Experiment: SMN_rich_noKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0169 and Rru_A0170 are separated by 5 nucleotides Rru_A0170 and Rru_A0171 overlap by 4 nucleotides Rru_A0171 and Rru_A0172 are separated by 4 nucleotides
Rru_A0169: Rru_A0169 - Protein of unknown function DUF48 (NCBI), at 200,286 to 201,227
_A0169
Rru_A0170: Rru_A0170 - CRISPR-associated protein, CT1974 (NCBI), at 201,233 to 201,895
_A0170
Rru_A0171: Rru_A0171 - CRISPR-associated protein, CT1976 (NCBI), at 201,892 to 202,677
_A0171
Rru_A0172: Rru_A0172 - CRISPR-associated protein, CT1975 (NCBI), at 202,682 to 203,761
_A0172
Position (kb)
201
202
203 Strain fitness (log2 ratio)
-2
-1
0
1 at 200.922 kb on + strand, within Rru_A0169 at 200.922 kb on + strand, within Rru_A0169 at 200.922 kb on + strand, within Rru_A0169 at 200.923 kb on - strand, within Rru_A0169 at 200.923 kb on - strand, within Rru_A0169 at 200.923 kb on - strand, within Rru_A0169 at 201.138 kb on + strand at 201.234 kb on + strand at 201.235 kb on - strand at 201.235 kb on - strand at 201.235 kb on - strand at 201.273 kb on + strand at 201.273 kb on + strand at 201.274 kb on - strand at 201.351 kb on + strand, within Rru_A0170 at 201.352 kb on - strand, within Rru_A0170 at 201.352 kb on - strand, within Rru_A0170 at 201.352 kb on - strand, within Rru_A0170 at 201.651 kb on + strand, within Rru_A0170 at 201.651 kb on + strand, within Rru_A0170 at 201.651 kb on + strand, within Rru_A0170 at 201.651 kb on + strand, within Rru_A0170 at 201.717 kb on + strand, within Rru_A0170 at 201.718 kb on - strand, within Rru_A0170 at 201.886 kb on + strand at 201.887 kb on - strand at 201.887 kb on - strand at 201.887 kb on - strand at 202.142 kb on + strand, within Rru_A0171 at 202.142 kb on + strand, within Rru_A0171 at 202.229 kb on + strand, within Rru_A0171 at 202.337 kb on + strand, within Rru_A0171 at 202.442 kb on + strand, within Rru_A0171 at 202.443 kb on - strand, within Rru_A0171 at 202.555 kb on + strand, within Rru_A0171 at 202.683 kb on + strand at 202.683 kb on + strand at 202.684 kb on - strand at 202.794 kb on + strand, within Rru_A0172 at 202.794 kb on + strand, within Rru_A0172 at 202.794 kb on + strand, within Rru_A0172 at 202.795 kb on - strand, within Rru_A0172 at 202.795 kb on - strand, within Rru_A0172 at 203.091 kb on + strand, within Rru_A0172 at 203.091 kb on + strand, within Rru_A0172 at 203.091 kb on + strand, within Rru_A0172 at 203.092 kb on - strand, within Rru_A0172 at 203.092 kb on - strand, within Rru_A0172 at 203.098 kb on - strand, within Rru_A0172 at 203.098 kb on - strand, within Rru_A0172 at 203.190 kb on + strand, within Rru_A0172 at 203.190 kb on + strand, within Rru_A0172 at 203.190 kb on + strand, within Rru_A0172 at 203.190 kb on + strand, within Rru_A0172 at 203.191 kb on - strand, within Rru_A0172 at 203.191 kb on - strand, within Rru_A0172 at 203.191 kb on - strand, within Rru_A0172 at 203.191 kb on - strand, within Rru_A0172 at 203.193 kb on + strand, within Rru_A0172 at 203.193 kb on + strand, within Rru_A0172 at 203.193 kb on + strand, within Rru_A0172 at 203.194 kb on - strand, within Rru_A0172
Per-strain Table
Position Strand Gene LocusTag Fraction SMN_rich_noKan remove 200,922 + Rru_A0169 0.68 +0.1 200,922 + Rru_A0169 0.68 -0.5 200,922 + Rru_A0169 0.68 -1.0 200,923 - Rru_A0169 0.68 -0.1 200,923 - Rru_A0169 0.68 +0.1 200,923 - Rru_A0169 0.68 +0.9 201,138 + -0.2 201,234 + +0.3 201,235 - -0.1 201,235 - +0.0 201,235 - +0.1 201,273 + -0.6 201,273 + +0.0 201,274 - -0.4 201,351 + Rru_A0170 0.18 -0.3 201,352 - Rru_A0170 0.18 -0.1 201,352 - Rru_A0170 0.18 +0.0 201,352 - Rru_A0170 0.18 +0.5 201,651 + Rru_A0170 0.63 -0.8 201,651 + Rru_A0170 0.63 +0.6 201,651 + Rru_A0170 0.63 -0.1 201,651 + Rru_A0170 0.63 -0.2 201,717 + Rru_A0170 0.73 +0.4 201,718 - Rru_A0170 0.73 -0.2 201,886 + -0.2 201,887 - +0.1 201,887 - -0.1 201,887 - +0.2 202,142 + Rru_A0171 0.32 -0.4 202,142 + Rru_A0171 0.32 -0.2 202,229 + Rru_A0171 0.43 +0.2 202,337 + Rru_A0171 0.57 +0.5 202,442 + Rru_A0171 0.70 +0.6 202,443 - Rru_A0171 0.70 -0.1 202,555 + Rru_A0171 0.84 +1.1 202,683 + -0.1 202,683 + +0.3 202,684 - +0.1 202,794 + Rru_A0172 0.10 -2.4 202,794 + Rru_A0172 0.10 +0.4 202,794 + Rru_A0172 0.10 -0.2 202,795 - Rru_A0172 0.10 -0.0 202,795 - Rru_A0172 0.10 -1.0 203,091 + Rru_A0172 0.38 +0.0 203,091 + Rru_A0172 0.38 +0.4 203,091 + Rru_A0172 0.38 +0.5 203,092 - Rru_A0172 0.38 +0.2 203,092 - Rru_A0172 0.38 -0.3 203,098 - Rru_A0172 0.39 +0.5 203,098 - Rru_A0172 0.39 +0.1 203,190 + Rru_A0172 0.47 +0.0 203,190 + Rru_A0172 0.47 -0.7 203,190 + Rru_A0172 0.47 +0.5 203,190 + Rru_A0172 0.47 +0.3 203,191 - Rru_A0172 0.47 -0.2 203,191 - Rru_A0172 0.47 +0.2 203,191 - Rru_A0172 0.47 -0.1 203,191 - Rru_A0172 0.47 -0.5 203,193 + Rru_A0172 0.47 -0.4 203,193 + Rru_A0172 0.47 +0.6 203,193 + Rru_A0172 0.47 +0.0 203,194 - Rru_A0172 0.47 -0.2
Or see this region's nucleotide sequence