Experiment: Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=12hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_19665 and IAI47_19670 are separated by 36 nucleotides IAI47_19670 and IAI47_19675 overlap by 4 nucleotides IAI47_19675 and IAI47_19680 are separated by 225 nucleotides IAI47_19680 and IAI47_19685 are separated by 109 nucleotides
IAI47_19665: IAI47_19665 - diguanylate cyclase, at 162,485 to 163,579
_19665
IAI47_19670: IAI47_19670 - type I methionyl aminopeptidase, at 163,616 to 164,401
_19670
IAI47_19675: IAI47_19675 - ParD-like family protein, at 164,398 to 164,643
_19675
IAI47_19680: IAI47_19680 - hypothetical protein, at 164,869 to 165,285
_19680
IAI47_19685: IAI47_19685 - hypothetical protein, at 165,395 to 165,853
_19685
Position (kb) 164
165 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 163.493 kb on + strand at 163.519 kb on + strand at 163.520 kb on - strand at 163.524 kb on + strand at 163.524 kb on + strand at 163.524 kb on + strand at 163.524 kb on + strand at 163.524 kb on + strand at 163.524 kb on + strand at 163.524 kb on + strand at 163.525 kb on - strand at 163.525 kb on - strand at 163.525 kb on - strand at 163.525 kb on - strand at 163.525 kb on - strand at 163.577 kb on + strand at 163.577 kb on + strand at 163.577 kb on + strand at 163.578 kb on - strand at 163.578 kb on - strand at 163.617 kb on + strand at 163.771 kb on + strand, within IAI47_19670 at 163.846 kb on - strand, within IAI47_19670 at 163.872 kb on + strand, within IAI47_19670 at 163.899 kb on + strand, within IAI47_19670 at 163.900 kb on - strand, within IAI47_19670 at 163.964 kb on + strand, within IAI47_19670 at 163.964 kb on + strand, within IAI47_19670 at 163.964 kb on + strand, within IAI47_19670 at 163.965 kb on - strand, within IAI47_19670 at 163.965 kb on - strand, within IAI47_19670 at 163.965 kb on - strand, within IAI47_19670 at 163.965 kb on - strand, within IAI47_19670 at 163.965 kb on - strand, within IAI47_19670 at 163.965 kb on - strand, within IAI47_19670 at 163.965 kb on - strand, within IAI47_19670 at 163.991 kb on - strand, within IAI47_19670 at 163.991 kb on - strand, within IAI47_19670 at 163.993 kb on + strand, within IAI47_19670 at 163.994 kb on - strand, within IAI47_19670 at 164.013 kb on + strand, within IAI47_19670 at 164.013 kb on + strand, within IAI47_19670 at 164.013 kb on + strand, within IAI47_19670 at 164.013 kb on + strand, within IAI47_19670 at 164.014 kb on - strand, within IAI47_19670 at 164.014 kb on - strand, within IAI47_19670 at 164.014 kb on - strand, within IAI47_19670 at 164.014 kb on - strand, within IAI47_19670 at 164.064 kb on + strand, within IAI47_19670 at 164.064 kb on + strand, within IAI47_19670 at 164.064 kb on + strand, within IAI47_19670 at 164.064 kb on + strand, within IAI47_19670 at 164.064 kb on + strand, within IAI47_19670 at 164.064 kb on + strand, within IAI47_19670 at 164.064 kb on + strand, within IAI47_19670 at 164.064 kb on + strand, within