Experiment: MoLS4 with L-Lysine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdxJ and acpS are separated by 10 nucleotides acpS and DVU1910 are separated by 121 nucleotides DVU1910 and DVU1911 overlap by 8 nucleotides DVU1911 and DVU1912 are separated by 12 nucleotides
DVU1908: pdxJ - pyridoxal phosphate biosynthetic protein PdxJ (TIGR), at 1,983,729 to 1,984,454
pdxJ
DVU1909: acpS - holo-(acyl-carrier-protein) synthase (TIGR), at 1,984,465 to 1,984,839
acpS
DVU1910: DVU1910 - YjeF-related protein (TIGR), at 1,984,961 to 1,986,685
DVU1910
DVU1911: DVU1911 - CBS domain protein (TIGR), at 1,986,678 to 1,987,130
DVU1911
DVU1912: DVU1912 - conserved hypothetical protein TIGR00150 (TIGR), at 1,987,143 to 1,987,631
DVU1912
Position (kb)
1984
1985
1986
1987 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1984.186 kb on + strand, within pdxJ at 1984.255 kb on + strand, within pdxJ at 1984.909 kb on + strand at 1984.939 kb on - strand at 1984.947 kb on - strand at 1984.947 kb on - strand at 1985.031 kb on + strand at 1985.074 kb on - strand at 1985.074 kb on - strand at 1985.189 kb on - strand, within DVU1910 at 1985.217 kb on - strand, within DVU1910 at 1985.266 kb on + strand, within DVU1910 at 1985.284 kb on - strand, within DVU1910 at 1985.339 kb on - strand, within DVU1910 at 1985.407 kb on - strand, within DVU1910 at 1985.425 kb on + strand, within DVU1910 at 1985.425 kb on + strand, within DVU1910 at 1985.425 kb on + strand, within DVU1910 at 1985.425 kb on + strand, within DVU1910 at 1985.425 kb on + strand, within DVU1910 at 1985.425 kb on + strand, within DVU1910 at 1985.430 kb on - strand, within DVU1910 at 1985.433 kb on - strand, within DVU1910 at 1985.433 kb on - strand, within DVU1910 at 1985.435 kb on + strand, within DVU1910 at 1985.435 kb on + strand, within DVU1910 at 1985.443 kb on - strand, within DVU1910 at 1985.498 kb on + strand, within DVU1910 at 1985.540 kb on + strand, within DVU1910 at 1985.545 kb on + strand, within DVU1910 at 1985.566 kb on + strand, within DVU1910 at 1985.573 kb on + strand, within DVU1910 at 1985.574 kb on - strand, within DVU1910 at 1985.610 kb on + strand, within DVU1910 at 1985.618 kb on - strand, within DVU1910 at 1985.664 kb on + strand, within DVU1910 at 1985.700 kb on - strand, within DVU1910 at 1985.757 kb on + strand, within DVU1910 at 1985.762 kb on + strand, within DVU1910 at 1985.762 kb on + strand, within DVU1910 at 1985.770 kb on - strand, within DVU1910 at 1985.770 kb on - strand, within DVU1910 at 1985.788 kb on - strand, within DVU1910 at 1985.838 kb on + strand, within DVU1910 at 1985.838 kb on + strand, within DVU1910 at 1985.845 kb on + strand, within DVU1910 at 1985.845 kb on + strand, within DVU1910 at 1985.897 kb on - strand, within DVU1910 at 1985.913 kb on + strand, within DVU1910 at 1985.918 kb on + strand, within DVU1910 at 1985.920 kb on + strand, within DVU1910 at 1985.962 kb on - strand, within DVU1910 at 1985.991 kb on + strand, within DVU1910 at 1986.035 kb on + strand, within DVU1910 at 1986.036 kb on + strand, within DVU1910 at 1986.070 kb on + strand, within DVU1910 at 1986.109 kb on + strand, within