Experiment: Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=12hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_19985 and IAI47_19990 are separated by 1 nucleotides IAI47_19990 and IAI47_19995 are separated by 533 nucleotides
IAI47_19985: IAI47_19985 - lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family protein, at 228,169 to 229,461
_19985
IAI47_19990: IAI47_19990 - aspartate aminotransferase family protein, at 229,463 to 231,025
_19990
IAI47_19995: IAI47_19995 - PTS ascorbate transporter subunit IIC, at 231,559 to 232,932
_19995
Position (kb) 229
230
231
232 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 228.549 kb on + strand, within IAI47_19985 at 228.555 kb on + strand, within IAI47_19985 at 228.570 kb on + strand, within IAI47_19985 at 228.571 kb on - strand, within IAI47_19985 at 228.611 kb on + strand, within IAI47_19985 at 228.611 kb on + strand, within IAI47_19985 at 228.611 kb on + strand, within IAI47_19985 at 228.611 kb on + strand, within IAI47_19985 at 228.611 kb on + strand, within IAI47_19985 at 228.611 kb on + strand, within IAI47_19985 at 228.611 kb on + strand, within IAI47_19985 at 228.611 kb on + strand, within IAI47_19985 at 228.611 kb on + strand, within IAI47_19985 at 228.612 kb on - strand, within IAI47_19985 at 228.612 kb on - strand, within IAI47_19985 at 228.612 kb on - strand, within IAI47_19985 at 228.612 kb on - strand, within IAI47_19985 at 228.612 kb on - strand, within IAI47_19985 at 228.612 kb on - strand, within IAI47_19985 at 228.612 kb on - strand, within IAI47_19985 at 228.612 kb on - strand, within IAI47_19985 at 228.612 kb on - strand, within IAI47_19985 at 228.623 kb on + strand, within IAI47_19985 at 228.624 kb on - strand, within IAI47_19985 at 228.637 kb on + strand, within IAI47_19985 at 228.637 kb on + strand, within IAI47_19985 at 228.637 kb on + strand, within IAI47_19985 at 228.638 kb on - strand, within IAI47_19985 at 228.638 kb on - strand, within IAI47_19985 at 228.648 kb on + strand, within IAI47_19985 at 228.648 kb on + strand, within IAI47_19985 at 228.649 kb on - strand, within IAI47_19985 at 228.660 kb on - strand, within IAI47_19985 at 228.719 kb on + strand, within IAI47_19985 at 228.719 kb on + strand, within IAI47_19985 at 228.719 kb on + strand, within IAI47_19985 at 228.720 kb on - strand, within IAI47_19985 at 228.720 kb on - strand, within IAI47_19985 at 228.764 kb on + strand, within IAI47_19985 at 228.765 kb on - strand, within IAI47_19985 at 228.765 kb on - strand, within IAI47_19985 at 228.765 kb on - strand, within IAI47_19985 at 228.776 kb on + strand, within IAI47_19985 at 228.845 kb on + strand, within IAI47_19985 at 228.845 kb on + strand, within IAI47_19985 at 228.846 kb on - strand, within IAI47_19985 at 228.848 kb on + strand, within IAI47_19985 at 228.886 kb on - strand, within IAI47_19985 at 228.926 kb on + strand, within IAI47_19985 at 228.927 kb on - strand, within IAI47_19985 at 229.067 kb on + strand, within IAI47_19985 at 229.068 