Experiment: MoLS4 with L-Lysine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hynB-1 and hynA-1 are separated by 51 nucleotides hynA-1 and hupD are separated by 368 nucleotides hupD and hypC are separated by 11 nucleotides
DVU1921: hynB-1 - periplasmic [NiFe] hydrogenase, small subunit, isozyme 1 (TIGR), at 1,994,643 to 1,995,596
hynB-1
DVU1922: hynA-1 - periplasmic [NiFe] hydrogenase, large subunit, isozyme 1 (TIGR), at 1,995,648 to 1,997,348
hynA-1
DVU1923: hupD - hydrogenase expression/formation protein HupD (TIGR), at 1,997,717 to 1,998,214
hupD
DVU1924: hypC - hydrogenase assembly chaperone hypC/hupF (TIGR), at 1,998,226 to 1,998,477
hypC
Position (kb)
1995
1996
1997
1998 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1994.823 kb on + strand, within hynB-1 at 1994.828 kb on + strand, within hynB-1 at 1994.851 kb on + strand, within hynB-1 at 1994.859 kb on - strand, within hynB-1 at 1995.072 kb on + strand, within hynB-1 at 1995.104 kb on + strand, within hynB-1 at 1995.104 kb on + strand, within hynB-1 at 1995.113 kb on - strand, within hynB-1 at 1995.159 kb on + strand, within hynB-1 at 1995.169 kb on + strand, within hynB-1 at 1995.186 kb on - strand, within hynB-1 at 1995.460 kb on - strand, within hynB-1 at 1995.499 kb on - strand, within hynB-1 at 1995.509 kb on + strand at 1995.533 kb on + strand at 1995.541 kb on - strand at 1995.670 kb on - strand at 1995.711 kb on - strand at 1995.737 kb on - strand at 1995.785 kb on + strand at 1995.793 kb on - strand at 1995.793 kb on - strand at 1995.815 kb on + strand at 1995.832 kb on - strand, within hynA-1 at 1995.866 kb on + strand, within hynA-1 at 1995.868 kb on + strand, within hynA-1 at 1995.901 kb on - strand, within hynA-1 at 1996.035 kb on - strand, within hynA-1 at 1996.085 kb on + strand, within hynA-1 at 1996.085 kb on + strand, within hynA-1 at 1996.092 kb on + strand, within hynA-1 at 1996.134 kb on - strand, within hynA-1 at 1996.150 kb on - strand, within hynA-1 at 1996.150 kb on - strand, within hynA-1 at 1996.150 kb on - strand, within hynA-1 at 1996.157 kb on + strand, within hynA-1 at 1996.165 kb on - strand, within hynA-1 at 1996.170 kb on - strand, within hynA-1 at 1996.287 kb on - strand, within hynA-1 at 1996.301 kb on + strand, within hynA-1 at 1996.309 kb on - strand, within hynA-1 at 1996.309 kb on - strand, within hynA-1 at 1996.345 kb on + strand, within hynA-1 at 1996.372 kb on - strand, within hynA-1 at 1996.404 kb on - strand, within hynA-1 at 1996.424 kb on + strand, within hynA-1 at 1996.436 kb on + strand, within hynA-1 at 1996.458 kb on + strand, within hynA-1 at 1996.499 kb on + strand, within hynA-1 at 1996.499 kb on + strand, within hynA-1 at 1996.504 kb on + strand, within hynA-1 at 1996.507 kb on - strand, within hynA-1 at 1996.509 kb on + strand, within hynA-1 at 1996.517 kb on - strand, within hynA-1 at 1996.529 kb on + strand, within hynA-1 at 1996.622 kb on + strand, within hynA-1 at 1996.630 kb on - strand, within hynA-1 at 1996.731 kb on + strand, within hynA-1 at 1996.833 kb on - strand, within hynA-1 at 1996.847 kb on + strand, within hynA-1 at 1996.855 kb on - strand, within hynA-1 at 1996.904 kb on + strand, within hynA-1 at 1996.992 kb on - strand, within hynA-1 at 1997.027 kb on + strand, within hynA-1 at 1997.194 kb on - strand at 1997.269 kb on + strand at 1997.270 kb on - strand at 1997.280 kb on + strand at 1997.295 kb on - strand at 1997.318 kb on - strand at 1997.355 kb on + strand at 1997.355 kb on + strand at 1997.355 kb on + strand at 1997.355 kb on + strand at 1997.355 kb on + strand at 1997.363 kb on - strand at 1997.363 kb on - strand at 1997.363 kb on - strand at 1997.376 kb on - strand at 1997.491 kb on - strand at 1997.554 kb on - strand at 1997.591 kb on - strand