Experiment: L-Threonine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c00740 and PGA1_c00750 are separated by 188 nucleotides PGA1_c00750 and PGA1_c00760 are separated by 36 nucleotides PGA1_c00760 and PGA1_c00770 are separated by 158 nucleotides PGA1_c00770 and PGA1_c00780 are separated by 123 nucleotides
PGA1_c00740: PGA1_c00740 - ATP synthase B chain, at 72,164 to 72,724
_c00740
PGA1_c00750: PGA1_c00750 - UDP-N-acetylglucosamine acyltransferase-like protein, at 72,913 to 73,662
_c00750
PGA1_c00760: PGA1_c00760 - putative pyruvate dehydrogenase complex repressor, at 73,699 to 74,469
_c00760
PGA1_c00770: PGA1_c00770 - hypothetical protein, at 74,628 to 74,966
_c00770
PGA1_c00780: PGA1_c00780 - putative chromosome segregation protein SMC, at 75,090 to 78,545
_c00780
Position (kb) 73
74
75 Strain fitness (log2 ratio) -2
-1
0
1 at 72.706 kb on - strand at 72.718 kb on + strand at 72.742 kb on - strand at 72.810 kb on - strand at 72.812 kb on - strand at 72.857 kb on - strand at 72.869 kb on + strand at 72.877 kb on - strand at 72.879 kb on + strand at 72.887 kb on - strand at 72.949 kb on + strand at 72.953 kb on + strand at 72.956 kb on - strand at 73.032 kb on - strand, within PGA1_c00750 at 73.073 kb on + strand, within PGA1_c00750 at 73.073 kb on + strand, within PGA1_c00750 at 73.073 kb on + strand, within PGA1_c00750 at 73.073 kb on + strand, within PGA1_c00750 at 73.073 kb on + strand, within PGA1_c00750 at 73.073 kb on + strand, within PGA1_c00750 at 73.081 kb on - strand, within PGA1_c00750 at 73.081 kb on - strand, within PGA1_c00750 at 73.083 kb on + strand, within PGA1_c00750 at 73.083 kb on + strand, within PGA1_c00750 at 73.083 kb on + strand, within PGA1_c00750 at 73.177 kb on - strand, within PGA1_c00750 at 73.230 kb on + strand, within PGA1_c00750 at 73.249 kb on + strand, within PGA1_c00750 at 73.255 kb on - strand, within PGA1_c00750 at 73.256 kb on + strand, within PGA1_c00750 at 73.257 kb on - strand, within PGA1_c00750 at 73.282 kb on - strand, within PGA1_c00750 at 73.282 kb on - strand, within PGA1_c00750 at 73.285 kb on - strand, within PGA1_c00750 at 73.288 kb on + strand, within PGA1_c00750 at 73.296 kb on - strand, within PGA1_c00750 at 73.331 kb on + strand, within PGA1_c00750 at 73.339 kb on - strand, within PGA1_c00750 at 73.375 kb on - strand, within PGA1_c00750 at 73.377 kb on - strand, within PGA1_c00750 at 73.377 kb on - strand, within PGA1_c00750 at 73.434 kb on + strand, within PGA1_c00750 at 73.458 kb on + strand, within PGA1_c00750 at 73.458 kb on + strand, within PGA1_c00750 at 73.458 kb on + strand, within PGA1_c00750 at 73.466 kb on - strand, within PGA1_c00750 at 73.533 kb on + strand, within PGA1_c00750 at 73.538 kb on - strand, within PGA1_c00750 at 73.579 kb on + strand, within PGA1_c00750 at 73.579 kb on + strand, within PGA1_c00750 at 73.583 kb on + strand, within PGA1_c00750 at 73.658 kb on - strand at 73.674 kb on - strand at 73.703 kb on - strand at 73.710 kb on - strand at 73.712 kb on + strand at 73.740 kb on + strand at 73.782 kb on + strand, within PGA1_c00760 at 73.782 kb on + strand, within