Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS24300

Experiment: LB, pH5

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS24295 and BWI76_RS24300 are separated by 196 nucleotidesBWI76_RS24300 and BWI76_RS24305 are separated by 132 nucleotides BWI76_RS24295: BWI76_RS24295 - AraC family transcriptional regulator, at 5,061,333 to 5,062,232 _RS24295 BWI76_RS24300: BWI76_RS24300 - NADH-dependent alcohol dehydrogenase, at 5,062,429 to 5,063,592 _RS24300 BWI76_RS24305: BWI76_RS24305 - 2,5-didehydrogluconate reductase A, at 5,063,725 to 5,064,552 _RS24305 Position (kb) 5062 5063 5064Strain fitness (log2 ratio) -2 -1 0 1 2at 5061.432 kb on - strand, within BWI76_RS24295at 5061.572 kb on + strand, within BWI76_RS24295at 5061.662 kb on - strandat 5061.719 kb on + strand, within BWI76_RS24295at 5061.953 kb on + strand, within BWI76_RS24295at 5062.013 kb on + strand, within BWI76_RS24295at 5062.126 kb on - strand, within BWI76_RS24295at 5062.149 kb on - strandat 5062.252 kb on - strandat 5062.717 kb on + strand, within BWI76_RS24300at 5062.763 kb on - strand, within BWI76_RS24300at 5062.973 kb on - strand, within BWI76_RS24300at 5063.100 kb on + strand, within BWI76_RS24300at 5063.108 kb on - strand, within BWI76_RS24300at 5063.214 kb on + strand, within BWI76_RS24300at 5063.272 kb on + strand, within BWI76_RS24300at 5063.389 kb on - strand, within BWI76_RS24300at 5063.638 kb on - strandat 5063.646 kb on + strandat 5063.666 kb on + strandat 5063.674 kb on - strandat 5063.710 kb on - strandat 5063.763 kb on + strandat 5063.783 kb on - strandat 5063.910 kb on - strand, within BWI76_RS24305at 5063.912 kb on + strand, within BWI76_RS24305at 5063.920 kb on - strand, within BWI76_RS24305at 5063.976 kb on + strand, within BWI76_RS24305at 5063.976 kb on + strand, within BWI76_RS24305at 5064.083 kb on + strand, within BWI76_RS24305at 5064.307 kb on + strand, within BWI76_RS24305at 5064.385 kb on + strand, within BWI76_RS24305at 5064.385 kb on + strand, within BWI76_RS24305at 5064.385 kb on + strand, within BWI76_RS24305at 5064.510 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB, pH5
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5,061,432 - BWI76_RS24295 0.11 -0.2
5,061,572 + BWI76_RS24295 0.27 -1.1
5,061,662 - -0.6
5,061,719 + BWI76_RS24295 0.43 -1.8
5,061,953 + BWI76_RS24295 0.69 -0.2
5,062,013 + BWI76_RS24295 0.76 +0.2
5,062,126 - BWI76_RS24295 0.88 +2.2
5,062,149 - +0.6
5,062,252 - -0.1
5,062,717 + BWI76_RS24300 0.25 +0.7
5,062,763 - BWI76_RS24300 0.29 +0.2
5,062,973 - BWI76_RS24300 0.47 +0.4
5,063,100 + BWI76_RS24300 0.58 +0.0
5,063,108 - BWI76_RS24300 0.58 -0.2
5,063,214 + BWI76_RS24300 0.67 +0.2
5,063,272 + BWI76_RS24300 0.72 +1.0
5,063,389 - BWI76_RS24300 0.82 +0.6
5,063,638 - -1.5
5,063,646 + -0.1
5,063,666 + -0.0
5,063,674 - +1.2
5,063,710 - +0.6
5,063,763 + +0.1
5,063,783 - +1.0
5,063,910 - BWI76_RS24305 0.22 -0.2
5,063,912 + BWI76_RS24305 0.23 +2.5
5,063,920 - BWI76_RS24305 0.24 +1.4
5,063,976 + BWI76_RS24305 0.30 +1.3
5,063,976 + BWI76_RS24305 0.30 +0.9
5,064,083 + BWI76_RS24305 0.43 -0.5
5,064,307 + BWI76_RS24305 0.70 +0.8
5,064,385 + BWI76_RS24305 0.80 -0.3
5,064,385 + BWI76_RS24305 0.80 -0.0
5,064,385 + BWI76_RS24305 0.80 -0.3
5,064,510 + +0.2

Or see this region's nucleotide sequence