Experiment: Succinate (C)_noKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A3591 and Rru_A3592 are separated by 107 nucleotides Rru_A3592 and Rru_A3593 are separated by 24 nucleotides Rru_A3593 and Rru_A3594 are separated by 73 nucleotides Rru_A3594 and Rru_A3595 are separated by 16 nucleotides Rru_A3595 and Rru_A3596 are separated by 61 nucleotides
Rru_A3591: Rru_A3591 - hypothetical protein (NCBI), at 4,134,776 to 4,135,495
_A3591
Rru_A3592: Rru_A3592 - Histidine triad (HIT) protein (NCBI), at 4,135,603 to 4,135,965
_A3592
Rru_A3593: Rru_A3593 - Phosphoribosyl-ATP pyrophosphohydrolase (NCBI), at 4,135,990 to 4,136,343
_A3593
Rru_A3594: Rru_A3594 - Histidine biosynthesis protein HisF (NCBI), at 4,136,417 to 4,137,175
_A3594
Rru_A3595: Rru_A3595 - Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (NCBI), at 4,137,192 to 4,137,917
_A3595
Rru_A3596: Rru_A3596 - Imidazole glycerol phosphate synthase, glutamine amidotransferase subunit (NCBI), at 4,137,979 to 4,138,623
_A3596
Position (kb)
4136
4137
4138 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4135.434 kb on + strand at 4135.434 kb on + strand at 4135.944 kb on + strand at 4135.944 kb on + strand at 4135.944 kb on + strand at 4135.944 kb on + strand at 4135.944 kb on + strand at 4135.945 kb on - strand at 4135.945 kb on - strand at 4135.945 kb on - strand at 4135.945 kb on - strand at 4135.958 kb on + strand at 4135.959 kb on - strand at 4136.105 kb on + strand, within Rru_A3593 at 4136.238 kb on - strand, within Rru_A3593 at 4136.419 kb on - strand at 4136.449 kb on + strand at 4136.473 kb on + strand at 4136.474 kb on - strand at 4136.475 kb on + strand at 4136.557 kb on + strand, within Rru_A3594 at 4136.557 kb on + strand, within Rru_A3594 at 4136.627 kb on - strand, within Rru_A3594 at 4137.060 kb on + strand, within Rru_A3594 at 4137.929 kb on - strand at 4138.148 kb on + strand, within Rru_A3596 at 4138.149 kb on - strand, within Rru_A3596 at 4138.149 kb on - strand, within Rru_A3596 at 4138.152 kb on + strand, within Rru_A3596 at 4138.153 kb on - strand, within Rru_A3596 at 4138.153 kb on - strand, within Rru_A3596 at 4138.153 kb on - strand, within Rru_A3596 at 4138.153 kb on - strand, within Rru_A3596 at 4138.153 kb on - strand, within Rru_A3596 at 4138.153 kb on - strand, within Rru_A3596 at 4138.163 kb on + strand, within Rru_A3596 at 4138.164 kb on - strand, within Rru_A3596
Per-strain Table
Position Strand Gene LocusTag Fraction Succinate (C)_noKan remove 4,135,434 + +0.0 4,135,434 + +0.1 4,135,944 + -0.1 4,135,944 + +0.0 4,135,944 + +0.6 4,135,944 + -0.1 4,135,944 + -0.0 4,135,945 - -1.0 4,135,945 - -0.1 4,135,945 - +0.1 4,135,945 - -0.4 4,135,958 + +0.5 4,135,959 - -0.4 4,136,105 + Rru_A3593 0.32 -0.4 4,136,238 - Rru_A3593 0.70 -2.4 4,136,419 - -0.6 4,136,449 + -2.0 4,136,473 + +1.2 4,136,474 - -2.4 4,136,475 + -3.8 4,136,557 + Rru_A3594 0.18 +0.2 4,136,557 + Rru_A3594 0.18 -0.4 4,136,627 - Rru_A3594 0.28 -4.7 4,137,060 + Rru_A3594 0.85 -2.2 4,137,929 - +1.2 4,138,148 + Rru_A3596 0.26 -2.8 4,138,149 - Rru_A3596 0.26 -3.0 4,138,149 - Rru_A3596 0.26 -2.5 4,138,152 + Rru_A3596 0.27 -0.4 4,138,153 - Rru_A3596 0.27 -1.6 4,138,153 - Rru_A3596 0.27 +0.2 4,138,153 - Rru_A3596 0.27 -1.1 4,138,153 - Rru_A3596 0.27 +0.0 4,138,153 - Rru_A3596 0.27 -3.8 4,138,153 - Rru_A3596 0.27 -2.4 4,138,163 + Rru_A3596 0.29 -3.9 4,138,164 - Rru_A3596 0.29 -2.8
Or see this region's nucleotide sequence