Experiment: Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=12hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_10180 and IAI47_10185 overlap by 8 nucleotides IAI47_10185 and IAI47_10190 are separated by 9 nucleotides
IAI47_10180: IAI47_10180 - PTS sugar transporter subunit IIC, at 2,151,536 to 2,152,903
_10180
IAI47_10185: IAI47_10185 - PTS sugar transporter subunit IIB, at 2,152,896 to 2,153,210
_10185
IAI47_10190: IAI47_10190 - 6-phosphofructokinase, at 2,153,220 to 2,154,200
_10190
Position (kb)
2152
2153
2154 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2151.969 kb on + strand, within IAI47_10180 at 2151.970 kb on - strand, within IAI47_10180 at 2152.080 kb on + strand, within IAI47_10180 at 2152.081 kb on - strand, within IAI47_10180 at 2152.081 kb on - strand, within IAI47_10180 at 2152.120 kb on + strand, within IAI47_10180 at 2152.121 kb on - strand, within IAI47_10180 at 2152.121 kb on - strand, within IAI47_10180 at 2152.128 kb on + strand, within IAI47_10180 at 2152.128 kb on + strand, within IAI47_10180 at 2152.128 kb on + strand, within IAI47_10180 at 2152.128 kb on + strand, within IAI47_10180 at 2152.129 kb on - strand, within IAI47_10180 at 2152.137 kb on + strand, within IAI47_10180 at 2152.137 kb on + strand, within IAI47_10180 at 2152.137 kb on + strand, within IAI47_10180 at 2152.137 kb on + strand, within IAI47_10180 at 2152.137 kb on + strand, within IAI47_10180 at 2152.138 kb on - strand, within IAI47_10180 at 2152.156 kb on - strand, within IAI47_10180 at 2152.200 kb on + strand, within IAI47_10180 at 2152.200 kb on + strand, within IAI47_10180 at 2152.201 kb on - strand, within IAI47_10180 at 2152.201 kb on - strand, within IAI47_10180 at 2152.201 kb on - strand, within IAI47_10180 at 2152.201 kb on - strand, within IAI47_10180 at 2152.233 kb on + strand, within IAI47_10180 at 2152.233 kb on + strand, within IAI47_10180 at 2152.233 kb on + strand, within IAI47_10180 at 2152.233 kb on + strand, within IAI47_10180 at 2152.233 kb on + strand, within IAI47_10180 at 2152.233 kb on + strand, within IAI47_10180 at 2152.234 kb on - strand, within IAI47_10180 at 2152.234 kb on - strand, within IAI47_10180 at 2152.234 kb on - strand, within IAI47_10180 at 2152.234 kb on - strand, within IAI47_10180 at 2152.234 kb on - strand, within IAI47_10180 at 2152.234 kb on - strand, within IAI47_10180 at 2152.234 kb on - strand, within IAI47_10180 at 2152.234 kb on - strand, within IAI47_10180 at 2152.234 kb on - strand, within IAI47_10180 at 2152.234 kb on - strand, within IAI47_10180 at 2152.234 kb on - strand, within IAI47_10180 at 2152.237 kb on + strand, within IAI47_10180 at 2152.237 kb on + strand, within IAI47_10180 at 2152.238 kb on - strand, within IAI47_10180 at 2152.238 kb on - strand, within