Experiment: m.b. Cephalothin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_262p00580 and PGA1_262p00590 are separated by 34 nucleotides PGA1_262p00590 and PGA1_262p00600 overlap by 4 nucleotides PGA1_262p00600 and PGA1_262p00610 are separated by 127 nucleotides PGA1_262p00610 and PGA1_262p00620 are separated by 132 nucleotides
PGA1_262p00580: PGA1_262p00580 - putative polysaccharide biosynthesis/export protein, at 66,790 to 68,163
_262p00580
PGA1_262p00590: PGA1_262p00590 - Protein of unknown function (DUF2886)., at 68,198 to 68,857
_262p00590
PGA1_262p00600: PGA1_262p00600 - Bacterial putative lipoprotein (DUF940)., at 68,854 to 70,983
_262p00600
PGA1_262p00610: PGA1_262p00610 - metallophosphoesterase, at 71,111 to 71,839
_262p00610
PGA1_262p00620: PGA1_262p00620 - hypothetical protein, at 71,972 to 72,625
_262p00620
Position (kb) 68
69
70
71 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 67.875 kb on - strand, within PGA1_262p00580 at 67.875 kb on - strand, within PGA1_262p00580 at 67.875 kb on - strand, within PGA1_262p00580 at 68.008 kb on - strand, within PGA1_262p00580 at 68.008 kb on - strand, within PGA1_262p00580 at 68.101 kb on - strand at 68.197 kb on + strand at 68.208 kb on + strand at 68.322 kb on - strand, within PGA1_262p00590 at 68.423 kb on - strand, within PGA1_262p00590 at 68.643 kb on - strand, within PGA1_262p00590 at 68.709 kb on - strand, within PGA1_262p00590 at 68.742 kb on - strand, within PGA1_262p00590 at 68.771 kb on + strand, within PGA1_262p00590 at 68.798 kb on - strand at 68.850 kb on + strand at 68.892 kb on + strand at 68.956 kb on + strand at 68.982 kb on - strand at 69.035 kb on + strand at 69.043 kb on - strand at 69.189 kb on + strand, within PGA1_262p00600 at 69.293 kb on - strand, within PGA1_262p00600 at 69.293 kb on - strand, within PGA1_262p00600 at 69.293 kb on - strand, within PGA1_262p00600 at 69.298 kb on - strand, within PGA1_262p00600 at 69.367 kb on + strand, within PGA1_262p00600 at 69.488 kb on + strand, within PGA1_262p00600 at 69.502 kb on - strand, within PGA1_262p00600 at 69.507 kb on - strand, within PGA1_262p00600 at 69.509 kb on + strand, within PGA1_262p00600 at 69.530 kb on + strand, within PGA1_262p00600 at 69.738 kb on - strand, within PGA1_262p00600 at 69.760 kb on - strand, within PGA1_262p00600 at 69.913 kb on + strand, within PGA1_262p00600 at 69.921 kb on - strand, within PGA1_262p00600 at 70.000 kb on + strand, within PGA1_262p00600 at 70.007 kb on + strand, within PGA1_262p00600 at 70.067 kb on + strand, within PGA1_262p00600 at 70.181 kb on + strand, within PGA1_262p00600 at 70.210 kb on + strand, within PGA1_262p00600 at 70.238 kb on - strand, within PGA1_262p00600 at 70.298 kb on + strand, within PGA1_262p00600 at 70.349 kb on - strand, within PGA1_262p00600 at 70.405 kb on - strand, within PGA1_262p00600 at 70.557 kb on + strand, within PGA1_262p00600 at 70.557 kb on + strand, within PGA1_262p00600 at 70.629 kb on + strand, within PGA1_262p00600 at 70.674 kb on - strand, within PGA1_262p00600 at 70.705 kb on + strand, within PGA1_262p00600 at 70.835 kb on - strand at 70.874 kb on + strand at 71.025 kb on + strand at 71.037 kb on + strand at 71.037 kb on + strand at 71.037 kb on + strand at 71.043 kb on - strand at 71.047 kb on + strand at 71.092 kb on - strand at 71.169 kb on + strand at 71.169 kb on + strand at 71.177 kb on - strand at 71.302 kb on - strand, within PGA1_262p00610 at 71.448 kb on + strand, within PGA1_262p00610 at 71.513 kb on - strand, within PGA1_262p00610 at 71.650 kb on + strand, within PGA1_262p00610
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cephalothin 0.001 mg/ml remove 67,875 - PGA1_262p00580 0.79 -0.8 67,875 - PGA1_262p00580 0.79 -0.2 67,875 - PGA1_262p00580 0.79 -0.4 68,008 - PGA1_262p00580 0.89 -0.2 68,008 - PGA1_262p00580 0.89 -0.2 68,101 - -0.4 68,197 + +1.1 68,208 + +0.2 68,322 - PGA1_262p00590 0.19 -0.6 68,423 - PGA1_262p00590 0.34 -1.9 68,643 - PGA1_262p00590 0.67 +0.1 68,709 - PGA1_262p00590 0.77 -1.4 68,742 - PGA1_262p00590 0.82 +0.4 68,771 + PGA1_262p00590 0.87 -0.8 68,798 - -0.9 68,850 + -0.2 68,892 + -1.4 68,956 + -0.3 68,982 - -1.0 69,035 + +0.3 69,043 - -0.2 69,189 + PGA1_262p00600 0.16 -1.6 69,293 - PGA1_262p00600 0.21 +0.0 69,293 - PGA1_262p00600 0.21 -0.5 69,293 - PGA1_262p00600 0.21 -0.2 69,298 - PGA1_262p00600 0.21 -0.2 69,367 + PGA1_262p00600 0.24 -0.9 69,488 + PGA1_262p00600 0.30 -1.3 69,502 - PGA1_262p00600 0.30 +0.9 69,507 - PGA1_262p00600 0.31 +0.4 69,509 + PGA1_262p00600 0.31 -1.1 69,530 + PGA1_262p00600 0.32 -0.2 69,738 - PGA1_262p00600 0.42 -0.1 69,760 - PGA1_262p00600 0.43 -0.2 69,913 + PGA1_262p00600 0.50 -0.4 69,921 - PGA1_262p00600 0.50 -0.7 70,000 + PGA1_262p00600 0.54 -0.3 70,007 + PGA1_262p00600 0.54 -0.9 70,067 + PGA1_262p00600 0.57 -1.2 70,181 + PGA1_262p00600 0.62 +1.4 70,210 + PGA1_262p00600 0.64 -0.5 70,238 - PGA1_262p00600 0.65 +0.1 70,298 + PGA1_262p00600 0.68 -0.4 70,349 - PGA1_262p00600 0.70 -1.6 70,405 - PGA1_262p00600 0.73 -1.8 70,557 + PGA1_262p00600 0.80 -0.7 70,557 + PGA1_262p00600 0.80 +0.1 70,629 + PGA1_262p00600 0.83 -0.8 70,674 - PGA1_262p00600 0.85 -1.2 70,705 + PGA1_262p00600 0.87 -0.4 70,835 - -1.9 70,874 + -0.6 71,025 + +0.3 71,037 + -1.5 71,037 + -0.7 71,037 + +1.0 71,043 - -0.1 71,047 + -2.0 71,092 - +1.2 71,169 + -0.5 71,169 + -0.0 71,177 - -0.6 71,302 - PGA1_262p00610 0.26 -0.5 71,448 + PGA1_262p00610 0.46 -2.8 71,513 - PGA1_262p00610 0.55 +1.0 71,650 + PGA1_262p00610 0.74 -0.1
Or see this region's nucleotide sequence