Experiment: MoLS4 with L-Lysine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thrS and tRNA-Val are separated by 85 nucleotides tRNA-Val and DVU2539 are separated by 24 nucleotides DVU2539 and DVU2540 are separated by 5 nucleotides DVU2540 and DVU2541 are separated by 7 nucleotides
DVU2538: thrS - threonyl-tRNA synthetase (TIGR), at 2,649,245 to 2,651,179
thrS
DVU_tRNA-Val-2: tRNA-Val - tRNA with anticodon GAC for Val (VIMSS-AUTO), at 2,651,265 to 2,651,339
tRNA-Val
DVU2539: DVU2539 - hypothetical protein (TIGR), at 2,651,364 to 2,651,486
DVU2539
DVU2540: DVU2540 - lyase, putative (TIGR), at 2,651,492 to 2,652,634
DVU2540
DVU2541: DVU2541 - CoA-substrate-specific enzyme activase, putative (TIGR), at 2,652,642 to 2,653,451
DVU2541
Position (kb)
2651
2652
2653 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2651.462 kb on + strand, within DVU2539 at 2651.479 kb on + strand at 2651.529 kb on + strand at 2651.551 kb on + strand at 2651.559 kb on - strand at 2651.595 kb on - strand at 2651.611 kb on + strand, within DVU2540 at 2651.617 kb on - strand, within DVU2540 at 2651.634 kb on - strand, within DVU2540 at 2651.634 kb on - strand, within DVU2540 at 2651.720 kb on - strand, within DVU2540 at 2651.732 kb on - strand, within DVU2540 at 2651.742 kb on + strand, within DVU2540 at 2651.778 kb on + strand, within DVU2540 at 2651.811 kb on - strand, within DVU2540 at 2651.829 kb on - strand, within DVU2540 at 2651.843 kb on + strand, within DVU2540 at 2651.870 kb on - strand, within DVU2540 at 2651.920 kb on - strand, within DVU2540 at 2651.946 kb on - strand, within DVU2540 at 2651.948 kb on - strand, within DVU2540 at 2651.957 kb on + strand, within DVU2540 at 2652.009 kb on - strand, within DVU2540 at 2652.091 kb on - strand, within DVU2540 at 2652.139 kb on - strand, within DVU2540 at 2652.165 kb on - strand, within DVU2540 at 2652.186 kb on - strand, within DVU2540 at 2652.202 kb on + strand, within DVU2540 at 2652.225 kb on + strand, within DVU2540 at 2652.225 kb on + strand, within DVU2540 at 2652.241 kb on + strand, within DVU2540 at 2652.283 kb on - strand, within DVU2540 at 2652.487 kb on + strand, within DVU2540 at 2652.519 kb on + strand, within DVU2540 at 2652.546 kb on + strand at 2652.704 kb on - strand at 2652.745 kb on + strand, within DVU2541 at 2652.745 kb on + strand, within DVU2541 at 2652.847 kb on + strand, within DVU2541 at 2652.869 kb on + strand, within DVU2541 at 2652.900 kb on - strand, within DVU2541 at 2652.928 kb on + strand, within DVU2541 at 2652.936 kb on - strand, within DVU2541 at 2652.964 kb on + strand, within DVU2541 at 2652.977 kb on + strand, within DVU2541 at 2653.000 kb on - strand, within DVU2541 at 2653.010 kb on + strand, within DVU2541 at 2653.035 kb on + strand, within DVU2541 at 2653.043 kb on + strand, within DVU2541 at 2653.092 kb on + strand, within DVU2541 at 2653.102 kb on + strand, within DVU2541 at 2653.102 kb on + strand, within DVU2541 at 2653.102 kb on + strand, within DVU2541 at 2653.107 kb on + strand, within DVU2541 at 2653.126 kb on + strand, within DVU2541 at 2653.134 kb on + strand, within DVU2541 at 2653.203 kb on + strand, within DVU2541 at 2653.270 kb on + strand, within DVU2541 at 2653.320 kb on - strand, within DVU2541 at 2653.376 kb on + strand at 2653.377 kb on - strand at 2653.384 kb on - strand at 2653.388 kb on + strand at 2653.453 kb on + strand at 2653.470 kb on + strand at 2653.478 kb on - strand at 2653.478 kb on - strand at 2653.559 kb on + strand at 2653.559 kb on + strand at 2653.567 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Lysine (N) remove 2,651,462 + DVU2539 0.80 +0.3 2,651,479 + -1.4 2,651,529 + -0.7 2,651,551 + +0.4 2,651,559 - -0.4 2,651,595 - -0.6 2,651,611 + DVU2540 0.10 +0.5 2,651,617 - DVU2540 0.11 -1.1 2,651,634 - DVU2540 0.12 -0.3 2,651,634 - DVU2540 0.12 -1.1 2,651,720 - DVU2540 0.20 +0.2 2,651,732 - DVU2540 0.21 -1.0 2,651,742 + DVU2540 0.22 +0.0 2,651,778 + DVU2540 0.25 -1.3 2,651,811 - DVU2540 0.28 -0.2 2,651,829 - DVU2540 0.29 -0.2 2,651,843 + DVU2540 0.31 -1.4 2,651,870 - DVU2540 0.33 -1.5 2,651,920 - DVU2540 0.37 -0.3 2,651,946 - DVU2540 0.40 +0.7 2,651,948 - DVU2540 0.40 -0.4 2,651,957 + DVU2540 0.41 -1.1 2,652,009 - DVU2540 0.45 +0.2 2,652,091 - DVU2540 0.52 -0.1 2,652,139 - DVU2540 0.57 -1.3 2,652,165 - DVU2540 0.59 -1.0 2,652,186 - DVU2540 0.61 -1.2 2,652,202 + DVU2540 0.62 +0.0 2,652,225 + DVU2540 0.64 -1.0 2,652,225 + DVU2540 0.64 -0.9 2,652,241 + DVU2540 0.66 +0.6 2,652,283 - DVU2540 0.69 -1.5 2,652,487 + DVU2540 0.87 -0.9 2,652,519 + DVU2540 0.90 -3.1 2,652,546 + -0.5 2,652,704 - +0.1 2,652,745 + DVU2541 0.13 +0.1 2,652,745 + DVU2541 0.13 -0.0 2,652,847 + DVU2541 0.25 -0.5 2,652,869 + DVU2541 0.28 -1.6 2,652,900 - DVU2541 0.32 -0.8 2,652,928 + DVU2541 0.35 -0.1 2,652,936 - DVU2541 0.36 -0.1 2,652,964 + DVU2541 0.40 -2.5 2,652,977 + DVU2541 0.41 -1.0 2,653,000 - DVU2541 0.44 +0.0 2,653,010 + DVU2541 0.45 -0.2 2,653,035 + DVU2541 0.49 +1.2 2,653,043 + DVU2541 0.50 +0.0 2,653,092 + DVU2541 0.56 -3.7 2,653,102 + DVU2541 0.57 -1.2 2,653,102 + DVU2541 0.57 +0.0 2,653,102 + DVU2541 0.57 -0.5 2,653,107 + DVU2541 0.57 -0.0 2,653,126 + DVU2541 0.60 +1.3 2,653,134 + DVU2541 0.61 -1.6 2,653,203 + DVU2541 0.69 -0.4 2,653,270 + DVU2541 0.78 -0.7 2,653,320 - DVU2541 0.84 -0.5 2,653,376 + -0.1 2,653,377 - -0.1 2,653,384 - -1.5 2,653,388 + +0.1 2,653,453 + -0.1 2,653,470 + -0.5 2,653,478 - +0.7 2,653,478 - -0.2 2,653,559 + -0.2 2,653,559 + -1.8 2,653,567 - -2.7
Or see this region's nucleotide sequence