Experiment: SMN_rich_noKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A3535 and Rru_A3536 are separated by 107 nucleotides Rru_A3536 and Rru_A3537 are separated by 251 nucleotides Rru_A3537 and Rru_A3538 overlap by 4 nucleotides
Rru_A3535: Rru_A3535 - Tryptophanyl-tRNA synthetase, class Ib (NCBI), at 4,066,271 to 4,067,269
_A3535
Rru_A3536: Rru_A3536 - Nitroreductase (NCBI), at 4,067,377 to 4,068,012
_A3536
Rru_A3537: Rru_A3537 - Predicted transcriptional regulators containing the CopG/Arc/MetJ DNA-binding domain (NCBI), at 4,068,264 to 4,068,560
_A3537
Rru_A3538: Rru_A3538 - Virulence factor MVIN-like (NCBI), at 4,068,557 to 4,070,098
_A3538
Position (kb)
4068
4069 Strain fitness (log2 ratio)
-1
0
1 at 4067.657 kb on - strand, within Rru_A3536 at 4067.777 kb on - strand, within Rru_A3536 at 4067.799 kb on + strand, within Rru_A3536 at 4067.915 kb on - strand, within Rru_A3536 at 4068.051 kb on + strand at 4068.051 kb on + strand at 4068.052 kb on - strand at 4068.064 kb on - strand at 4068.064 kb on - strand at 4068.085 kb on + strand at 4068.085 kb on + strand at 4068.085 kb on + strand at 4068.085 kb on + strand at 4068.086 kb on - strand at 4068.086 kb on - strand at 4068.086 kb on - strand at 4068.086 kb on - strand at 4068.086 kb on - strand at 4068.086 kb on - strand at 4068.086 kb on - strand at 4068.086 kb on - strand at 4068.086 kb on - strand at 4068.200 kb on + strand at 4068.211 kb on + strand at 4068.219 kb on + strand at 4068.220 kb on - strand at 4068.503 kb on - strand, within Rru_A3537 at 4068.503 kb on - strand, within Rru_A3537 at 4068.558 kb on + strand at 4068.559 kb on - strand at 4068.559 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMN_rich_noKan remove 4,067,657 - Rru_A3536 0.44 -0.0 4,067,777 - Rru_A3536 0.63 -0.0 4,067,799 + Rru_A3536 0.66 +0.3 4,067,915 - Rru_A3536 0.85 +0.4 4,068,051 + -0.1 4,068,051 + -0.0 4,068,052 - +0.3 4,068,064 - +0.6 4,068,064 - -0.4 4,068,085 + -0.1 4,068,085 + +0.2 4,068,085 + +1.1 4,068,085 + +0.3 4,068,086 - +0.2 4,068,086 - -0.0 4,068,086 - +0.2 4,068,086 - -1.5 4,068,086 - -0.0 4,068,086 - +0.6 4,068,086 - -0.4 4,068,086 - +0.2 4,068,086 - +0.2 4,068,200 + +0.8 4,068,211 + -0.2 4,068,219 + -0.5 4,068,220 - +1.3 4,068,503 - Rru_A3537 0.80 +0.4 4,068,503 - Rru_A3537 0.80 +0.4 4,068,558 + +0.2 4,068,559 - -0.2 4,068,559 - +0.7
Or see this region's nucleotide sequence