IAI47_19670 at 164.065 kb on - strand, within IAI47_19670 at 164.065 kb on - strand, within IAI47_19670 at 164.126 kb on - strand, within IAI47_19670 at 164.137 kb on + strand, within IAI47_19670 at 164.147 kb on - strand, within IAI47_19670 at 164.147 kb on - strand, within IAI47_19670 at 164.147 kb on - strand, within IAI47_19670 at 164.168 kb on + strand, within IAI47_19670 at 164.168 kb on + strand, within IAI47_19670 at 164.168 kb on + strand, within IAI47_19670 at 164.168 kb on + strand, within IAI47_19670 at 164.168 kb on + strand, within IAI47_19670 at 164.169 kb on - strand, within IAI47_19670 at 164.169 kb on - strand, within IAI47_19670 at 164.180 kb on - strand, within IAI47_19670 at 164.203 kb on + strand, within IAI47_19670 at 164.204 kb on - strand, within IAI47_19670 at 164.206 kb on - strand, within IAI47_19670 at 164.240 kb on - strand, within IAI47_19670 at 164.240 kb on - strand, within IAI47_19670 at 164.240 kb on - strand, within IAI47_19670 at 164.269 kb on + strand, within IAI47_19670 at 164.269 kb on + strand, within IAI47_19670 at 164.270 kb on - strand, within IAI47_19670 at 164.327 kb on + strand at 164.384 kb on + strand at 164.385 kb on - strand at 164.385 kb on - strand at 164.390 kb on - strand at 164.390 kb on - strand at 164.442 kb on + strand, within IAI47_19675 at 164.442 kb on + strand, within IAI47_19675 at 164.442 kb on + strand, within IAI47_19675 at 164.442 kb on + strand, within IAI47_19675 at 164.451 kb on + strand, within IAI47_19675 at 164.545 kb on - strand, within IAI47_19675 at 164.643 kb on + strand at 164.643 kb on + strand at 164.643 kb on + strand at 164.643 kb on + strand at 164.643 kb on + strand at 164.643 kb on + strand at 164.643 kb on + strand at 164.644 kb on - strand at 164.644 kb on - strand at 164.644 kb on - strand at 164.655 kb on - strand at 164.676 kb on + strand at 164.783 kb on - strand at 164.783 kb on - strand at 164.802 kb on + strand at 164.803 kb on - strand at 164.816 kb on - strand at 164.828 kb on + strand at 164.829 kb on - strand at 164.837 kb on + strand at 164.919 kb on + strand, within IAI47_19680 at 164.919 kb on + strand, within IAI47_19680 at 164.919 kb on + strand, within IAI47_19680 at 164.919 kb on + strand, within IAI47_19680 at 164.919 kb on + strand, within IAI47_19680 at 164.920 kb on - strand, within IAI47_19680 at 164.920 kb on - strand, within IAI47_19680 at 164.920 kb on - strand, within IAI47_19680 at 164.920 kb on - strand, within IAI47_19680 at 164.943 kb on + strand, within IAI47_19680 at 164.953 kb on - strand, within IAI47_19680 at 164.953 kb on - strand, within IAI47_19680 at 164.976 kb on + strand, within IAI47_19680 at 165.059 kb on + strand, within IAI47_19680 at 165.059 kb on + strand, within IAI47_19680 at 165.059 kb on + strand, within IAI47_19680 at 165.060 kb on - strand, within IAI47_19680 at 165.060 kb on - strand, within IAI47_19680 at 165.084 kb on + strand, within IAI47_19680 at 165.084 kb on + strand, within