IAI47_10180 at 2152.252 kb on + strand, within IAI47_10180 at 2152.252 kb on + strand, within IAI47_10180 at 2152.252 kb on + strand, within IAI47_10180 at 2152.252 kb on + strand, within IAI47_10180 at 2152.333 kb on - strand, within IAI47_10180 at 2152.381 kb on + strand, within IAI47_10180 at 2152.381 kb on + strand, within IAI47_10180 at 2152.426 kb on + strand, within IAI47_10180 at 2152.426 kb on + strand, within IAI47_10180 at 2152.426 kb on + strand, within IAI47_10180 at 2152.426 kb on + strand, within IAI47_10180 at 2152.427 kb on - strand, within IAI47_10180 at 2152.517 kb on + strand, within IAI47_10180 at 2152.517 kb on + strand, within IAI47_10180 at 2152.518 kb on - strand, within IAI47_10180 at 2152.545 kb on + strand, within IAI47_10180 at 2152.545 kb on + strand, within IAI47_10180 at 2152.545 kb on + strand at 2152.545 kb on + strand, within IAI47_10180 at 2152.545 kb on + strand, within IAI47_10180 at 2152.545 kb on + strand, within IAI47_10180 at 2152.546 kb on - strand, within IAI47_10180 at 2152.546 kb on - strand, within IAI47_10180 at 2152.546 kb on - strand, within IAI47_10180 at 2152.546 kb on - strand, within IAI47_10180 at 2152.546 kb on - strand, within IAI47_10180 at 2152.546 kb on - strand, within IAI47_10180 at 2152.546 kb on - strand, within IAI47_10180 at 2152.549 kb on + strand, within IAI47_10180 at 2152.549 kb on + strand, within IAI47_10180 at 2152.549 kb on + strand, within IAI47_10180 at 2152.549 kb on + strand, within IAI47_10180 at 2152.549 kb on + strand, within IAI47_10180 at 2152.549 kb on + strand, within IAI47_10180 at 2152.550 kb on - strand, within IAI47_10180 at 2152.550 kb on - strand, within IAI47_10180 at 2152.550 kb on - strand, within IAI47_10180 at 2152.550 kb on - strand, within IAI47_10180 at 2152.576 kb on + strand, within IAI47_10180 at 2152.577 kb on - strand, within IAI47_10180 at 2152.649 kb on - strand, within IAI47_10180 at 2152.656 kb on - strand, within IAI47_10180 at 2152.656 kb on - strand, within IAI47_10180 at 2152.656 kb on - strand, within IAI47_10180 at 2152.656 kb on - strand, within IAI47_10180 at 2152.666 kb on + strand, within IAI47_10180 at 2152.667 kb on - strand, within IAI47_10180 at 2152.667 kb on - strand, within IAI47_10180 at 2152.668 kb on + strand, within IAI47_10180 at 2152.678 kb on + strand, within IAI47_10180 at 2152.703 kb on - strand, within IAI47_10180 at 2152.729 kb on + strand, within IAI47_10180 at 2152.729 kb on + strand, within IAI47_10180 at 2152.729 kb on + strand, within IAI47_10180 at 2152.729 kb on + strand, within IAI47_10180 at 2152.729 kb on + strand, within IAI47_10180 at 2152.730 kb on - strand, within IAI47_10180 at 2152.730 kb on - strand, within IAI47_10180 at 2152.730 kb on - strand, within IAI47_10180 at 2152.730 