Experiment: Succinate (C)_noKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2166 and Rru_A2167 are separated by 130 nucleotides Rru_A2167 and Rru_A2168 overlap by 4 nucleotides Rru_A2168 and Rru_A2169 are separated by 99 nucleotides
Rru_A2166: Rru_A2166 - hypothetical protein (NCBI), at 2,514,791 to 2,516,128
_A2166
Rru_A2167: Rru_A2167 - Phosphoribosylformylglycinamidine cyclo-ligase (NCBI), at 2,516,259 to 2,517,425
_A2167
Rru_A2168: Rru_A2168 - Phosphoribosylglycinamide formyltransferase (NCBI), at 2,517,422 to 2,518,096
_A2168
Rru_A2169: Rru_A2169 - Nucleoside-diphosphate kinase (NCBI), at 2,518,196 to 2,518,618
_A2169
Position (kb)
2516
2517
2518 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2515.312 kb on - strand, within Rru_A2166 at 2515.312 kb on - strand, within Rru_A2166 at 2515.312 kb on - strand, within Rru_A2166 at 2515.341 kb on + strand, within Rru_A2166 at 2515.342 kb on - strand, within Rru_A2166 at 2515.342 kb on - strand, within Rru_A2166 at 2515.342 kb on - strand, within Rru_A2166 at 2515.342 kb on - strand, within Rru_A2166 at 2515.342 kb on - strand, within Rru_A2166 at 2515.690 kb on + strand, within Rru_A2166 at 2515.691 kb on - strand, within Rru_A2166 at 2515.691 kb on - strand, within Rru_A2166 at 2515.821 kb on + strand, within Rru_A2166 at 2516.153 kb on - strand at 2516.155 kb on - strand at 2516.155 kb on - strand at 2516.155 kb on - strand at 2516.379 kb on + strand, within Rru_A2167 at 2516.537 kb on + strand, within Rru_A2167 at 2516.679 kb on + strand, within Rru_A2167 at 2516.802 kb on + strand, within Rru_A2167 at 2516.802 kb on + strand, within Rru_A2167 at 2516.817 kb on + strand, within Rru_A2167 at 2517.048 kb on + strand, within Rru_A2167 at 2517.104 kb on + strand, within Rru_A2167 at 2518.197 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinate (C)_noKan remove 2,515,312 - Rru_A2166 0.39 -1.0 2,515,312 - Rru_A2166 0.39 +0.2 2,515,312 - Rru_A2166 0.39 +0.2 2,515,341 + Rru_A2166 0.41 -1.7 2,515,342 - Rru_A2166 0.41 -0.7 2,515,342 - Rru_A2166 0.41 -0.4 2,515,342 - Rru_A2166 0.41 -0.6 2,515,342 - Rru_A2166 0.41 -0.3 2,515,342 - Rru_A2166 0.41 -0.4 2,515,690 + Rru_A2166 0.67 +0.3 2,515,691 - Rru_A2166 0.67 +0.3 2,515,691 - Rru_A2166 0.67 +0.3 2,515,821 + Rru_A2166 0.77 -0.0 2,516,153 - -0.6 2,516,155 - -1.1 2,516,155 - -0.7 2,516,155 - +0.4 2,516,379 + Rru_A2167 0.10 -0.4 2,516,537 + Rru_A2167 0.24 -2.4 2,516,679 + Rru_A2167 0.36 -1.5 2,516,802 + Rru_A2167 0.47 -4.0 2,516,802 + Rru_A2167 0.47 -2.3 2,516,817 + Rru_A2167 0.48 +0.6 2,517,048 + Rru_A2167 0.68 -1.4 2,517,104 + Rru_A2167 0.72 +1.6 2,518,197 + +1.6
Or see this region's nucleotide sequence