at 1997.707 kb on - strand at 1997.709 kb on - strand at 1997.731 kb on + strand at 1997.772 kb on - strand, within hupD at 1997.779 kb on - strand, within hupD at 1997.779 kb on - strand, within hupD at 1997.820 kb on - strand, within hupD at 1997.821 kb on - strand, within hupD at 1997.888 kb on - strand, within hupD at 1997.890 kb on - strand, within hupD at 1997.900 kb on - strand, within hupD at 1997.961 kb on + strand, within hupD at 1997.969 kb on - strand, within hupD at 1998.022 kb on - strand, within hupD at 1998.024 kb on + strand, within hupD at 1998.034 kb on - strand, within hupD at 1998.121 kb on - strand, within hupD
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Lysine (N) remove 1,994,823 + hynB-1 DVU1921 0.19 -0.3 1,994,828 + hynB-1 DVU1921 0.19 -0.3 1,994,851 + hynB-1 DVU1921 0.22 -0.2 1,994,859 - hynB-1 DVU1921 0.23 -0.8 1,995,072 + hynB-1 DVU1921 0.45 +0.2 1,995,104 + hynB-1 DVU1921 0.48 -0.2 1,995,104 + hynB-1 DVU1921 0.48 -2.2 1,995,113 - hynB-1 DVU1921 0.49 -0.0 1,995,159 + hynB-1 DVU1921 0.54 -0.0 1,995,169 + hynB-1 DVU1921 0.55 -0.7 1,995,186 - hynB-1 DVU1921 0.57 +0.3 1,995,460 - hynB-1 DVU1921 0.86 -0.7 1,995,499 - hynB-1 DVU1921 0.90 +0.1 1,995,509 + -0.0 1,995,533 + +0.4 1,995,541 - -0.0 1,995,670 - +0.3 1,995,711 - +0.0 1,995,737 - +0.4 1,995,785 + -0.1 1,995,793 - -2.2 1,995,793 - +0.2 1,995,815 + -0.6 1,995,832 - hynA-1 DVU1922 0.11 -0.8 1,995,866 + hynA-1 DVU1922 0.13 -0.6 1,995,868 + hynA-1 DVU1922 0.13 +0.1 1,995,901 - hynA-1 DVU1922 0.15 -1.2 1,996,035 - hynA-1 DVU1922 0.23 +0.0 1,996,085 + hynA-1 DVU1922 0.26 -0.7 1,996,085 + hynA-1 DVU1922 0.26 -0.1 1,996,092 + hynA-1 DVU1922 0.26 +0.5 1,996,134 - hynA-1 DVU1922 0.29 -0.1 1,996,150 - hynA-1 DVU1922 0.30 -2.3 1,996,150 - hynA-1 DVU1922 0.30 -0.2 1,996,150 - hynA-1 DVU1922 0.30 -0.0 1,996,157 + hynA-1 DVU1922 0.30 -0.8 1,996,165 - hynA-1 DVU1922 0.30 +0.1 1,996,170 - hynA-1 DVU1922 0.31 -0.1 1,996,287 - hynA-1 DVU1922 0.38 -1.3 1,996,301 + hynA-1 DVU1922 0.38 -0.4 1,996,309 - hynA-1 DVU1922 0.39 +0.8 1,996,309 - hynA-1 DVU1922 0.39 -2.2 1,996,345 + hynA-1 DVU1922 0.41 -1.1 1,996,372 - hynA-1 DVU1922 0.43 +0.6 1,996,404 - hynA-1 DVU1922 0.44 +0.2 1,996,424 + hynA-1 DVU1922 0.46 +0.2 1,996,436 + hynA-1 DVU1922 0.46 +0.1 1,996,458 + hynA-1 DVU1922 0.48 -1.2 1,996,499 + hynA-1 DVU1922 0.50 +3.9 1,996,499 + hynA-1 DVU1922 0.50 -0.4 1,996,504 + hynA-1 DVU1922 0.50 -0.7 1,996,507 - hynA-1 DVU1922 0.50 -0.4 1,996,509 + hynA-1 DVU1922 0.51 -1.9 1,996,517 - hynA-1 DVU1922 0.51 -1.4 1,996,529 + hynA-1 DVU1922 0.52 +0.7 1,996,622 + hynA-1 DVU1922 0.57 -0.3 1,996,630 - hynA-1 DVU1922 0.58 +0.1 1,996,731 + hynA-1 DVU1922 0.64 -0.7 1,996,833 - hynA-1 DVU1922 0.70 -0.3 1,996,847 + hynA-1 DVU1922 0.70 +0.0 1,996,855 - hynA-1 DVU1922 0.71 +0.1 1,996,904 + hynA-1 DVU1922 0.74 +0.1 1,996,992 - hynA-1 DVU1922 0.79 -0.7 1,997,027 + hynA-1 DVU1922 0.81 -2.5 1,997,194 - -0.4 1,997,269 + +0.3 1,997,270 - -0.4 1,997,280 + -0.3 1,997,295 - -1.2 1,997,318 - +1.3 1,997,355 + -0.5 1,997,355 + +0.1 1,997,355 + +0.1 1,997,355 + -0.0 1,997,355 + -0.1 1,997,363 - +0.1 1,997,363 - +1.3 1,997,363 - -0.6 1,997,376 - +0.1 1,997,491 - +0.5 1,997,554 - -1.3 1,997,591 - +0.0 1,997,707 - -0.9 1,997,709 - -1.0 1,997,731 + +0.7 1,997,772 - hupD DVU1923 0.11 -0.3 1,997,779 - hupD DVU1923 0.12 +0.1 1,997,779 - hupD DVU1923 0.12 -0.1 1,997,820 - hupD DVU1923 0.21 -0.3 1,997,821 - hupD DVU1923 0.21 -0.5 1,997,888 - hupD DVU1923 0.34 -0.5 1,997,890 - hupD DVU1923 0.35 +0.4 1,997,900 - hupD DVU1923 0.37 -1.1 1,997,961 + hupD DVU1923 0.49 +0.0 1,997,969 - hupD DVU1923 0.51 -0.9 1,998,022 - hupD DVU1923 0.61 +0.1 1,998,024 + hupD DVU1923 0.62 -0.1 1,998,034 - hupD DVU1923 0.64 +0.4 1,998,121 - hupD DVU1923 0.81 -0.2
Or see this region's nucleotide sequence