IAI47_19680 at 165.084 kb on + strand, within IAI47_19680 at 165.084 kb on + strand, within IAI47_19680 at 165.085 kb on + strand, within IAI47_19680 at 165.085 kb on - strand, within IAI47_19680 at 165.085 kb on - strand, within IAI47_19680 at 165.085 kb on - strand, within IAI47_19680 at 165.085 kb on - strand, within IAI47_19680 at 165.085 kb on - strand, within IAI47_19680 at 165.085 kb on - strand, within IAI47_19680 at 165.085 kb on - strand, within IAI47_19680 at 165.089 kb on - strand, within IAI47_19680 at 165.111 kb on - strand, within IAI47_19680 at 165.118 kb on - strand, within IAI47_19680 at 165.147 kb on + strand, within IAI47_19680 at 165.147 kb on + strand, within IAI47_19680 at 165.147 kb on + strand, within IAI47_19680 at 165.147 kb on + strand, within IAI47_19680 at 165.148 kb on - strand, within IAI47_19680 at 165.148 kb on - strand, within IAI47_19680 at 165.148 kb on - strand, within IAI47_19680 at 165.148 kb on - strand, within IAI47_19680 at 165.149 kb on + strand, within IAI47_19680 at 165.149 kb on + strand, within IAI47_19680 at 165.150 kb on - strand, within IAI47_19680 at 165.150 kb on - strand, within IAI47_19680 at 165.150 kb on - strand, within IAI47_19680 at 165.150 kb on - strand, within IAI47_19680 at 165.190 kb on - strand, within IAI47_19680 at 165.201 kb on + strand, within IAI47_19680 at 165.202 kb on - strand, within IAI47_19680 at 165.212 kb on + strand, within IAI47_19680 at 165.212 kb on + strand, within IAI47_19680 at 165.212 kb on + strand, within IAI47_19680 at 165.212 kb on + strand, within IAI47_19680 at 165.212 kb on + strand, within IAI47_19680 at 165.213 kb on - strand, within IAI47_19680 at 165.213 kb on - strand, within IAI47_19680 at 165.216 kb on + strand, within IAI47_19680 at 165.217 kb on - strand, within IAI47_19680 at 165.222 kb on + strand, within IAI47_19680 at 165.222 kb on + strand, within IAI47_19680 at 165.222 kb on + strand, within IAI47_19680 at 165.222 kb on + strand, within IAI47_19680 at 165.222 kb on + strand, within IAI47_19680 at 165.222 kb on + strand, within IAI47_19680 at 165.258 kb on + strand at 165.258 kb on + strand at 165.259 kb on - strand at 165.283 kb on + strand at 165.283 kb on + strand at 165.308 kb on + strand at 165.309 kb on - strand at 165.309 kb on - strand at 165.337 kb on + strand at 165.338 kb on - strand at 165.364 kb on + strand at 165.364 kb on + strand at 165.364 kb on + strand at 165.364 kb on + strand at 165.365 kb on - strand at 165.365 kb on - strand at 165.390 kb on + strand at 165.390 kb on + strand at 165.391 kb on - strand at 165.401 kb on - strand at 165.408 kb on + strand at 165.408 kb on + strand at 165.409 kb on - strand at 165.409 kb on - strand at 165.412 kb on + strand at 165.412 kb on + strand at 165.412 kb on + strand at 165.413 kb on - strand at 165.413 kb on - strand at 165.413 kb on - strand at 165.446 kb on + strand, within IAI47_19685 at 165.446 kb on + strand, within IAI47_19685 at 165.447 kb on - strand, within IAI47_19685 at 165.511 kb on + strand, within IAI47_19685 at 165.512 kb on - strand, within IAI47_19685 at 165.546 kb on + strand, within IAI47_19685 at 165.590 kb on - strand, within IAI47_19685 at 165.609 kb on - strand, within IAI47_19685