kb on - strand, within IAI47_10180 at 2152.730 kb on - strand, within IAI47_10180 at 2152.732 kb on + strand, within IAI47_10180 at 2152.732 kb on + strand, within IAI47_10180 at 2152.732 kb on + strand, within IAI47_10180 at 2152.732 kb on + strand, within IAI47_10180 at 2152.751 kb on - strand, within IAI47_10180 at 2152.751 kb on - strand, within IAI47_10180 at 2152.754 kb on + strand, within IAI47_10180 at 2152.775 kb on - strand at 2152.837 kb on + strand at 2152.897 kb on + strand at 2152.982 kb on + strand, within IAI47_10185 at 2152.988 kb on + strand, within IAI47_10185 at 2152.989 kb on - strand, within IAI47_10185 at 2153.000 kb on + strand, within IAI47_10185 at 2153.001 kb on - strand, within IAI47_10185 at 2153.001 kb on - strand, within IAI47_10185 at 2153.029 kb on - strand, within IAI47_10185 at 2153.029 kb on - strand, within IAI47_10185 at 2153.029 kb on - strand, within IAI47_10185 at 2153.063 kb on + strand, within IAI47_10185 at 2153.063 kb on + strand, within IAI47_10185 at 2153.064 kb on - strand, within IAI47_10185 at 2153.073 kb on - strand, within IAI47_10185 at 2153.073 kb on - strand, within IAI47_10185 at 2153.133 kb on + strand, within IAI47_10185 at 2153.133 kb on + strand, within IAI47_10185 at 2153.137 kb on - strand, within IAI47_10185 at 2153.196 kb on + strand at 2153.196 kb on + strand at 2153.226 kb on + strand at 2153.226 kb on + strand at 2153.226 kb on + strand at 2153.227 kb on - strand at 2153.227 kb on - strand at 2153.227 kb on - strand at 2153.227 kb on - strand at 2153.228 kb on + strand at 2153.228 kb on + strand at 2153.229 kb on - strand at 2153.251 kb on + strand at 2153.293 kb on - strand at 2153.293 kb on - strand at 2153.293 kb on - strand at 2153.315 kb on + strand at 2153.331 kb on - strand, within IAI47_10190 at 2153.342 kb on + strand, within IAI47_10190 at 2153.363 kb on + strand, within IAI47_10190 at 2153.364 kb on - strand, within IAI47_10190 at 2153.450 kb on + strand, within IAI47_10190 at 2153.457 kb on - strand, within IAI47_10190 at 2153.457 kb on - strand, within IAI47_10190 at 2153.457 kb on - strand, within IAI47_10190 at 2153.531 kb on - strand, within IAI47_10190 at 2153.531 kb on - strand, within IAI47_10190 at 2153.542 kb on + strand, within IAI47_10190 at 2153.542 kb on + strand, within IAI47_10190 at 2153.543 kb on - strand, within IAI47_10190 at 2153.664 kb on - strand, within IAI47_10190 at 2153.682 kb on + strand, within IAI47_10190 at 2153.706 kb on + strand, within IAI47_10190 at 2153.706 kb on + strand, within IAI47_10190 at 2153.750 kb on + strand, within IAI47_10190 at 2153.751 kb on - strand, within IAI47_10190 at 2153.773 kb on - strand, within IAI47_10190 at 2153.810 kb on - strand, within IAI47_10190 at 2153.844 kb on - strand, within IAI47_10190 