Experiment: MoLS4 with L-Lysine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2524 and hynB-2 are separated by 56 nucleotides hynB-2 and hynA-2 are separated by 12 nucleotides hynA-2 and DVU2527 are separated by 142 nucleotides DVU2527 and DVU2528 are separated by 74 nucleotides DVU2528 and pgk are separated by 80 nucleotides
DVU2524: DVU2524 - cytochrome c3, putative (TIGR), at 2,635,568 to 2,636,065
DVU2524
DVU2525: hynB-2 - periplasmic [NiFe] hydrogenase, small subunit, isozyme 2 (TIGR), at 2,636,122 to 2,637,036
hynB-2
DVU2526: hynA-2 - periplasmic [NiFe] hydrogenase, large subunit, isozyme 2 (TIGR), at 2,637,049 to 2,638,698
hynA-2
DVU2527: DVU2527 - transcriptional regulator, putative (TIGR), at 2,638,841 to 2,639,239
DVU2527
DVU2528: DVU2528 - hypothetical protein (TIGR), at 2,639,314 to 2,639,511
DVU2528
DVU2529: pgk - phosphoglycerate kinase (TIGR), at 2,639,592 to 2,640,773
pgk
Position (kb)
2637
2638
2639 Strain fitness (log2 ratio)
-2
-1
0
1 at 2636.067 kb on - strand at 2636.106 kb on + strand at 2636.106 kb on - strand at 2636.137 kb on + strand at 2636.239 kb on + strand, within hynB-2 at 2636.242 kb on - strand, within hynB-2 at 2636.300 kb on + strand, within hynB-2 at 2636.331 kb on + strand, within hynB-2 at 2636.401 kb on - strand, within hynB-2 at 2636.421 kb on + strand, within hynB-2 at 2636.530 kb on - strand, within hynB-2 at 2636.546 kb on + strand, within hynB-2 at 2636.626 kb on - strand, within hynB-2 at 2636.679 kb on + strand, within hynB-2 at 2636.682 kb on + strand, within hynB-2 at 2636.714 kb on - strand, within hynB-2 at 2636.714 kb on - strand, within hynB-2 at 2636.720 kb on - strand, within hynB-2 at 2636.752 kb on + strand, within hynB-2 at 2636.768 kb on + strand, within hynB-2 at 2636.772 kb on - strand, within hynB-2 at 2636.796 kb on + strand, within hynB-2 at 2636.850 kb on - strand, within hynB-2 at 2636.890 kb on + strand, within hynB-2 at 2636.906 kb on - strand, within hynB-2 at 2636.949 kb on + strand at 2637.030 kb on + strand at 2637.038 kb on - strand at 2637.054 kb on + strand at 2637.077 kb on + strand at 2637.080 kb on + strand at 2637.080 kb on + strand at 2637.080 kb on + strand at 2637.080 kb on + strand at 2637.088 kb on - strand at 2637.088 kb on - strand at 2637.088 kb on - strand at 2637.111 kb on + strand at 2637.167 kb on - strand at 2637.227 kb on + strand, within hynA-2 at 2637.228 kb on - strand, within hynA-2 at 2637.233 kb on - strand, within hynA-2 at 2637.289 kb on + strand, within hynA-2 at 2637.312 kb on + strand, within hynA-2 at 2637.441 kb on + strand, within hynA-2 at 2637.617 kb on + strand, within hynA-2 at 2637.638 kb on + strand, within hynA-2 at 2637.675 kb on + strand, within hynA-2 at 2637.675 kb on + strand, within hynA-2 at 2637.708 kb on + strand, within hynA-2 at 2637.758 kb on - strand, within hynA-2 at 2637.978 kb on + strand, within hynA-2 at 2638.000 kb on - strand, within