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=12hrs; Outgrowth=LBkan50 remove 163,493 + -0.1 163,519 + -0.4 163,520 - -0.4 163,524 + +1.7 163,524 + -1.0 163,524 + +0.1 163,524 + +1.1 163,524 + -0.3 163,524 + +0.8 163,524 + +0.6 163,525 - -0.4 163,525 - -2.6 163,525 - -1.0 163,525 - +0.9 163,525 - -0.7 163,577 + -1.3 163,577 + -1.1 163,577 + +0.1 163,578 - -0.4 163,578 - +0.5 163,617 + +0.1 163,771 + IAI47_19670 0.20 -1.3 163,846 - IAI47_19670 0.29 +2.4 163,872 + IAI47_19670 0.33 +0.8 163,899 + IAI47_19670 0.36 -2.4 163,900 - IAI47_19670 0.36 -0.6 163,964 + IAI47_19670 0.44 -0.4 163,964 + IAI47_19670 0.44 -0.7 163,964 + IAI47_19670 0.44 -0.6 163,965 - IAI47_19670 0.44 -0.1 163,965 - IAI47_19670 0.44 -0.1 163,965 - IAI47_19670 0.44 -1.6 163,965 - IAI47_19670 0.44 +0.3 163,965 - IAI47_19670 0.44 +0.1 163,965 - IAI47_19670 0.44 +0.2 163,965 - IAI47_19670 0.44 +0.8 163,991 - IAI47_19670 0.48 +0.5 163,991 - IAI47_19670 0.48 -0.0 163,993 + IAI47_19670 0.48 -0.0 163,994 - IAI47_19670 0.48 -0.0 164,013 + IAI47_19670 0.51 -1.2 164,013 + IAI47_19670 0.51 +0.3 164,013 + IAI47_19670 0.51 +0.8 164,013 + IAI47_19670 0.51 -0.2 164,014 - IAI47_19670 0.51 +0.5 164,014 - IAI47_19670 0.51 -0.3 164,014 - IAI47_19670 0.51 -0.3 164,014 - IAI47_19670 0.51 +0.1 164,064 + IAI47_19670 0.57 -1.8 164,064 + IAI47_19670 0.57 +1.0 164,064 + IAI47_19670 0.57 +1.6 164,064 + IAI47_19670 0.57 -0.7 164,064 + IAI47_19670 0.57 -1.4 164,064 + IAI47_19670 0.57 -1.0 164,064 + IAI47_19670 0.57 +0.0 164,064 + IAI47_19670 0.57 -0.5 164,065 - IAI47_19670 0.57 -0.1 164,065 - IAI47_19670 0.57 +1.0 164,126 - IAI47_19670 0.65 +1.5 164,137 + IAI47_19670 0.66 +0.2 164,147 - IAI47_19670 0.68 +2.2 164,147 - IAI47_19670 0.68 +1.3 164,147 - IAI47_19670 0.68 +0.8 164,168 + IAI47_19670 0.70 +0.2 164,168 + IAI47_19670 0.70 +0.4 164,168 + IAI47_19670 0.70 +0.2 164,168 + IAI47_19670 0.70 -0.6 164,168 + IAI47_19670 0.70 -0.8 164,169 - IAI47_19670 0.70 +1.6 164,169 - IAI47_19670 0.70 +1.4 164,180 - IAI47_19670 0.72 +1.9 164,203 + IAI47_19670 0.75 -0.9 164,204 - IAI47_19670 0.75 +2.2 164,206 - IAI47_19670 0.75 -0.2 164,240 - IAI47_19670 0.79 +0.3 164,240 - IAI47_19670 0.79 -0.1 164,240 - IAI47_19670 0.79 -1.9 164,269 + IAI47_19670 0.83 -2.3 164,269 + IAI47_19670 0.83 -1.0 164,270 - IAI47_19670 0.83 +0.4 164,327 + -1.4 164,384 + -0.7 164,385 - -2.0 164,385 - +0.4 164,390 - +0.8 164,390 - +1.9 164,442 + IAI47_19675 0.18 -0.9 164,442 + IAI47_19675 0.18 +0.2 164,442 + IAI47_19675 0.18 -1.6 164,442 + IAI47_19675 0.18 -0.8 164,451 + IAI47_19675 0.22 -1.1 164,545 - IAI47_19675 0.60 +0.1 164,643 + -0.9 164,643 + -1.3 164,643 + +0.5 164,643 + +0.2 164,643 + +0.8 164,643 + -0.3 164,643 + -1.2 164,644 - +0.9 164,644 - +1.4 164,644 - +0.0 