at 2153.923 kb on - strand, within IAI47_10190 at 2153.923 kb on - strand, within IAI47_10190 at 2153.923 kb on - strand, within IAI47_10190 at 2153.925 kb on + strand, within IAI47_10190 at 2153.925 kb on + strand, within IAI47_10190 at 2153.925 kb on + strand, within IAI47_10190 at 2153.925 kb on + strand, within IAI47_10190 at 2153.926 kb on - strand, within IAI47_10190 at 2153.926 kb on - strand, within IAI47_10190 at 2153.926 kb on - strand, within IAI47_10190 at 2153.933 kb on + strand, within IAI47_10190 at 2153.933 kb on + strand, within IAI47_10190 at 2153.933 kb on + strand, within IAI47_10190 at 2153.934 kb on - strand, within IAI47_10190 at 2153.934 kb on - strand, within IAI47_10190 at 2153.934 kb on - strand, within IAI47_10190 at 2153.961 kb on + strand, within IAI47_10190 at 2153.961 kb on + strand, within IAI47_10190 at 2153.962 kb on - strand, within IAI47_10190 at 2153.969 kb on - strand, within IAI47_10190 at 2153.969 kb on - strand, within IAI47_10190 at 2153.969 kb on - strand, within IAI47_10190 at 2154.060 kb on - strand, within IAI47_10190 at 2154.071 kb on + strand, within IAI47_10190 at 2154.071 kb on + strand, within IAI47_10190 at 2154.071 kb on + strand, within IAI47_10190 at 2154.072 kb on - strand, within IAI47_10190 at 2154.072 kb on - strand, within IAI47_10190 at 2154.072 kb on - strand, within IAI47_10190 at 2154.105 kb on + strand at 2154.106 kb on - strand at 2154.135 kb on + strand at 2154.135 kb on + strand at 2154.153 kb on - strand at 2154.153 kb on - strand at 2154.157 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=12hrs; Outgrowth=LBkan50 remove 2,151,969 + IAI47_10180 0.32 +1.4 2,151,970 - IAI47_10180 0.32 -1.4 2,152,080 + IAI47_10180 0.40 -0.0 2,152,081 - IAI47_10180 0.40 -0.2 2,152,081 - IAI47_10180 0.40 -0.2 2,152,120 + IAI47_10180 0.43 +0.8 2,152,121 - IAI47_10180 0.43 +1.4 2,152,121 - IAI47_10180 0.43 +1.1 2,152,128 + IAI47_10180 0.43 -1.2 2,152,128 + IAI47_10180 0.43 -1.1 2,152,128 + IAI47_10180 0.43 +0.9 2,152,128 + IAI47_10180 0.43 +1.1 2,152,129 - IAI47_10180 0.43 +0.0 2,152,137 + IAI47_10180 0.44 -0.3 2,152,137 + IAI47_10180 0.44 -0.2 2,152,137 + IAI47_10180 0.44 +1.3 2,152,137 + IAI47_10180 0.44 -0.8 2,152,137 + IAI47_10180 0.44 -1.4 2,152,138 - IAI47_10180 0.44 -0.6 2,152,156 - IAI47_10180 0.45 -0.4 2,152,200 + IAI47_10180 0.49 +0.5 2,152,200 + IAI47_10180 0.49 -0.4 2,152,201 - IAI47_10180 0.49 +1.1 2,152,201 - IAI47_10180 0.49 +0.1 2,152,201 - IAI47_10180 0.49 -1.4 2,152,201 - IAI47_10180 0.49 -0.7 2,152,233 + IAI47_10180 0.51 -1.0 2,152,233 + IAI47_10180 0.51 -2.3 2,152,233 + IAI47_10180 0.51 -2.4 2,152,233 + IAI47_10180 0.51 +2.0 2,152,233 + IAI47_10180 0.51 -0.8 2,152,233 + IAI47_10180 0.51 -0.0 2,152,234 - IAI47_10180 0.51 -2.3 2,152,234 - IAI47_10180 0.51 +2.0 2,152,234 - IAI47_10180 0.51 +0.4 2,152,234 - IAI47_10180 0.51 +0.6 2,152,234 - IAI47_10180 0.51 +2.2 2,152,234 - IAI47_10180 0.51 +0.7 2,152,234 - IAI47_10180 0.51 +1.5 2,152,234 - IAI47_10180 0.51 -0.6 2,152,234 - IAI47_10180 0.51 -0.0 2,152,234 - IAI47_10180 0.51 +0.6 2,152,234 - IAI47_10180 0.51 -0.2 2,152,237 + IAI47_10180 0.51 -1.0 2,152,237 + IAI47_10180 0.51 -0.6 2,152,238 - IAI47_10180 0.51 -0.4 2,152,238 - IAI47_10180 0.51 +0.1 2,152,252 + IAI47_10180 0.52 -2.2 2,152,252 + IAI47_10180 0.52 -0.4 2,152,252 + IAI47_10180 0.52 +0.6 2,152,252 + IAI47_10180 0.52 -0.2 2,152,333 - IAI47_10180 0.58 +0.4 2,152,381 + IAI47_10180 0.62 +1.4 2,152,381 + IAI47_10180 0.62 -1.1 2,152,426 + IAI47_10180 0.65 -0.7 2,152,426 + IAI47_10180 0.65 +0.9 2,152,426 + IAI47_10180 0.65 -0.5 2,152,426 + IAI47_10180 0.65 +1.0 2,152,427 - IAI47_10180 0.65 +0.5 2,152,517 + IAI47_10180 0.72 -1.2 2,152,517 + IAI47_10180 0.72 -0.6 2,152,518 - IAI47_10180 0.72 +0.0 2,152,545 + IAI47_10180 0.74 +1.4 2,152,545 + IAI47_10180 0.74 +0.5 2,152,545 + +0.1 2,152,545 + IAI47_10180 0.74 +0.4 2,152,545 + IAI47_10180 0.74 +0.8 2,152,545 + IAI47_10180 0.74 +0.1 2,152,546 - IAI47_10180 0.74 +0.6 2,152,546 - IAI47_10180 0.74 +0.8 2,152,546 - IAI47_10180 0.74 +2.0 2,152,546 - IAI47_10180 0.74 +0.9 2,152,546 - IAI47_10180 0.74 +0.6 2,152,546 - IAI47_10180 0.74 +0.7 2,152,546 - IAI47_10180 0.74 -0.2 2,152,549 + IAI47_10180 0.74 +2.3 2,152,549 + IAI47_10180 0.74 +0.0 2,152,549 + IAI47_10180 0.74 +1.0 2,152,549 + IAI47_10180 0.74 -0.3 2,152,549 + IAI47_10180 0.74 +1.3 2,152,549 + IAI47_10180 0.74 +1.2 2,152,550 - IAI47_10180 0.74 -0.0 2,152,550 - IAI47_10180 0.74 +0.2 2,152,550 - IAI47_10180 0.74 +1.1 2,152,550 - IAI47_10180 0.74 -0.3 2,152,576 + IAI47_10180 0.76 +1.5 2,152,577 - IAI47_10180 0.76 -0.1 2,152,649 - IAI47_10180 0.81 -1.4 2,152,656 - IAI47_10180 0.82 +2.0 2,152,656 - IAI47_10180 0.82 +0.4 2,152,656 - IAI47_10180 0.82 +1.4 2,152,656 - IAI47_10180 0.82 -1.4 2,152,666 + IAI47_10180 0.83 -0.7 2,152,667 - IAI47_10180 0.83 -1.3 2,152,667 - IAI47_10180 0.83 -0.7 2,152,668 + IAI47_10180 0.83 +2.2 2,152,678 + IAI47_10180 0.83 -0.4 2,152,703 - IAI47_10180 0.85 -1.7 2,152,729 + IAI47_10180 0.87 -1.7 2,152,729 + IAI47_10180 0.87 +0.5 2,152,729 + IAI47_10180 0.87 +1.5 2,152,729 + IAI47_10180 0.87 -2.9 2,152,729 + IAI47_10180 0.87 -1.2 2,152,730 - IAI47_10180 0.87 +0.0 2,152,730 - IAI47_10180 0.87 +0.4 2,152,730 - IAI47_10180 0.87 +0.1 2,152,730 - IAI47_10180 0.87 -0.7 2,152,730 - IAI47_10180 0.87 +1.1 2,152,732 + IAI47_10180 0.87 -1.1 2,152,732 + IAI47_10180 0.87 -0.7 2,152,732 + IAI47_10180 0.87 +0.2 2,152,732 + IAI47_10180 0.87 -0.2 2,152,751 - IAI47_10180 0.89 -0.0 2,152,751 - IAI47_10180 0.89 +0.6 2,152,754 + IAI47_10180 0.89 -0.4 2,152,775 - -0.6 2,152,837 + -1.7 