kb on - strand, within IAI47_19985 at 229.068 kb on - strand, within IAI47_19985 at 229.088 kb on + strand, within IAI47_19985 at 229.089 kb on - strand, within IAI47_19985 at 229.097 kb on + strand, within IAI47_19985 at 229.097 kb on + strand, within IAI47_19985 at 229.097 kb on + strand, within IAI47_19985 at 229.097 kb on + strand, within IAI47_19985 at 229.097 kb on + strand, within IAI47_19985 at 229.097 kb on + strand, within IAI47_19985 at 229.098 kb on - strand, within IAI47_19985 at 229.098 kb on - strand, within IAI47_19985 at 229.125 kb on + strand, within IAI47_19985 at 229.126 kb on - strand, within IAI47_19985 at 229.161 kb on + strand, within IAI47_19985 at 229.162 kb on - strand, within IAI47_19985 at 229.164 kb on - strand, within IAI47_19985 at 229.164 kb on - strand, within IAI47_19985 at 229.164 kb on - strand, within IAI47_19985 at 229.197 kb on + strand, within IAI47_19985 at 229.199 kb on + strand, within IAI47_19985 at 229.200 kb on - strand, within IAI47_19985 at 229.209 kb on - strand, within IAI47_19985 at 229.232 kb on + strand, within IAI47_19985 at 229.233 kb on - strand, within IAI47_19985 at 229.236 kb on + strand, within IAI47_19985 at 229.236 kb on + strand at 229.237 kb on - strand, within IAI47_19985 at 229.248 kb on - strand, within IAI47_19985 at 229.287 kb on + strand, within IAI47_19985 at 229.365 kb on + strand at 229.365 kb on + strand at 229.366 kb on - strand at 229.438 kb on - strand at 229.438 kb on - strand at 229.456 kb on - strand at 229.456 kb on - strand at 229.522 kb on - strand at 229.577 kb on + strand at 229.577 kb on + strand at 229.578 kb on - strand at 229.578 kb on - strand at 229.597 kb on - strand at 229.597 kb on - strand at 229.637 kb on - strand, within IAI47_19990 at 229.649 kb on + strand, within IAI47_19990 at 229.649 kb on + strand, within IAI47_19990 at 229.649 kb on + strand, within IAI47_19990 at 229.650 kb on - strand, within IAI47_19990 at 229.657 kb on + strand, within IAI47_19990 at 229.716 kb on + strand, within IAI47_19990 at 229.782 kb on - strand, within IAI47_19990 at 229.782 kb on - strand, within IAI47_19990 at 229.960 kb on - strand, within IAI47_19990 at 230.050 kb on - strand, within IAI47_19990 at 230.098 kb on + strand, within IAI47_19990 at 230.130 kb on + strand, within IAI47_19990 at 230.131 kb on - strand, within IAI47_19990 at 230.153 kb on + strand, within IAI47_19990 at 230.154 kb on - strand, within IAI47_19990 at 230.160 kb on + strand, within IAI47_19990 at 230.161 kb on - strand, within IAI47_19990 at 230.249 kb on + strand, within IAI47_19990 at 230.249 kb on + strand, within IAI47_19990 at 230.250 kb on - strand, within IAI47_19990 at 230.344 kb on - strand, within IAI47_19990 at 230.401 kb on + strand, within IAI47_19990 at 230.446 kb on + strand, within IAI47_19990 at 230.447 kb on - strand, within IAI47_19990 at 230.715 kb on + strand, within IAI47_19990 at 230.715 kb on + strand, within IAI47_19990 at 230.716 kb on - strand, within IAI47_19990 