Experiment: Succinate (C)_noKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0397 and Rru_A0398 overlap by 2 nucleotides Rru_A0398 and Rru_A0399 overlap by 6 nucleotides Rru_A0399 and Rru_A0400 are separated by 148 nucleotides
Rru_A0397: Rru_A0397 - Argininosuccinate lyase (NCBI), at 474,008 to 475,444
_A0397
Rru_A0398: Rru_A0398 - Thiol-disulfide isomerase and thioredoxins-like (NCBI), at 475,443 to 476,114
_A0398
Rru_A0399: Rru_A0399 - Phosphoesterase, PA-phosphatase related (NCBI), at 476,109 to 476,846
_A0399
Rru_A0400: Rru_A0400 - hypothetical protein (NCBI), at 476,995 to 478,437
_A0400
Position (kb) 475
476
477 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 474.454 kb on - strand, within Rru_A0397 at 474.454 kb on - strand, within Rru_A0397 at 474.585 kb on + strand, within Rru_A0397 at 474.585 kb on + strand, within Rru_A0397 at 474.586 kb on - strand, within Rru_A0397 at 474.586 kb on - strand, within Rru_A0397 at 474.889 kb on - strand, within Rru_A0397 at 474.889 kb on - strand, within Rru_A0397 at 475.142 kb on - strand, within Rru_A0397 at 475.142 kb on - strand, within Rru_A0397 at 475.299 kb on - strand, within Rru_A0397 at 475.299 kb on - strand, within Rru_A0397 at 475.301 kb on - strand at 475.897 kb on - strand, within Rru_A0398 at 476.033 kb on + strand, within Rru_A0398 at 476.034 kb on - strand, within Rru_A0398 at 476.034 kb on - strand, within Rru_A0398 at 476.111 kb on - strand at 476.111 kb on - strand at 476.112 kb on + strand at 476.112 kb on + strand at 476.113 kb on - strand at 476.113 kb on - strand at 476.194 kb on + strand, within Rru_A0399 at 476.195 kb on - strand, within Rru_A0399 at 476.221 kb on + strand, within Rru_A0399 at 476.256 kb on + strand, within Rru_A0399 at 476.256 kb on + strand, within Rru_A0399 at 476.560 kb on - strand, within Rru_A0399 at 476.778 kb on - strand at 476.836 kb on - strand at 476.961 kb on + strand at 476.961 kb on + strand at 476.961 kb on + strand at 476.962 kb on - strand at 476.975 kb on + strand at 476.975 kb on + strand at 476.975 kb on + strand at 476.975 kb on + strand at 476.976 kb on - strand at 477.068 kb on + strand at 477.068 kb on + strand at 477.068 kb on + strand at 477.069 kb on - strand at 477.069 kb on - strand at 477.069 kb on - strand at 477.069 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinate (C)_noKan remove 474,454 - Rru_A0397 0.31 -3.4 474,454 - Rru_A0397 0.31 -3.8 474,585 + Rru_A0397 0.40 -2.0 474,585 + Rru_A0397 0.40 -2.0 474,586 - Rru_A0397 0.40 -0.7 474,586 - Rru_A0397 0.40 -6.0 474,889 - Rru_A0397 0.61 -0.1 474,889 - Rru_A0397 0.61 -3.4 475,142 - Rru_A0397 0.79 -3.5 475,142 - Rru_A0397 0.79 -3.5 475,299 - Rru_A0397 0.90 -5.1 475,299 - Rru_A0397 0.90 -5.1 475,301 - -4.2 475,897 - Rru_A0398 0.68 -0.3 476,033 + Rru_A0398 0.88 -0.5 476,034 - Rru_A0398 0.88 -1.0 476,034 - Rru_A0398 0.88 +0.3 476,111 - -0.0 476,111 - +0.1 476,112 + +0.1 476,112 + -0.3 476,113 - +0.0 476,113 - +0.2 476,194 + Rru_A0399 0.12 -0.6 476,195 - Rru_A0399 0.12 -0.2 476,221 + Rru_A0399 0.15 -1.9 476,256 + Rru_A0399 0.20 -1.5 476,256 + Rru_A0399 0.20 -0.8 476,560 - Rru_A0399 0.61 -0.7 476,778 - -0.1 476,836 - -0.4 476,961 + +0.5 476,961 + -0.4 476,961 + +1.5 476,962 - -0.2 476,975 + -0.5 476,975 + -0.1 476,975 + +0.4 476,975 + +0.4 476,976 - +0.6 477,068 + +0.3 477,068 + -0.1 477,068 + -0.3 477,069 - -1.3 477,069 - -0.5 477,069 - +0.0 477,069 - -0.0
Or see this region's nucleotide sequence