 124,055 - +0.9 124,094 + -1.0 124,109 + +0.3
Or see this region's nucleotide sequence