Experiment: MoLS4 with L-Lysine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hydA and hydB are separated by 11 nucleotides hydB and hydC are separated by 252 nucleotides hydC and gltD are separated by 23 nucleotides
DVU1769: hydA - periplasmic [Fe] hydrogenase, large subunit (TIGR), at 1,832,483 to 1,833,748
hydA
DVU1770: hydB - periplasmic [Fe] hydrogenase, small subunit (TIGR), at 1,833,760 to 1,834,131
hydB
DVU1771: hydC - [Fe] hydrogenase gamma (TIGR), at 1,834,384 to 1,836,204
hydC
DVU1772: gltD - pyridine nucleotide-disulfide oxidoreductase (TIGR), at 1,836,228 to 1,838,249
gltD
Position (kb)
1834
1835
1836
1837 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1833.451 kb on + strand, within hydA at 1833.451 kb on + strand, within hydA at 1833.459 kb on - strand, within hydA at 1833.476 kb on - strand, within hydA at 1833.535 kb on - strand, within hydA at 1833.537 kb on + strand, within hydA at 1833.545 kb on - strand, within hydA at 1833.564 kb on - strand, within hydA at 1833.773 kb on + strand at 1833.960 kb on + strand, within hydB at 1833.960 kb on + strand, within hydB at 1833.968 kb on - strand, within hydB at 1833.968 kb on - strand, within hydB at 1833.978 kb on - strand, within hydB at 1833.983 kb on + strand, within hydB at 1833.986 kb on - strand, within hydB at 1834.034 kb on + strand, within hydB at 1834.044 kb on + strand, within hydB at 1834.044 kb on + strand, within hydB at 1834.044 kb on + strand, within hydB at 1834.052 kb on + strand, within hydB at 1834.052 kb on - strand, within hydB at 1834.121 kb on + strand at 1834.347 kb on + strand at 1834.394 kb on - strand at 1834.445 kb on + strand at 1834.696 kb on - strand, within hydC at 1834.752 kb on - strand, within hydC at 1834.762 kb on - strand, within hydC at 1834.762 kb on - strand, within hydC at 1834.762 kb on - strand, within hydC at 1834.790 kb on + strand, within hydC at 1834.857 kb on + strand, within hydC at 1834.914 kb on + strand, within hydC at 1835.040 kb on + strand, within hydC at 1835.131 kb on - strand, within hydC at 1835.178 kb on + strand, within hydC at 1835.183 kb on + strand, within hydC at 1835.183 kb on + strand, within hydC at 1835.189 kb on + strand, within hydC at 1835.197 kb on - strand, within hydC at 1835.202 kb on + strand, within hydC at 1835.220 kb on - strand, within hydC at 1835.222 kb on - strand, within hydC at 1835.343 kb on - strand, within hydC at 1835.355 kb on + strand, within hydC at 1835.540 kb on - strand, within hydC at 1835.540 kb on - strand, within hydC at 1835.588 kb on + strand, within hydC at 1835.732 kb on - strand, within hydC at 1835.795 kb on - strand, within hydC at 1835.907 kb on + strand, within hydC at 1835.910 kb on - strand, within hydC at 1835.926 kb on - strand, within hydC at 1835.948 kb on + strand, within hydC at 1835.958 kb on + strand, within hydC at 1835.958 kb on + strand, within hydC at 1835.958 kb on + strand, within hydC at 1835.966 kb on - strand, within hydC at 1835.966 kb on - strand, within hydC at 1835.966 kb on - strand, within hydC at 1835.966 kb on - strand, within hydC at 1836.202 kb on + strand at 1836.294 kb on + strand at 1836.317 