at 230.719 kb on + strand, within IAI47_19990 at 230.719 kb on + strand, within IAI47_19990 at 230.719 kb on + strand, within IAI47_19990 at 230.719 kb on + strand, within IAI47_19990 at 230.720 kb on - strand, within IAI47_19990 at 230.720 kb on - strand, within IAI47_19990 at 230.751 kb on + strand, within IAI47_19990 at 230.752 kb on - strand, within IAI47_19990 at 230.752 kb on - strand, within IAI47_19990 at 230.868 kb on - strand, within IAI47_19990 at 230.868 kb on - strand, within IAI47_19990 at 231.017 kb on + strand at 231.017 kb on + strand at 231.018 kb on - strand at 231.018 kb on - strand at 231.018 kb on - strand at 231.044 kb on - strand at 231.046 kb on + strand at 231.047 kb on - strand at 231.047 kb on - strand at 231.047 kb on - strand at 231.067 kb on + strand at 231.067 kb on + strand at 231.111 kb on + strand at 231.111 kb on + strand at 231.111 kb on + strand at 231.112 kb on - strand at 231.112 kb on - strand at 231.122 kb on - strand at 231.129 kb on + strand at 231.176 kb on + strand at 231.181 kb on - strand at 231.181 kb on - strand at 231.190 kb on + strand at 231.190 kb on + strand at 231.190 kb on + strand at 231.190 kb on + strand at 231.190 kb on + strand at 231.190 kb on + strand at 231.191 kb on - strand at 231.212 kb on + strand at 231.212 kb on + strand at 231.213 kb on - strand at 231.213 kb on - strand at 231.213 kb on - strand at 231.223 kb on + strand at 231.277 kb on + strand at 231.288 kb on + strand at 231.353 kb on + strand at 231.363 kb on - strand at 231.424 kb on + strand at 231.425 kb on - strand at 231.434 kb on - strand at 231.438 kb on + strand at 231.438 kb on + strand at 231.438 kb on + strand at 231.439 kb on - strand at 231.444 kb on + strand at 231.444 kb on + strand at 231.445 kb on - strand at 231.445 kb on - strand at 231.474 kb on + strand at 231.474 kb on + strand at 231.526 kb on + strand at 231.544 kb on + strand at 231.547 kb on + strand at 231.547 kb on + strand at 231.547 kb on + strand at 231.548 kb on - strand at 231.548 kb on - strand at 231.561 kb on - strand at 231.607 kb on - strand at 231.607 kb on - strand at 231.607 kb on - strand at 231.608 kb on + strand at 231.616 kb on + strand at 231.617 kb on - strand at 231.630 kb on + strand at 231.667 kb on - strand at 231.667 kb on - strand at 231.667 kb on - strand at 231.679 kb on - strand at 231.729 kb on + strand, within IAI47_19995 at 231.729 kb on + strand, within IAI47_19995 at 231.730 kb on - strand, within IAI47_19995 at 231.730 kb on - strand, within IAI47_19995 at 231.783 kb on + strand, within IAI47_19995 at 231.783 kb on + strand, within IAI47_19995 at 231.784 kb on - strand, within IAI47_19995 at 231.784 kb on - strand, within IAI47_19995 at 231.843 kb on + strand, within IAI47_19995 at 231.843 kb on + strand, within IAI47_19995 at 231.850 kb on + strand, within IAI47_19995 at 231.851 kb on - strand, within IAI47_19995 at 231.851 kb on - strand, within IAI47_19995 at 231.901 kb on + strand, within IAI47_19995 at 231.905 kb on - strand, within IAI47_19995 at 231.963 kb on + strand, within IAI47_19995 at 231.964 kb on - strand, within IAI47_19995 at 231.993 kb on - strand, within IAI47_19995
