Experiment: Succinate (C)_noKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A3321 and Rru_A3322 are separated by 166 nucleotides Rru_A3322 and Rru_A3323 are separated by 154 nucleotides Rru_A3323 and Rru_A3324 are separated by 29 nucleotides
Rru_A3321: Rru_A3321 - 3-methyl-2-oxobutanoate hydroxymethyltransferase (NCBI), at 3,830,944 to 3,831,780
_A3321
Rru_A3322: Rru_A3322 - Transcriptional Regulator, Crp/Fnr family (NCBI), at 3,831,947 to 3,832,735
_A3322
Rru_A3323: Rru_A3323 - Cytochrome oxidase maturation protein cbb3-type (NCBI), at 3,832,890 to 3,833,105
_A3323
Rru_A3324: Rru_A3324 - copper-translocating P-type ATPase (NCBI), at 3,833,135 to 3,835,564
_A3324
Position (kb)
3831
3832
3833 Strain fitness (log2 ratio)
-1
0
1 at 3831.904 kb on + strand at 3831.904 kb on + strand at 3832.357 kb on + strand, within Rru_A3322 at 3832.358 kb on - strand, within Rru_A3322 at 3832.359 kb on + strand, within Rru_A3322 at 3832.365 kb on + strand, within Rru_A3322 at 3832.450 kb on - strand, within Rru_A3322 at 3832.485 kb on + strand, within Rru_A3322 at 3832.485 kb on + strand, within Rru_A3322 at 3832.485 kb on + strand, within Rru_A3322 at 3832.485 kb on + strand, within Rru_A3322 at 3832.485 kb on + strand, within Rru_A3322 at 3832.485 kb on + strand, within Rru_A3322 at 3832.486 kb on - strand, within Rru_A3322 at 3832.783 kb on - strand at 3832.882 kb on + strand at 3832.891 kb on + strand at 3833.136 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinate (C)_noKan remove 3,831,904 + +1.0 3,831,904 + +0.2 3,832,357 + Rru_A3322 0.52 -1.3 3,832,358 - Rru_A3322 0.52 -0.8 3,832,359 + Rru_A3322 0.52 -0.0 3,832,365 + Rru_A3322 0.53 -0.9 3,832,450 - Rru_A3322 0.64 -1.1 3,832,485 + Rru_A3322 0.68 +0.6 3,832,485 + Rru_A3322 0.68 -1.0 3,832,485 + Rru_A3322 0.68 -0.9 3,832,485 + Rru_A3322 0.68 -0.6 3,832,485 + Rru_A3322 0.68 -0.0 3,832,485 + Rru_A3322 0.68 +0.0 3,832,486 - Rru_A3322 0.68 -0.3 3,832,783 - -1.1 3,832,882 + -0.3 3,832,891 + -0.6 3,833,136 + +0.2
Or see this region's nucleotide sequence