Experiment: Succinate (C)_noKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1575 and Rru_A1576 are separated by 99 nucleotides Rru_A1576 and Rru_A1577 are separated by 101 nucleotides Rru_A1577 and Rru_A1578 overlap by 4 nucleotides
Rru_A1575: Rru_A1575 - hypothetical protein (NCBI), at 1,856,117 to 1,856,668
_A1575
Rru_A1576: Rru_A1576 - Prolyl-tRNA synthetase (NCBI), at 1,856,768 to 1,858,075
_A1576
Rru_A1577: Rru_A1577 - ThiJ/PfpI (NCBI), at 1,858,177 to 1,858,806
_A1577
Rru_A1578: Rru_A1578 - Lipoprotein releasing system, transmembrane protein, LolC/E family (NCBI), at 1,858,803 to 1,860,056
_A1578
Position (kb)
1856
1857
1858
1859 Strain fitness (log2 ratio)
-2
-1
0
1 at 1856.007 kb on + strand at 1856.081 kb on - strand at 1856.123 kb on - strand at 1856.148 kb on + strand at 1856.314 kb on + strand, within Rru_A1575 at 1856.315 kb on - strand, within Rru_A1575 at 1856.315 kb on - strand, within Rru_A1575 at 1856.372 kb on + strand, within Rru_A1575 at 1856.372 kb on + strand, within Rru_A1575 at 1856.372 kb on + strand, within Rru_A1575 at 1856.373 kb on - strand, within Rru_A1575 at 1856.373 kb on - strand, within Rru_A1575 at 1856.373 kb on - strand, within Rru_A1575 at 1856.429 kb on + strand, within Rru_A1575 at 1856.430 kb on - strand, within Rru_A1575 at 1856.540 kb on + strand, within Rru_A1575 at 1856.540 kb on + strand, within Rru_A1575 at 1856.540 kb on + strand, within Rru_A1575 at 1856.540 kb on + strand, within Rru_A1575 at 1856.541 kb on - strand, within Rru_A1575 at 1856.541 kb on - strand, within Rru_A1575 at 1856.543 kb on - strand, within Rru_A1575 at 1856.558 kb on + strand, within Rru_A1575 at 1856.558 kb on + strand, within Rru_A1575 at 1856.558 kb on + strand, within Rru_A1575 at 1858.196 kb on + strand at 1858.198 kb on + strand at 1858.198 kb on + strand at 1858.198 kb on + strand at 1858.198 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinate (C)_noKan remove 1,856,007 + -2.2 1,856,081 - +0.4 1,856,123 - +0.3 1,856,148 + -0.2 1,856,314 + Rru_A1575 0.36 -0.3 1,856,315 - Rru_A1575 0.36 -0.4 1,856,315 - Rru_A1575 0.36 +0.8 1,856,372 + Rru_A1575 0.46 -0.6 1,856,372 + Rru_A1575 0.46 +1.2 1,856,372 + Rru_A1575 0.46 -1.3 1,856,373 - Rru_A1575 0.46 -0.4 1,856,373 - Rru_A1575 0.46 -0.2 1,856,373 - Rru_A1575 0.46 +0.2 1,856,429 + Rru_A1575 0.57 -0.4 1,856,430 - Rru_A1575 0.57 -1.6 1,856,540 + Rru_A1575 0.77 -1.9 1,856,540 + Rru_A1575 0.77 +0.5 1,856,540 + Rru_A1575 0.77 +0.2 1,856,540 + Rru_A1575 0.77 +0.3 1,856,541 - Rru_A1575 0.77 -0.9 1,856,541 - Rru_A1575 0.77 +0.3 1,856,543 - Rru_A1575 0.77 -1.5 1,856,558 + Rru_A1575 0.80 -0.2 1,856,558 + Rru_A1575 0.80 -0.9 1,856,558 + Rru_A1575 0.80 -1.0 1,858,196 + +0.5 1,858,198 + -0.8 1,858,198 + +0.7 1,858,198 + -0.2 1,858,198 + +0.1
Or see this region's nucleotide sequence