kb on + strand at 1836.518 kb on - strand, within gltD at 1836.522 kb on - strand, within gltD at 1836.725 kb on - strand, within gltD at 1836.732 kb on + strand, within gltD at 1836.815 kb on - strand, within gltD at 1836.840 kb on + strand, within gltD at 1836.845 kb on + strand, within gltD at 1836.874 kb on - strand, within gltD at 1836.953 kb on + strand, within gltD at 1837.047 kb on + strand, within gltD at 1837.083 kb on + strand, within gltD at 1837.175 kb on + strand, within gltD
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Lysine (N) remove 1,833,451 + hydA DVU1769 0.76 -0.3 1,833,451 + hydA DVU1769 0.76 -0.4 1,833,459 - hydA DVU1769 0.77 +0.1 1,833,476 - hydA DVU1769 0.78 +0.1 1,833,535 - hydA DVU1769 0.83 -0.7 1,833,537 + hydA DVU1769 0.83 -3.4 1,833,545 - hydA DVU1769 0.84 +0.8 1,833,564 - hydA DVU1769 0.85 -0.0 1,833,773 + -0.9 1,833,960 + hydB DVU1770 0.54 -0.7 1,833,960 + hydB DVU1770 0.54 -1.0 1,833,968 - hydB DVU1770 0.56 -0.9 1,833,968 - hydB DVU1770 0.56 +0.3 1,833,978 - hydB DVU1770 0.59 +0.3 1,833,983 + hydB DVU1770 0.60 -0.8 1,833,986 - hydB DVU1770 0.61 -0.9 1,834,034 + hydB DVU1770 0.74 -1.6 1,834,044 + hydB DVU1770 0.76 +0.0 1,834,044 + hydB DVU1770 0.76 +0.3 1,834,044 + hydB DVU1770 0.76 -0.3 1,834,052 + hydB DVU1770 0.78 +0.2 1,834,052 - hydB DVU1770 0.78 -1.8 1,834,121 + -0.7 1,834,347 + -0.2 1,834,394 - +0.2 1,834,445 + -0.4 1,834,696 - hydC DVU1771 0.17 +0.1 1,834,752 - hydC DVU1771 0.20 -1.3 1,834,762 - hydC DVU1771 0.21 -0.0 1,834,762 - hydC DVU1771 0.21 -2.3 1,834,762 - hydC DVU1771 0.21 +0.5 1,834,790 + hydC DVU1771 0.22 -0.4 1,834,857 + hydC DVU1771 0.26 -2.3 1,834,914 + hydC DVU1771 0.29 +0.0 1,835,040 + hydC DVU1771 0.36 -1.6 1,835,131 - hydC DVU1771 0.41 -0.5 1,835,178 + hydC DVU1771 0.44 +2.0 1,835,183 + hydC DVU1771 0.44 +0.3 1,835,183 + hydC DVU1771 0.44 +0.3 1,835,189 + hydC DVU1771 0.44 -0.0 1,835,197 - hydC DVU1771 0.45 -0.0 1,835,202 + hydC DVU1771 0.45 +0.3 1,835,220 - hydC DVU1771 0.46 +0.0 1,835,222 - hydC DVU1771 0.46 +0.7 1,835,343 - hydC DVU1771 0.53 +0.0 1,835,355 + hydC DVU1771 0.53 +0.1 1,835,540 - hydC DVU1771 0.63 -0.7 1,835,540 - hydC DVU1771 0.63 +0.0 1,835,588 + hydC DVU1771 0.66 -0.5 1,835,732 - hydC DVU1771 0.74 +0.1 1,835,795 - hydC DVU1771 0.77 +0.6 1,835,907 + hydC DVU1771 0.84 -0.5 1,835,910 - hydC DVU1771 0.84 +0.2 1,835,926 - hydC DVU1771 0.85 -0.1 1,835,948 + hydC DVU1771 0.86 +0.3 1,835,958 + hydC DVU1771 0.86 +0.2 1,835,958 + hydC DVU1771 0.86 -0.3 1,835,958 + hydC DVU1771 0.86 -1.5 1,835,966 - hydC DVU1771 0.87 +0.1 1,835,966 - hydC DVU1771 0.87 -0.2 1,835,966 - hydC DVU1771 0.87 -2.6 1,835,966 - hydC DVU1771 0.87 -0.7 1,836,202 + -0.1 1,836,294 + +0.6 1,836,317 + -0.3 1,836,518 - gltD DVU1772 0.14 -0.1 1,836,522 - gltD DVU1772 0.15 -0.7 1,836,725 - gltD DVU1772 0.25 -0.3 1,836,732 + gltD DVU1772 0.25 -0.4 1,836,815 - gltD DVU1772 0.29 -1.8 1,836,840 + gltD DVU1772 0.30 -0.2 1,836,845 + gltD DVU1772 0.31 -1.3 1,836,874 - gltD DVU1772 0.32 -2.0 1,836,953 + gltD DVU1772 0.36 -0.7 1,837,047 + gltD DVU1772 0.41 -0.1 1,837,083 + gltD DVU1772 0.42 -0.3 1,837,175 + gltD DVU1772 0.47 -0.4
Or see this region's nucleotide sequence