164,655 - +0.1 164,676 + +0.4 164,783 - -0.7 164,783 - +0.2 164,802 + +1.0 164,803 - -0.3 164,816 - -0.0 164,828 + +0.2 164,829 - +0.8 164,837 + -0.7 164,919 + IAI47_19680 0.12 +0.8 164,919 + IAI47_19680 0.12 -0.0 164,919 + IAI47_19680 0.12 +0.8 164,919 + IAI47_19680 0.12 -1.3 164,919 + IAI47_19680 0.12 -0.1 164,920 - IAI47_19680 0.12 -0.3 164,920 - IAI47_19680 0.12 -1.7 164,920 - IAI47_19680 0.12 -1.2 164,920 - IAI47_19680 0.12 -1.2 164,943 + IAI47_19680 0.18 +1.0 164,953 - IAI47_19680 0.20 -1.1 164,953 - IAI47_19680 0.20 -1.3 164,976 + IAI47_19680 0.26 +0.7 165,059 + IAI47_19680 0.46 +1.0 165,059 + IAI47_19680 0.46 +0.9 165,059 + IAI47_19680 0.46 -1.9 165,060 - IAI47_19680 0.46 -0.3 165,060 - IAI47_19680 0.46 -0.9 165,084 + IAI47_19680 0.52 +0.3 165,084 + IAI47_19680 0.52 +0.7 165,084 + IAI47_19680 0.52 +0.5 165,084 + IAI47_19680 0.52 -1.3 165,085 + IAI47_19680 0.52 +0.2 165,085 - IAI47_19680 0.52 +0.0 165,085 - IAI47_19680 0.52 -2.2 165,085 - IAI47_19680 0.52 +1.0 165,085 - IAI47_19680 0.52 +1.7 165,085 - IAI47_19680 0.52 +0.8 165,085 - IAI47_19680 0.52 +2.8 165,085 - IAI47_19680 0.52 -1.3 165,089 - IAI47_19680 0.53 -0.8 165,111 - IAI47_19680 0.58 +0.1 165,118 - IAI47_19680 0.60 +0.7 165,147 + IAI47_19680 0.67 +1.4 165,147 + IAI47_19680 0.67 +1.8 165,147 + IAI47_19680 0.67 +2.0 165,147 + IAI47_19680 0.67 +0.1 165,148 - IAI47_19680 0.67 -0.5 165,148 - IAI47_19680 0.67 -0.1 165,148 - IAI47_19680 0.67 -1.1 165,148 - IAI47_19680 0.67 -1.3 165,149 + IAI47_19680 0.67 +0.4 165,149 + IAI47_19680 0.67 -0.3 165,150 - IAI47_19680 0.67 +0.2 165,150 - IAI47_19680 0.67 -1.8 165,150 - IAI47_19680 0.67 -0.5 165,150 - IAI47_19680 0.67 +0.4 165,190 - IAI47_19680 0.77 -0.7 165,201 + IAI47_19680 0.80 -0.4 165,202 - IAI47_19680 0.80 +1.2 165,212 + IAI47_19680 0.82 +0.7 165,212 + IAI47_19680 0.82 +0.2 165,212 + IAI47_19680 0.82 +0.5 165,212 + IAI47_19680 0.82 -0.9 165,212 + IAI47_19680 0.82 +0.6 165,213 - IAI47_19680 0.82 +0.5 165,213 - IAI47_19680 0.82 +0.3 165,216 + IAI47_19680 0.83 -1.0 165,217 - IAI47_19680 0.83 -0.2 165,222 + IAI47_19680 0.85 +0.2 165,222 + IAI47_19680 0.85 -0.5 165,222 + IAI47_19680 0.85 +0.1 165,222 + IAI47_19680 0.85 +0.3 165,222 + IAI47_19680 0.85 +1.8 165,222 + IAI47_19680 0.85 -0.2 165,258 + -0.2 165,258 + +0.7 165,259 - +0.7 165,283 + +0.0 165,283 + -0.6 165,308 + -0.5 165,309 - +0.3 165,309 - +0.5 165,337 + -1.6 165,338 - -1.8 165,364 + +0.6 165,364 + -1.5 165,364 + +1.7 165,364 + +1.0 165,365 - +0.8 165,365 - +1.3 165,390 + +0.0 165,390 + -0.4 165,391 - +0.8 165,401 - +0.8 165,408 + +0.6 165,408 + +0.4 165,409 - +0.8 165,409 - +0.1 165,412 + -0.2 165,412 + +0.1 165,412 + +0.7 165,413 - +1.4 165,413 - -0.5 165,413 - +0.7 165,446 + IAI47_19685 0.11 +0.9 165,446 + IAI47_19685 0.11 +0.1 165,447 - IAI47_19685 0.11 +1.2 165,511 + IAI47_19685 0.25 -1.3 165,512 - IAI47_19685 0.25 -1.0 165,546 + IAI47_19685 0.33 +0.7 165,590 - IAI47_19685 0.42 -0.7 165,609 - IAI47_19685 0.47 -1.1
Or see this region's nucleotide sequence