Experiment: L-Threonine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c02120 and PGA1_c02130 are separated by 5 nucleotides PGA1_c02130 and PGA1_c02140 are separated by 99 nucleotides PGA1_c02140 and PGA1_c02150 are separated by 26 nucleotides PGA1_c02150 and PGA1_c02170 are separated by 398 nucleotides
PGA1_c02120: PGA1_c02120 - hypothetical protein, at 195,571 to 196,143
_c02120
PGA1_c02130: PGA1_c02130 - conserved hypothetical protein YidD, at 196,149 to 196,421
_c02130
PGA1_c02140: PGA1_c02140 - putative ribonuclease P component, at 196,521 to 196,973
_c02140
PGA1_c02150: PGA1_c02150 - 50S ribosomal protein L34, at 197,000 to 197,134
_c02150
PGA1_c02170: PGA1_c02170 - Uncharacterized conserved protein, at 197,533 to 198,336
_c02170
Position (kb)
196
197 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 195.530 kb on + strand at 195.556 kb on + strand at 195.556 kb on + strand at 195.562 kb on + strand at 195.562 kb on + strand at 195.564 kb on - strand at 195.570 kb on - strand at 195.617 kb on + strand at 195.672 kb on + strand, within PGA1_c02120 at 195.768 kb on + strand, within PGA1_c02120 at 195.768 kb on + strand, within PGA1_c02120 at 195.776 kb on - strand, within PGA1_c02120 at 195.776 kb on - strand, within PGA1_c02120 at 195.776 kb on - strand, within PGA1_c02120 at 195.778 kb on + strand, within PGA1_c02120 at 195.778 kb on + strand, within PGA1_c02120 at 195.786 kb on - strand, within PGA1_c02120 at 195.786 kb on - strand, within PGA1_c02120 at 195.788 kb on + strand, within PGA1_c02120 at 195.796 kb on - strand, within PGA1_c02120 at 195.821 kb on + strand, within PGA1_c02120 at 195.821 kb on + strand, within PGA1_c02120 at 195.821 kb on + strand, within PGA1_c02120 at 195.829 kb on - strand, within PGA1_c02120 at 195.829 kb on - strand, within PGA1_c02120 at 195.829 kb on - strand, within PGA1_c02120 at 195.857 kb on - strand, within PGA1_c02120 at 195.866 kb on + strand, within PGA1_c02120 at 195.891 kb on + strand, within PGA1_c02120 at 195.942 kb on - strand, within PGA1_c02120 at 195.971 kb on - strand, within PGA1_c02120 at 195.979 kb on - strand, within PGA1_c02120 at 196.060 kb on + strand, within PGA1_c02120 at 196.084 kb on + strand, within PGA1_c02120 at 196.100 kb on + strand at 196.108 kb on - strand at 196.118 kb on - strand at 196.127 kb on + strand at 196.147 kb on + strand at 196.198 kb on - strand, within PGA1_c02130 at 196.211 kb on - strand, within PGA1_c02130 at 196.360 kb on + strand, within PGA1_c02130 at 196.373 kb on + strand, within PGA1_c02130 at 196.417 kb on + strand at 196.423 kb on - strand at 196.448 kb on + strand at 196.466 kb on - strand at 196.495 kb on - strand at 196.504 kb on + strand at 196.504 kb on + strand at 196.504 kb on + strand at 196.512 kb on - strand at 196.977 kb on + strand at 197.335 kb on + strand at 197.368 kb on + strand at 197.370 kb on - strand at 197.376 kb on - strand at 197.412 kb on - strand at 197.470 kb on - strand at 197.486 kb on + strand at 197.486 kb on + strand at 197.486 kb on + strand at 197.501 kb on + strand at 197.507 kb on + strand at 197.509 kb on - strand at 197.515 kb on - strand at 197.621 