PGA1_c00760 at 73.790 kb on - strand, within PGA1_c00760 at 73.790 kb on - strand, within PGA1_c00760 at 73.800 kb on - strand, within PGA1_c00760 at 73.800 kb on - strand, within PGA1_c00760 at 73.801 kb on + strand, within PGA1_c00760 at 73.864 kb on + strand, within PGA1_c00760 at 73.864 kb on + strand, within PGA1_c00760 at 73.864 kb on + strand, within PGA1_c00760 at 73.939 kb on - strand, within PGA1_c00760 at 74.037 kb on + strand, within PGA1_c00760 at 74.045 kb on - strand, within PGA1_c00760 at 74.045 kb on - strand, within PGA1_c00760 at 74.045 kb on - strand, within PGA1_c00760 at 74.064 kb on + strand, within PGA1_c00760 at 74.064 kb on + strand, within PGA1_c00760 at 74.231 kb on + strand, within PGA1_c00760 at 74.246 kb on - strand, within PGA1_c00760 at 74.346 kb on + strand, within PGA1_c00760 at 74.363 kb on + strand, within PGA1_c00760 at 74.364 kb on - strand, within PGA1_c00760 at 74.381 kb on + strand, within PGA1_c00760 at 74.381 kb on + strand, within PGA1_c00760 at 74.381 kb on + strand, within PGA1_c00760 at 74.389 kb on - strand, within PGA1_c00760 at 74.400 kb on + strand at 74.424 kb on + strand at 74.424 kb on + strand at 74.432 kb on - strand at 74.432 kb on - strand at 74.437 kb on + strand at 74.448 kb on + strand at 74.473 kb on - strand at 74.473 kb on - strand at 74.504 kb on - strand at 74.525 kb on - strand at 74.525 kb on - strand at 74.535 kb on + strand at 74.536 kb on - strand at 74.536 kb on - strand at 74.536 kb on - strand at 74.573 kb on + strand at 74.586 kb on - strand at 74.619 kb on + strand at 74.619 kb on + strand at 74.619 kb on + strand at 74.622 kb on + strand at 74.622 kb on - strand at 74.627 kb on - strand at 74.628 kb on - strand at 74.628 kb on - strand at 74.628 kb on - strand at 74.629 kb on + strand at 74.647 kb on - strand at 74.660 kb on - strand at 74.696 kb on - strand, within PGA1_c00770 at 74.710 kb on + strand, within PGA1_c00770 at 74.754 kb on + strand, within PGA1_c00770 at 74.754 kb on - strand, within PGA1_c00770 at 74.762 kb on - strand, within PGA1_c00770 at 74.764 kb on - strand, within PGA1_c00770 at 74.775 kb on - strand, within PGA1_c00770 at 74.801 kb on + strand, within PGA1_c00770 at 74.834 kb on + strand, within PGA1_c00770 at 74.892 kb on - strand, within PGA1_c00770 at 74.892 kb on - strand, within PGA1_c00770 at 74.901 kb on + strand, within PGA1_c00770 at 74.901 kb on + strand, within PGA1_c00770 at 74.909 kb on - strand, within PGA1_c00770 at 74.909 kb on - strand, within PGA1_c00770 at 74.948 kb on + strand at 75.001 kb on - strand at 75.012 kb on + strand at 75.200 kb on + strand at 75.224 kb on - strand at 75.307 kb on - strand at 75.333 kb on - strand at 75.404 kb on - strand at 75.445 kb on + strand, within PGA1_c00780 at 75.445 kb on + strand, within PGA1_c00780