2,152,897 + +0.2 2,152,982 + IAI47_10185 0.27 -1.1 2,152,988 + IAI47_10185 0.29 -0.6 2,152,989 - IAI47_10185 0.30 -0.0 2,153,000 + IAI47_10185 0.33 +0.6 2,153,001 - IAI47_10185 0.33 +1.0 2,153,001 - IAI47_10185 0.33 -0.2 2,153,029 - IAI47_10185 0.42 -0.6 2,153,029 - IAI47_10185 0.42 +0.5 2,153,029 - IAI47_10185 0.42 -1.0 2,153,063 + IAI47_10185 0.53 -1.3 2,153,063 + IAI47_10185 0.53 +0.1 2,153,064 - IAI47_10185 0.53 +0.6 2,153,073 - IAI47_10185 0.56 -2.2 2,153,073 - IAI47_10185 0.56 -0.4 2,153,133 + IAI47_10185 0.75 -1.0 2,153,133 + IAI47_10185 0.75 +1.2 2,153,137 - IAI47_10185 0.77 -0.6 2,153,196 + -0.0 2,153,196 + +0.5 2,153,226 + -0.2 2,153,226 + -1.0 2,153,226 + -0.4 2,153,227 - -0.6 2,153,227 - -2.0 2,153,227 - +0.5 2,153,227 - -0.7 2,153,228 + -0.6 2,153,228 + +0.4 2,153,229 - -0.2 2,153,251 + -0.0 2,153,293 - -0.5 2,153,293 - -1.2 2,153,293 - +1.2 2,153,315 + -0.4 2,153,331 - IAI47_10190 0.11 -0.3 2,153,342 + IAI47_10190 0.12 -0.4 2,153,363 + IAI47_10190 0.15 +0.8 2,153,364 - IAI47_10190 0.15 +0.0 2,153,450 + IAI47_10190 0.23 +1.6 2,153,457 - IAI47_10190 0.24 -0.7 2,153,457 - IAI47_10190 0.24 +1.2 2,153,457 - IAI47_10190 0.24 -0.7 2,153,531 - IAI47_10190 0.32 -0.7 2,153,531 - IAI47_10190 0.32 +0.0 2,153,542 + IAI47_10190 0.33 +1.7 2,153,542 + IAI47_10190 0.33 -1.2 2,153,543 - IAI47_10190 0.33 -2.3 2,153,664 - IAI47_10190 0.45 -0.4 2,153,682 + IAI47_10190 0.47 +0.5 2,153,706 + IAI47_10190 0.50 +2.4 2,153,706 + IAI47_10190 0.50 +0.5 2,153,750 + IAI47_10190 0.54 +0.0 2,153,751 - IAI47_10190 0.54 -0.0 2,153,773 - IAI47_10190 0.56 +2.5 2,153,810 - IAI47_10190 0.60 -0.0 2,153,844 - IAI47_10190 0.64 +0.6 2,153,923 - IAI47_10190 0.72 +0.5 2,153,923 - IAI47_10190 0.72 +0.4 2,153,923 - IAI47_10190 0.72 +2.1 2,153,925 + IAI47_10190 0.72 +0.6 2,153,925 + IAI47_10190 0.72 -2.0 2,153,925 + IAI47_10190 0.72 -0.1 2,153,925 + IAI47_10190 0.72 -0.3 2,153,926 - IAI47_10190 0.72 -1.6 2,153,926 - IAI47_10190 0.72 +1.1 2,153,926 - IAI47_10190 0.72 -0.4 2,153,933 + IAI47_10190 0.73 +2.0 2,153,933 + IAI47_10190 0.73 -0.3 2,153,933 + IAI47_10190 0.73 +0.6 2,153,934 - IAI47_10190 0.73 -1.8 2,153,934 - IAI47_10190 0.73 -0.0 2,153,934 - IAI47_10190 0.73 +0.4 2,153,961 + IAI47_10190 0.76 -0.6 2,153,961 + IAI47_10190 0.76 -1.3 2,153,962 - IAI47_10190 0.76 +1.4 2,153,969 - IAI47_10190 0.76 +2.0 2,153,969 - IAI47_10190 0.76 -0.3 2,153,969 - IAI47_10190 0.76 -1.6 2,154,060 - IAI47_10190 0.86 +1.9 2,154,071 + IAI47_10190 0.87 -0.7 2,154,071 + IAI47_10190 0.87 -0.4 2,154,071 + IAI47_10190 0.87 +0.6 2,154,072 - IAI47_10190 0.87 -0.0 2,154,072 - IAI47_10190 0.87 +0.3 2,154,072 - IAI47_10190 0.87 -1.1 2,154,105 + -1.9 2,154,106 - -0.2 2,154,135 + -2.2 2,154,135 + -0.3 2,154,153 - -0.4 2,154,153 - +1.3 2,154,157 - -0.2
Or see this region's nucleotide sequence