Experiment: Succinate (C)_noKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0467 and Rru_A0468 are separated by 127 nucleotides Rru_A0468 and Rru_A0469 are separated by 126 nucleotides Rru_A0469 and Rru_A0470 are separated by 781 nucleotides
Rru_A0467: Rru_A0467 - Acetolactate synthase, large subunit, biosynthetic type (NCBI), at 547,496 to 549,256
_A0467
Rru_A0468: Rru_A0468 - Acetolactate synthase, small subunit (NCBI), at 549,384 to 549,911
_A0468
Rru_A0469: Rru_A0469 - Ketol-acid reductoisomerase (NCBI), at 550,038 to 551,057
_A0469
Rru_A0470: Rru_A0470 - Cell shape determining protein MreB/Mrl (NCBI), at 551,839 to 552,867
_A0470
Position (kb) 550
551
552 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 549.254 kb on + strand at 549.428 kb on + strand at 549.429 kb on - strand at 549.501 kb on + strand, within Rru_A0468 at 549.503 kb on + strand, within Rru_A0468 at 549.503 kb on + strand, within Rru_A0468 at 549.503 kb on + strand, within Rru_A0468 at 549.504 kb on - strand, within Rru_A0468 at 549.909 kb on + strand at 549.910 kb on - strand at 549.910 kb on - strand at 549.918 kb on - strand at 549.960 kb on - strand at 550.048 kb on - strand at 550.048 kb on - strand at 550.107 kb on + strand at 550.136 kb on + strand at 550.137 kb on - strand at 550.305 kb on + strand, within Rru_A0469 at 550.305 kb on + strand, within Rru_A0469 at 550.305 kb on + strand, within Rru_A0469 at 550.305 kb on + strand, within Rru_A0469 at 550.306 kb on - strand, within Rru_A0469 at 550.306 kb on - strand, within Rru_A0469 at 550.306 kb on - strand, within Rru_A0469 at 550.306 kb on - strand, within Rru_A0469 at 550.306 kb on - strand, within Rru_A0469 at 550.308 kb on - strand, within Rru_A0469 at 550.308 kb on - strand, within Rru_A0469 at 550.328 kb on + strand, within Rru_A0469 at 550.329 kb on - strand, within Rru_A0469 at 550.329 kb on - strand, within Rru_A0469 at 550.458 kb on + strand, within Rru_A0469 at 550.458 kb on + strand, within Rru_A0469 at 550.458 kb on + strand, within Rru_A0469 at 550.459 kb on - strand, within Rru_A0469 at 550.459 kb on - strand, within Rru_A0469 at 550.459 kb on - strand, within Rru_A0469 at 550.539 kb on + strand, within Rru_A0469 at 550.539 kb on + strand, within Rru_A0469 at 550.540 kb on - strand, within Rru_A0469 at 550.693 kb on - strand, within Rru_A0469 at 550.711 kb on - strand, within Rru_A0469 at 550.755 kb on + strand, within Rru_A0469 at 550.755 kb on + strand, within Rru_A0469 at 550.756 kb on - strand, within Rru_A0469 at 550.775 kb on + strand, within Rru_A0469 at 550.776 kb on - strand, within Rru_A0469 at 550.776 kb on - strand, within Rru_A0469 at 550.776 kb on - strand, within Rru_A0469 at 550.815 kb on + strand, within Rru_A0469 at 550.815 kb on + strand, within Rru_A0469 at 550.816 kb on - strand, within Rru_A0469 at 550.816 kb on - strand, within Rru_A0469 at 550.816 kb on - strand, within Rru_A0469 at 550.816 kb on - strand, within Rru_A0469 at 550.816 kb on - strand, within Rru_A0469 at 551.108 kb on + strand at 551.109 kb on - strand at 551.109 kb on - strand at 551.488 kb on + strand at 551.488 kb on + strand at 551.737 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinate (C)_noKan remove 549,254 + +0.0 549,428 + -4.6 549,429 - -4.6 549,501 + Rru_A0468 0.22 -1.8 549,503 + Rru_A0468 0.23 -2.3 549,503 + Rru_A0468 0.23 -5.8 549,503 + Rru_A0468 0.23 -2.6 549,504 - Rru_A0468 0.23 -4.8 549,909 + -0.3 549,910 - -1.8 549,910 - -0.7 549,918 - -1.4 549,960 - -1.0 550,048 - -2.7 550,048 - -4.4 550,107 + -5.0 550,136 + -2.7 550,137 - -3.6 550,305 + Rru_A0469 0.26 -2.6 550,305 + Rru_A0469 0.26 -4.9 550,305 + Rru_A0469 0.26 -4.8 550,305 + Rru_A0469 0.26 -4.0 550,306 - Rru_A0469 0.26 -4.4 550,306 - Rru_A0469 0.26 -5.2 550,306 - Rru_A0469 0.26 -2.6 550,306 - Rru_A0469 0.26 -3.6 550,306 - Rru_A0469 0.26 -2.0 550,308 - Rru_A0469 0.26 -2.8 550,308 - Rru_A0469 0.26 -2.2 550,328 + Rru_A0469 0.28 -3.4 550,329 - Rru_A0469 0.29 -2.3 550,329 - Rru_A0469 0.29 -1.6 550,458 + Rru_A0469 0.41 -4.2 550,458 + Rru_A0469 0.41 -0.9 550,458 + Rru_A0469 0.41 -4.4 550,459 - Rru_A0469 0.41 -3.8 550,459 - Rru_A0469 0.41 -3.4 550,459 - Rru_A0469 0.41 -3.2 550,539 + Rru_A0469 0.49 -5.0 550,539 + Rru_A0469 0.49 -4.2 550,540 - Rru_A0469 0.49 -3.0 550,693 - Rru_A0469 0.64 -4.8 550,711 - Rru_A0469 0.66 -3.9 550,755 + Rru_A0469 0.70 -2.5 550,755 + Rru_A0469 0.70 -3.3 550,756 - Rru_A0469 0.70 -2.2 550,775 + Rru_A0469 0.72 -3.1 550,776 - Rru_A0469 0.72 -5.0 550,776 - Rru_A0469 0.72 -4.7 550,776 - Rru_A0469 0.72 -4.6 550,815 + Rru_A0469 0.76 -2.3 550,815 + Rru_A0469 0.76 -1.6 550,816 - Rru_A0469 0.76 -3.6 550,816 - Rru_A0469 0.76 -2.6 550,816 - Rru_A0469 0.76 -5.3 550,816 - Rru_A0469 0.76 -2.9 550,816 - Rru_A0469 0.76 -3.5 551,108 + -1.4 551,109 - -0.7 551,109 - -1.0 551,488 + +0.5 551,488 + -0.3 551,737 + +0.4
Or see this region's nucleotide sequence