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (C) remove 72,706 - +0.0 72,718 + +0.3 72,742 - -0.2 72,810 - +0.1 72,812 - +0.7 72,857 - +0.2 72,869 + -1.9 72,877 - -0.2 72,879 + +0.3 72,887 - -0.2 72,949 + +0.1 72,953 + +0.7 72,956 - +0.1 73,032 - PGA1_c00750 0.16 +0.9 73,073 + PGA1_c00750 0.21 -0.7 73,073 + PGA1_c00750 0.21 -1.0 73,073 + PGA1_c00750 0.21 -0.5 73,073 + PGA1_c00750 0.21 +0.5 73,073 + PGA1_c00750 0.21 +0.3 73,073 + PGA1_c00750 0.21 -0.2 73,081 - PGA1_c00750 0.22 -0.6 73,081 - PGA1_c00750 0.22 -0.4 73,083 + PGA1_c00750 0.23 -0.1 73,083 + PGA1_c00750 0.23 +0.9 73,083 + PGA1_c00750 0.23 +0.4 73,177 - PGA1_c00750 0.35 +0.4 73,230 + PGA1_c00750 0.42 +0.5 73,249 + PGA1_c00750 0.45 +1.7 73,255 - PGA1_c00750 0.46 +0.5 73,256 + PGA1_c00750 0.46 +0.1 73,257 - PGA1_c00750 0.46 -1.2 73,282 - PGA1_c00750 0.49 +0.1 73,282 - PGA1_c00750 0.49 +0.6 73,285 - PGA1_c00750 0.50 -0.6 73,288 + PGA1_c00750 0.50 -0.4 73,296 - PGA1_c00750 0.51 +0.3 73,331 + PGA1_c00750 0.56 -0.2 73,339 - PGA1_c00750 0.57 +0.9 73,375 - PGA1_c00750 0.62 +0.3 73,377 - PGA1_c00750 0.62 -0.8 73,377 - PGA1_c00750 0.62 -0.2 73,434 + PGA1_c00750 0.69 -1.1 73,458 + PGA1_c00750 0.73 +0.8 73,458 + PGA1_c00750 0.73 +0.4 73,458 + PGA1_c00750 0.73 +1.0 73,466 - PGA1_c00750 0.74 -1.0 73,533 + PGA1_c00750 0.83 -0.5 73,538 - PGA1_c00750 0.83 -0.2 73,579 + PGA1_c00750 0.89 -0.9 73,579 + PGA1_c00750 0.89 -0.7 73,583 + PGA1_c00750 0.89 -0.2 73,658 - +0.7 73,674 - +0.0 73,703 - +0.1 73,710 - +0.3 73,712 + -0.5 73,740 + +0.4 73,782 + PGA1_c00760 0.11 -1.5 73,782 + PGA1_c00760 0.11 -0.9 73,790 - PGA1_c00760 0.12 -0.5 73,790 - PGA1_c00760 0.12 +0.4 73,800 - PGA1_c00760 0.13 +0.1 73,800 - PGA1_c00760 0.13 -2.2 73,801 + PGA1_c00760 0.13 -0.2 73,864 + PGA1_c00760 0.21 -2.2 73,864 + PGA1_c00760 0.21 -0.4 73,864 + PGA1_c00760 0.21 -1.3 73,939 - PGA1_c00760 0.31 -1.0 74,037 + PGA1_c00760 0.44 -0.7 74,045 - PGA1_c00760 0.45 -0.8 74,045 - PGA1_c00760 0.45 -1.5 74,045 - PGA1_c00760 0.45 +0.2 74,064 + PGA1_c00760 0.47 -1.7 74,064 + PGA1_c00760 0.47 -1.7 74,231 + PGA1_c00760 0.69 -2.1 74,246 - PGA1_c00760 0.71 -2.4 74,346 + PGA1_c00760 0.84 +0.2 74,363 + PGA1_c00760 0.86 -2.4 74,364 - PGA1_c00760 0.86 +0.1 74,381 + PGA1_c00760 0.88 -0.2 74,381 + PGA1_c00760 0.88 -0.5 74,381 + PGA1_c00760 0.88 -0.9 74,389 - PGA1_c00760 0.89 -0.5 74,400 + -0.5 74,424 + -0.6 74,424 + +0.0 74,432 - -0.1 74,432 - +0.2 74,437 + -0.4 74,448 + -0.1 74,473 - +0.1 74,473 - -0.9 74,504 - -0.0 74,525 - -0.7 74,525 - -0.4 74,535 + -0.4 74,536 - -1.7 74,536 - -0.6 74,536 - -0.5 74,573 + +0.4 74,586 - +0.7 74,619 + +0.7 74,619 + -0.5 74,619 + -0.4 74,622 + -0.3 74,622 - -0.0 74,627 - -0.9 74,628 - -0.0 74,628 - +1.4 74,628 - -0.2 74,629 + -1.4 74,647 - +0.1 74,660 - +0.0 74,696 - PGA1_c00770 0.20 +0.7 74,710 + PGA1_c00770 0.24 +0.2 74,754 + PGA1_c00770 0.37 -0.3 74,754 - PGA1_c00770 0.37 +0.5 74,762 - PGA1_c00770 0.40 +0.2 74,764 - PGA1_c00770 0.40 -1.9 74,775 - PGA1_c00770 0.43 +0.6 74,801 + PGA1_c00770 0.51 +0.1 74,834 + PGA1_c00770 0.61 +0.2 74,892 - PGA1_c00770 0.78 +0.6 74,892 - PGA1_c00770 0.78 +0.3 74,901 + PGA1_c00770 0.81 +0.8 74,901 + PGA1_c00770 0.81 +1.0 74,909 - PGA1_c00770 0.83 +0.4 74,909 - PGA1_c00770 0.83 -0.3 74,948 + +0.5 75,001 - +0.0 75,012 + -0.5 75,200 + +0.4 75,224 - +0.5 75,307 - -0.2 75,333 - +1.5 75,404 - +0.4 75,445 + PGA1_c00780 0.10 +0.3 75,445 + PGA1_c00780 0.10 +0.9
Or see this region's nucleotide sequence