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=12hrs; Outgrowth=LBkan50 remove 228,549 + IAI47_19985 0.29 -1.2 228,555 + IAI47_19985 0.30 -2.2 228,570 + IAI47_19985 0.31 -2.1 228,571 - IAI47_19985 0.31 -0.5 228,611 + IAI47_19985 0.34 +2.1 228,611 + IAI47_19985 0.34 +0.8 228,611 + IAI47_19985 0.34 +0.8 228,611 + IAI47_19985 0.34 -0.5 228,611 + IAI47_19985 0.34 +0.5 228,611 + IAI47_19985 0.34 +1.2 228,611 + IAI47_19985 0.34 +0.2 228,611 + IAI47_19985 0.34 -3.2 228,611 + IAI47_19985 0.34 +0.5 228,612 - IAI47_19985 0.34 +0.4 228,612 - IAI47_19985 0.34 -0.1 228,612 - IAI47_19985 0.34 +0.6 228,612 - IAI47_19985 0.34 -0.3 228,612 - IAI47_19985 0.34 -0.5 228,612 - IAI47_19985 0.34 +0.3 228,612 - IAI47_19985 0.34 +0.2 228,612 - IAI47_19985 0.34 +0.9 228,612 - IAI47_19985 0.34 +0.6 228,623 + IAI47_19985 0.35 -0.9 228,624 - IAI47_19985 0.35 -0.9 228,637 + IAI47_19985 0.36 -1.1 228,637 + IAI47_19985 0.36 -0.3 228,637 + IAI47_19985 0.36 -1.6 228,638 - IAI47_19985 0.36 +0.3 228,638 - IAI47_19985 0.36 -1.1 228,648 + IAI47_19985 0.37 +0.2 228,648 + IAI47_19985 0.37 -1.1 228,649 - IAI47_19985 0.37 +0.8 228,660 - IAI47_19985 0.38 +1.0 228,719 + IAI47_19985 0.43 +0.9 228,719 + IAI47_19985 0.43 +0.5 228,719 + IAI47_19985 0.43 +0.2 228,720 - IAI47_19985 0.43 +0.1 228,720 - IAI47_19985 0.43 +1.6 228,764 + IAI47_19985 0.46 -0.1 228,765 - IAI47_19985 0.46 -1.5 228,765 - IAI47_19985 0.46 +0.1 228,765 - IAI47_19985 0.46 +0.3 228,776 + IAI47_19985 0.47 +1.2 228,845 + IAI47_19985 0.52 -1.6 228,845 + IAI47_19985 0.52 +0.0 228,846 - IAI47_19985 0.52 -0.6 228,848 + IAI47_19985 0.53 -1.2 228,886 - IAI47_19985 0.55 -0.7 228,926 + IAI47_19985 0.59 -0.6 228,927 - IAI47_19985 0.59 +2.5 229,067 + IAI47_19985 0.69 -0.4 229,068 - IAI47_19985 0.70 -0.2 229,068 - IAI47_19985 0.70 +1.2 229,088 + IAI47_19985 0.71 -0.4 229,089 - IAI47_19985 0.71 +0.7 229,097 + IAI47_19985 0.72 -0.4 229,097 + IAI47_19985 0.72 -0.9 229,097 + IAI47_19985 0.72 -0.3 229,097 + IAI47_19985 0.72 +0.9 229,097 + IAI47_19985 0.72 +0.5 229,097 + IAI47_19985 0.72 +0.6 229,098 - IAI47_19985 0.72 +0.2 229,098 - IAI47_19985 0.72 +1.1 229,125 + IAI47_19985 0.74 +0.8 229,126 - IAI47_19985 0.74 +0.8 229,161 + IAI47_19985 0.77 -0.8 229,162 - IAI47_19985 0.77 +0.6 229,164 - IAI47_19985 0.77 +0.0 229,164 - IAI47_19985 0.77 +0.1 229,164 - IAI47_19985 0.77 +0.2 229,197 + IAI47_19985 0.80 +0.5 229,199 + IAI47_19985 0.80 +0.1 229,200 - IAI47_19985 0.80 +0.8 229,209 - IAI47_19985 0.80 +0.3 229,232 + IAI47_19985 0.82 -1.8 229,233 - IAI47_19985 0.82 +0.3 229,236 + IAI47_19985 0.83 +1.0 229,236 + -0.3 229,237 - IAI47_19985 0.83 -1.2 229,248 - IAI47_19985 0.83 -0.4 229,287 + IAI47_19985 0.86 +0.3 229,365 + -1.1 229,365 + -0.1 229,366 - -0.5 229,438 - +0.2 229,438 - +0.3 229,456 - +0.5 229,456 - +1.0 229,522 - +0.7 229,577 + -0.1 229,577 + +1.2 229,578 - -0.2 229,578 - -0.8 229,597 - +0.2 229,597 - +0.5 229,637 - IAI47_19990 0.11 +1.2 229,649 + IAI47_19990 0.12 +0.3 229,649 + IAI47_19990 0.12 -0.4 229,649 + IAI47_19990 0.12 +1.0 229,650 - IAI47_19990 0.12 +2.4 229,657 + IAI47_19990 0.12 +0.3 229,716 + IAI47_19990 0.16 +0.6 229,782 - IAI47_19990 0.20 -0.0 229,782 - IAI47_19990 0.20 -0.3 229,960 - IAI47_19990 0.32 -1.8 230,050 - IAI47_19990 0.38 -1.3 230,098 + IAI47_19990 0.41 +1.7 230,130 + IAI47_19990 0.43 +0.8 230,131 - IAI47_19990 0.43 +0.4 230,153 + IAI47_19990 0.44 +0.5 230,154 - IAI47_19990 0.44 -0.3 230,160 + IAI47_19990 0.45 +0.3 230,161 - IAI47_19990 0.45 +2.4 230,249 + IAI47_19990 0.50 -0.3 230,249 + IAI47_19990 0.50 -0.3 230,250 - IAI47_19990 0.50 +0.6 230,344 - IAI47_19990 0.56 +0.7 230,401 + IAI47_19990 0.60 +0.1 230,446 + IAI47_19990 0.63 +0.7 230,447 - IAI47_19990 0.63 +2.8 230,715 + IAI47_19990 0.80 +0.2 230,715 + IAI47_19990 0.80 -0.2 230,716 - IAI47_19990 0.80 +1.0 230,719 + IAI47_19990 0.80 -1.0 230,719 + IAI47_19990 0.80 +2.0 230,719 + IAI47_19990 0.80 +0.9 230,719 + IAI47_19990 0.80 +0.2 230,720 - IAI47_19990 0.80 +1.3 230,720 - IAI47_19990 0.80 +0.0 230,751 + IAI47_19990 0.82 +0.4 230,752 - IAI47_19990 0.82 +1.0 230,752 - IAI47_19990 0.82 +2.2 230,868 - IAI47_19990 0.90 -0.3 230,868 - IAI47_19990 0.90 +0.9 231,017 + -1.9 231,017 + +1.2 231,018 - +0.2 231,018 - -0.9 231,018 - -0.9 231,044 - -0.2 231,046 + +2.2 231,047 - +0.2 231,047 - -0.1 231,047 - +0.0 231,067 + -0.7 231,067 + -0.0 231,111 + +0.7 231,111 + +0.6 231,111 + -0.9 231,112 - +0.7 231,112 - +0.8 231,122 - +0.6 231,129 + +0.2 231,176 + +0.6 231,181 - +0.8 231,181 - +0.4 231,190 + +0.4 231,190 + -0.2 231,190 + +0.8 231,190 + -0.6 231,190 + +0.4 231,190 + -1.0 231,191 - -0.7 231,212 + +0.1 231,212 + -1.0 231,213 - +1.6 231,213 - +1.8 231,213 - -0.9 231,223 + +1.1 231,277 + -0.3 231,288 + -1.6 231,353 + +0.8 231,363 - +0.1 231,424 + +0.8 231,425 - +0.4 231,434 - -1.9 231,438 + -1.3 231,438 + +0.2 231,438 + +0.8 231,439 - -0.2 231,444 + +0.0 231,444 + +0.7 231,445 - -0.1 231,445 - +1.1 231,474 + +0.5 231,474 + +1.5 231,526 + -0.2 231,544 + -1.1 231,547 + -0.8 231,547 + -1.1 231,547 + +0.3 231,548 - +1.0 231,548 - -0.2 231,561 - -0.5 231,607 - +0.2 231,607 - -0.2 231,607 - +0.6 231,608 + +0.2 231,616 + +0.3 231,617 - +0.5 231,630 + -0.6 231,667 - -0.2 231,667 - +2.8 231,667 - +0.2 231,679 - +0.4 231,729 + IAI47_19995 0.12 -0.4 231,729 + IAI47_19995 0.12 -1.4 231,730 - IAI47_19995 0.12 +0.9 231,730 - IAI47_19995 0.12 -1.1 231,783 + IAI47_19995 0.16 -0.9 231,783 + IAI47_19995 0.16 +1.4 231,784 - IAI47_19995 0.16 +2.2 231,784 - IAI47_19995 0.16 +1.0 231,843 + IAI47_19995 0.21 +1.0 231,843 + IAI47_19995 0.21 -0.7 231,850 + IAI47_19995 0.21 -1.5 231,851 - IAI47_19995 0.21 +0.9 231,851 - IAI47_19995 0.21 +0.5 231,901 + IAI47_19995 0.25 -0.2 231,905 - IAI47_19995 0.25 -1.0 231,963 + IAI47_19995 0.29 -0.6 231,964 - IAI47_19995 0.29 +0.2 231,993 - IAI47_19995 0.32 -0.0
Or see this region's nucleotide sequence