DVU1910 at 1986.114 kb on + strand, within DVU1910 at 1986.122 kb on - strand, within DVU1910 at 1986.152 kb on + strand, within DVU1910 at 1986.171 kb on + strand, within DVU1910 at 1986.188 kb on + strand, within DVU1910 at 1986.227 kb on - strand, within DVU1910 at 1986.314 kb on + strand, within DVU1910 at 1986.383 kb on - strand, within DVU1910 at 1986.388 kb on + strand, within DVU1910 at 1986.388 kb on - strand, within DVU1910 at 1986.398 kb on - strand, within DVU1910 at 1986.418 kb on + strand, within DVU1910 at 1986.426 kb on - strand, within DVU1910 at 1986.429 kb on + strand, within DVU1910 at 1986.506 kb on + strand, within DVU1910 at 1986.574 kb on - strand at 1986.647 kb on + strand at 1986.655 kb on - strand at 1987.614 kb on - strand at 1987.616 kb on - strand at 1987.625 kb on + strand at 1987.625 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Lysine (N) remove 1,984,186 + pdxJ DVU1908 0.63 -0.1 1,984,255 + pdxJ DVU1908 0.72 -0.1 1,984,909 + +0.8 1,984,939 - -0.8 1,984,947 - -0.2 1,984,947 - -0.7 1,985,031 + -1.0 1,985,074 - -1.3 1,985,074 - +0.5 1,985,189 - DVU1910 0.13 -1.1 1,985,217 - DVU1910 0.15 -0.9 1,985,266 + DVU1910 0.18 -0.7 1,985,284 - DVU1910 0.19 -0.7 1,985,339 - DVU1910 0.22 +0.3 1,985,407 - DVU1910 0.26 -2.1 1,985,425 + DVU1910 0.27 -0.2 1,985,425 + DVU1910 0.27 +0.0 1,985,425 + DVU1910 0.27 +0.1 1,985,425 + DVU1910 0.27 -1.0 1,985,425 + DVU1910 0.27 -0.0 1,985,425 + DVU1910 0.27 -2.3 1,985,430 - DVU1910 0.27 -0.6 1,985,433 - DVU1910 0.27 +0.7 1,985,433 - DVU1910 0.27 -0.7 1,985,435 + DVU1910 0.27 -2.4 1,985,435 + DVU1910 0.27 -0.8 1,985,443 - DVU1910 0.28 -1.1 1,985,498 + DVU1910 0.31 -0.1 1,985,540 + DVU1910 0.34 -0.3 1,985,545 + DVU1910 0.34 -1.2 1,985,566 + DVU1910 0.35 -0.1 1,985,573 + DVU1910 0.35 -1.7 1,985,574 - DVU1910 0.36 -1.3 1,985,610 + DVU1910 0.38 +1.1 1,985,618 - DVU1910 0.38 +0.0 1,985,664 + DVU1910 0.41 -0.7 1,985,700 - DVU1910 0.43 -0.1 1,985,757 + DVU1910 0.46 -0.5 1,985,762 + DVU1910 0.46 +0.0 1,985,762 + DVU1910 0.46 -0.9 1,985,770 - DVU1910 0.47 -0.5 1,985,770 - DVU1910 0.47 -0.7 1,985,788 - DVU1910 0.48 -1.0 1,985,838 + DVU1910 0.51 +0.5 1,985,838 + DVU1910 0.51 +0.3 1,985,845 + DVU1910 0.51 -0.1 1,985,845 + DVU1910 0.51 -0.2 1,985,897 - DVU1910 0.54 -0.0 1,985,913 + DVU1910 0.55 -0.3 1,985,918 + DVU1910 0.55 -0.5 1,985,920 + DVU1910 0.56 +0.1 1,985,962 - DVU1910 0.58 -1.7 1,985,991 + DVU1910 0.60 -1.2 1,986,035 + DVU1910 0.62 -0.4 1,986,036 + DVU1910 0.62 -4.4 1,986,070 + DVU1910 0.64 -1.2 1,986,109 + DVU1910 0.67 -0.7 1,986,114 + DVU1910 0.67 +0.3 1,986,122 - DVU1910 0.67 +0.1 1,986,152 + DVU1910 0.69 +0.1 1,986,171 + DVU1910 0.70 -0.2 1,986,188 + DVU1910 0.71 -0.9 1,986,227 - DVU1910 0.73 -1.1 1,986,314 + DVU1910 0.78 -0.1 1,986,383 - DVU1910 0.82 -0.5 1,986,388 + DVU1910 0.83 -0.2 1,986,388 - DVU1910 0.83 -0.5 1,986,398 - DVU1910 0.83 -1.5 1,986,418 + DVU1910 0.84 +0.1 1,986,426 - DVU1910 0.85 -1.3 1,986,429 + DVU1910 0.85 -0.3 1,986,506 + DVU1910 0.90 -0.2 1,986,574 - +0.2 1,986,647 + -1.2 1,986,655 - -0.7 1,987,614 - -1.3 1,987,616 - -1.2 1,987,625 + -0.7 1,987,625 + +0.2
Or see this region's nucleotide sequence