hynA-2 at 2638.052 kb on - strand, within hynA-2 at 2638.116 kb on + strand, within hynA-2 at 2638.353 kb on + strand, within hynA-2 at 2638.353 kb on + strand, within hynA-2 at 2638.353 kb on + strand, within hynA-2 at 2638.361 kb on - strand, within hynA-2 at 2638.361 kb on - strand, within hynA-2 at 2638.419 kb on + strand, within hynA-2 at 2638.444 kb on + strand, within hynA-2 at 2638.574 kb on + strand at 2638.574 kb on + strand at 2638.574 kb on + strand at 2638.574 kb on + strand at 2638.579 kb on + strand at 2638.688 kb on - strand at 2638.714 kb on + strand at 2639.345 kb on + strand, within DVU2528 at 2639.467 kb on - strand, within DVU2528 at 2639.521 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Lysine (N) remove 2,636,067 - +0.4 2,636,106 + -0.1 2,636,106 - +0.1 2,636,137 + +0.1 2,636,239 + hynB-2 DVU2525 0.13 -0.4 2,636,242 - hynB-2 DVU2525 0.13 -0.0 2,636,300 + hynB-2 DVU2525 0.19 +0.5 2,636,331 + hynB-2 DVU2525 0.23 -0.2 2,636,401 - hynB-2 DVU2525 0.30 +0.4 2,636,421 + hynB-2 DVU2525 0.33 -1.6 2,636,530 - hynB-2 DVU2525 0.45 +0.5 2,636,546 + hynB-2 DVU2525 0.46 +0.0 2,636,626 - hynB-2 DVU2525 0.55 +0.1 2,636,679 + hynB-2 DVU2525 0.61 +0.6 2,636,682 + hynB-2 DVU2525 0.61 +0.3 2,636,714 - hynB-2 DVU2525 0.65 -1.1 2,636,714 - hynB-2 DVU2525 0.65 -0.1 2,636,720 - hynB-2 DVU2525 0.65 -1.9 2,636,752 + hynB-2 DVU2525 0.69 -0.1 2,636,768 + hynB-2 DVU2525 0.71 -0.4 2,636,772 - hynB-2 DVU2525 0.71 -0.8 2,636,796 + hynB-2 DVU2525 0.74 -0.9 2,636,850 - hynB-2 DVU2525 0.80 +0.5 2,636,890 + hynB-2 DVU2525 0.84 -0.3 2,636,906 - hynB-2 DVU2525 0.86 +0.1 2,636,949 + -0.6 2,637,030 + +0.8 2,637,038 - -0.1 2,637,054 + +0.1 2,637,077 + -0.1 2,637,080 + +0.3 2,637,080 + -0.1 2,637,080 + +0.0 2,637,080 + -0.0 2,637,088 - -0.1 2,637,088 - -0.0 2,637,088 - +0.5 2,637,111 + -0.7 2,637,167 - -0.0 2,637,227 + hynA-2 DVU2526 0.11 -1.1 2,637,228 - hynA-2 DVU2526 0.11 -0.4 2,637,233 - hynA-2 DVU2526 0.11 +0.1 2,637,289 + hynA-2 DVU2526 0.15 -0.1 2,637,312 + hynA-2 DVU2526 0.16 +0.1 2,637,441 + hynA-2 DVU2526 0.24 -1.1 2,637,617 + hynA-2 DVU2526 0.34 -0.2 2,637,638 + hynA-2 DVU2526 0.36 +0.9 2,637,675 + hynA-2 DVU2526 0.38 +0.0 2,637,675 + hynA-2 DVU2526 0.38 -1.1 2,637,708 + hynA-2 DVU2526 0.40 +1.2 2,637,758 - hynA-2 DVU2526 0.43 -0.2 2,637,978 + hynA-2 DVU2526 0.56 -2.0 2,638,000 - hynA-2 DVU2526 0.58 -0.5 2,638,052 - hynA-2 DVU2526 0.61 -0.2 2,638,116 + hynA-2 DVU2526 0.65 +0.0 2,638,353 + hynA-2 DVU2526 0.79 -0.8 2,638,353 + hynA-2 DVU2526 0.79 -0.7 2,638,353 + hynA-2 DVU2526 0.79 -2.6 2,638,361 - hynA-2 DVU2526 0.80 -0.6 2,638,361 - hynA-2 DVU2526 0.80 -0.2 2,638,419 + hynA-2 DVU2526 0.83 -0.7 2,638,444 + hynA-2 DVU2526 0.85 -0.3 2,638,574 + +0.7 2,638,574 + -0.1 2,638,574 + -0.0 2,638,574 + -1.8 2,638,579 + -0.6 2,638,688 - +0.1 2,638,714 + +0.2 2,639,345 + DVU2528 0.16 -0.2 2,639,467 - DVU2528 0.77 -0.9 2,639,521 - -0.1
Or see this region's nucleotide sequence