Experiment: SMN_rich_noKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1085 and Rru_A1086 are separated by 273 nucleotides Rru_A1086 and Rru_A1087 overlap by 4 nucleotides Rru_A1087 and Rru_A1088 overlap by 4 nucleotides
Rru_A1085: Rru_A1085 - Metallophosphoesterase (NCBI), at 1,275,623 to 1,276,441
_A1085
Rru_A1086: Rru_A1086 - hypothetical protein (RefSeq), at 1,276,715 to 1,277,464
_A1086
Rru_A1087: Rru_A1087 - Crossover junction endodeoxyribonuclease RuvC (NCBI), at 1,277,461 to 1,277,970
_A1087
Rru_A1088: Rru_A1088 - DNA recombination protein RuvA (NCBI), at 1,277,967 to 1,278,605
_A1088
Position (kb)
1276
1277
1278 Strain fitness (log2 ratio)
-1
0
1 at 1276.206 kb on - strand, within Rru_A1085 at 1276.206 kb on - strand, within Rru_A1085 at 1276.206 kb on - strand, within Rru_A1085 at 1276.207 kb on + strand, within Rru_A1085 at 1276.451 kb on - strand at 1276.579 kb on + strand at 1276.579 kb on + strand at 1276.579 kb on + strand at 1276.699 kb on + strand at 1276.699 kb on + strand at 1276.701 kb on + strand at 1276.906 kb on + strand, within Rru_A1086 at 1276.906 kb on + strand, within Rru_A1086 at 1276.909 kb on + strand, within Rru_A1086 at 1276.973 kb on + strand, within Rru_A1086 at 1276.982 kb on + strand, within Rru_A1086 at 1277.423 kb on + strand at 1277.423 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMN_rich_noKan remove 1,276,206 - Rru_A1085 0.71 -1.7 1,276,206 - Rru_A1085 0.71 -1.7 1,276,206 - Rru_A1085 0.71 -1.3 1,276,207 + Rru_A1085 0.71 -1.7 1,276,451 - +0.6 1,276,579 + +0.1 1,276,579 + +0.9 1,276,579 + +0.2 1,276,699 + -0.5 1,276,699 + +0.4 1,276,701 + +0.2 1,276,906 + Rru_A1086 0.25 -0.7 1,276,906 + Rru_A1086 0.25 -0.4 1,276,909 + Rru_A1086 0.26 -1.3 1,276,973 + Rru_A1086 0.34 -0.8 1,276,982 + Rru_A1086 0.36 -0.2 1,277,423 + +0.8 1,277,423 + -0.9
Or see this region's nucleotide sequence