kb on + strand, within PGA1_c02170 at 197.621 kb on + strand, within PGA1_c02170 at 197.629 kb on - strand, within PGA1_c02170 at 197.629 kb on - strand, within PGA1_c02170 at 197.629 kb on - strand, within PGA1_c02170 at 197.681 kb on + strand, within PGA1_c02170 at 197.687 kb on + strand, within PGA1_c02170 at 197.771 kb on + strand, within PGA1_c02170 at 197.785 kb on + strand, within PGA1_c02170 at 197.785 kb on + strand, within PGA1_c02170 at 197.790 kb on + strand, within PGA1_c02170 at 197.790 kb on + strand, within PGA1_c02170 at 197.790 kb on + strand, within PGA1_c02170 at 197.790 kb on + strand, within PGA1_c02170 at 197.790 kb on + strand, within PGA1_c02170 at 197.792 kb on + strand, within PGA1_c02170 at 197.793 kb on - strand, within PGA1_c02170 at 197.867 kb on - strand, within PGA1_c02170 at 197.867 kb on - strand, within PGA1_c02170 at 197.942 kb on + strand, within PGA1_c02170
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (C) remove 195,530 + +0.2 195,556 + -1.9 195,556 + -0.2 195,562 + +0.2 195,562 + +1.1 195,564 - -0.0 195,570 - +0.0 195,617 + -0.5 195,672 + PGA1_c02120 0.18 +0.1 195,768 + PGA1_c02120 0.34 +0.4 195,768 + PGA1_c02120 0.34 +0.7 195,776 - PGA1_c02120 0.36 +0.1 195,776 - PGA1_c02120 0.36 +0.7 195,776 - PGA1_c02120 0.36 +1.0 195,778 + PGA1_c02120 0.36 -1.0 195,778 + PGA1_c02120 0.36 -0.1 195,786 - PGA1_c02120 0.38 -0.5 195,786 - PGA1_c02120 0.38 +0.9 195,788 + PGA1_c02120 0.38 +0.4 195,796 - PGA1_c02120 0.39 +0.8 195,821 + PGA1_c02120 0.44 +0.3 195,821 + PGA1_c02120 0.44 +0.9 195,821 + PGA1_c02120 0.44 +1.1 195,829 - PGA1_c02120 0.45 +0.4 195,829 - PGA1_c02120 0.45 -0.2 195,829 - PGA1_c02120 0.45 -0.1 195,857 - PGA1_c02120 0.50 +1.1 195,866 + PGA1_c02120 0.51 +0.8 195,891 + PGA1_c02120 0.56 -0.3 195,942 - PGA1_c02120 0.65 -0.3 195,971 - PGA1_c02120 0.70 +0.9 195,979 - PGA1_c02120 0.71 +0.3 196,060 + PGA1_c02120 0.85 +0.8 196,084 + PGA1_c02120 0.90 -0.1 196,100 + +1.3 196,108 - +0.2 196,118 - +1.0 196,127 + +0.4 196,147 + +0.1 196,198 - PGA1_c02130 0.18 -0.3 196,211 - PGA1_c02130 0.23 +0.1 196,360 + PGA1_c02130 0.77 +0.2 196,373 + PGA1_c02130 0.82 +0.3 196,417 + -0.8 196,423 - -0.6 196,448 + -0.0 196,466 - +0.6 196,495 - -0.2 196,504 + +1.4 196,504 + -0.3 196,504 + +0.1 196,512 - +0.2 196,977 + +1.8 197,335 + +0.1 197,368 + +0.6 197,370 - +0.2 197,376 - -0.1 197,412 - -0.2 197,470 - +0.5 197,486 + +0.7 197,486 + +0.2 197,486 + +0.0 197,501 + +0.5 197,507 + -0.0 197,509 - +0.8 197,515 - +0.3 197,621 + PGA1_c02170 0.11 -0.2 197,621 + PGA1_c02170 0.11 -0.5 197,629 - PGA1_c02170 0.12 +0.7 197,629 - PGA1_c02170 0.12 +0.6 197,629 - PGA1_c02170 0.12 +0.3 197,681 + PGA1_c02170 0.18 -0.2 197,687 + PGA1_c02170 0.19 -0.4 197,771 + PGA1_c02170 0.30 -0.2 197,785 + PGA1_c02170 0.31 -0.2 197,785 + PGA1_c02170 0.31 -0.3 197,790 + PGA1_c02170 0.32 -0.8 197,790 + PGA1_c02170 0.32 +0.0 197,790 + PGA1_c02170 0.32 +0.3 197,790 + PGA1_c02170 0.32 +0.0 197,790 + PGA1_c02170 0.32 -0.1 197,792 + PGA1_c02170 0.32 +0.1 197,793 - PGA1_c02170 0.32 -0.2 197,867 - PGA1_c02170 0.42 +0.2 197,867 - PGA1_c02170 0.42 -1.0 197,942 + PGA1_c02170 0.51 +0.2
Or see this region's nucleotide sequence