Experiment: Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=12hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_16150 and IAI47_16155 are separated by 306 nucleotides IAI47_16155 and IAI47_16160 are separated by 36 nucleotides IAI47_16160 and IAI47_16165 are separated by 31 nucleotides
IAI47_16150: IAI47_16150 - bifunctional riboflavin kinase/FAD synthetase, at 3,419,810 to 3,420,739
_16150
IAI47_16155: IAI47_16155 - 30S ribosomal protein S20, at 3,421,046 to 3,421,309
_16155
IAI47_16160: IAI47_16160 - transcriptional activator NhaR, at 3,421,346 to 3,422,263
_16160
IAI47_16165: IAI47_16165 - Na+/H+ antiporter NhaA, at 3,422,295 to 3,423,473
_16165
Position (kb)
3421
3422
3423 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3420.772 kb on - strand at 3420.772 kb on - strand at 3420.772 kb on - strand at 3420.772 kb on - strand at 3420.774 kb on - strand at 3420.774 kb on - strand at 3420.774 kb on - strand at 3420.796 kb on - strand at 3420.823 kb on - strand at 3420.856 kb on + strand at 3420.857 kb on - strand at 3420.857 kb on - strand at 3420.857 kb on - strand at 3420.871 kb on + strand at 3420.876 kb on - strand at 3420.880 kb on + strand at 3420.880 kb on + strand at 3420.921 kb on + strand at 3420.955 kb on - strand at 3421.369 kb on + strand at 3421.370 kb on - strand at 3421.456 kb on + strand, within IAI47_16160 at 3421.456 kb on + strand, within IAI47_16160 at 3421.456 kb on + strand, within IAI47_16160 at 3421.456 kb on + strand, within IAI47_16160 at 3421.456 kb on + strand, within IAI47_16160 at 3421.456 kb on + strand, within IAI47_16160 at 3421.456 kb on + strand, within IAI47_16160 at 3421.456 kb on + strand, within IAI47_16160 at 3421.456 kb on + strand, within IAI47_16160 at 3421.456 kb on + strand, within IAI47_16160 at 3421.456 kb on + strand, within IAI47_16160 at 3421.456 kb on + strand, within IAI47_16160 at 3421.457 kb on - strand, within IAI47_16160 at 3421.457 kb on - strand, within IAI47_16160 at 3421.457 kb on - strand, within IAI47_16160 at 3421.457 kb on - strand, within IAI47_16160 at 3421.457 kb on - strand, within IAI47_16160 at 3421.457 kb on - strand, within IAI47_16160 at 3421.457 kb on - strand, within IAI47_16160 at 3421.457 kb on - strand, within IAI47_16160 at 3421.461 kb on + strand, within IAI47_16160 at 3421.461 kb on + strand, within IAI47_16160 at 3421.461 kb on + strand, within IAI47_16160 at 3421.461 kb on + strand, within IAI47_16160 at 3421.461 kb on + strand, within IAI47_16160 at 3421.462 kb on - strand, within IAI47_16160 at 3421.462 kb on - strand, within IAI47_16160 at 3421.462 kb on - strand, within IAI47_16160 at 3421.462 kb on - strand, within IAI47_16160 at 3421.477 kb on + strand, within IAI47_16160 at 3421.478 kb on - strand, within IAI47_16160 at 3421.478 kb on - strand, within IAI47_16160 at 3421.550 kb on + strand, within IAI47_16160 at 3421.550 kb on + strand, within IAI47_16160 at 3421.550 kb on + strand, within IAI47_16160 at 3421.550 kb on + strand, within IAI47_16160 at 3421.550 kb on + strand, within IAI47_16160 at 3421.551 kb on - strand, within IAI47_16160 at 3421.561 kb on + strand, within IAI47_16160 at 3421.562 kb on - strand, within IAI47_16160 at 3421.562 kb on - strand, within IAI47_16160 at 3421.562 kb on - strand, within IAI47_16160 at 3421.636 kb on + strand, within IAI47_16160 at 3421.637 kb on - strand, within IAI47_16160 at 3421.825 kb on + strand, within IAI47_16160 at 3421.825 kb on + strand, within IAI47_16160 at 3421.825 kb on + strand, within IAI47_16160 at 3421.825 kb on + strand, within IAI47_16160 at 3421.825 kb on + strand, within IAI47_16160 at 3421.825 kb on + strand, within IAI47_16160 at 3421.825 kb on + strand, within IAI47_16160 at 3421.825 kb on + strand, within IAI47_16160 at 3421.825 kb on + strand, within IAI47_16160 at 3421.826 kb on - strand, within IAI47_16160 at 3421.826 kb on - strand, within IAI47_16160 at 3421.826 kb on - strand, within IAI47_16160 at 3421.826 kb on - strand, within IAI47_16160 at 3421.826 kb on - strand, within IAI47_16160 at 3421.831 kb on + strand, within IAI47_16160 at 3421.831 kb on + strand, within IAI47_16160 at 3421.832 kb on - strand, within IAI47_16160 at 3421.832 kb on - strand, within IAI47_16160 at 3421.840 kb on + strand, within IAI47_16160 at 3421.840 kb on + strand, within IAI47_16160 at 3421.840 kb on + strand, within IAI47_16160 at 3421.840 kb on + strand, within IAI47_16160 at 3421.841 kb on - strand, within IAI47_16160 at 3421.841 kb on - strand, within IAI47_16160 at 3421.841 kb on - strand, within IAI47_16160 at 3421.841 kb on - strand, within IAI47_16160 at 3421.936 kb on + strand, within IAI47_16160 at 3421.936 kb on + strand, within IAI47_16160 at 3421.936 kb on + strand, within IAI47_16160 at 3421.936 kb on + strand, within IAI47_16160 at 3421.937 kb on - strand, within IAI47_16160 at 3421.951 kb on + strand, within IAI47_16160 at 3421.951 kb on + strand, within IAI47_16160 at 3422.004 kb on + strand, within IAI47_16160 at 3422.005 kb on - strand, within IAI47_16160 at 3422.005 kb on - strand, within IAI47_16160 at 3422.038 kb on + strand, within IAI47_16160 at 3422.038 kb on + strand, within IAI47_16160 at 3422.038 kb on + strand, within IAI47_16160 at 3422.039 kb on - strand, within IAI47_16160 at 3422.039 kb on - strand, within IAI47_16160 at 3422.039 kb on - strand, within IAI47_16160 at 3422.039 kb on - strand, within IAI47_16160 at 3422.039 kb on - strand, within IAI47_16160 at 3422.039 kb on - strand, within IAI47_16160 at 3422.039 kb on - strand, within IAI47_16160 at 3422.039 kb on - strand, within IAI47_16160 at 3422.146 kb on + strand, within IAI47_16160 at 3422.146 kb on + strand, within IAI47_16160 at 3422.146 kb on + strand, within IAI47_16160 at 3422.146 kb on + strand, within IAI47_16160 at 3422.146 kb on + strand, within IAI47_16160 at 3422.146 kb on + strand, within IAI47_16160 at 3422.147 kb on - strand, within IAI47_16160 at 3422.147 kb on - strand, within IAI47_16160 at 3422.183 kb on + strand at 3422.183 kb on + strand at 3422.183 kb on + strand at 3422.183 kb on + strand at 3422.184 kb on - strand at 3422.184 kb on - strand at 3422.232 kb on + strand at 3422.232 kb on + strand at 3422.232 kb on + strand at 3422.232 kb on + strand at 3422.233 kb on - strand at 3422.235 kb on + strand at 3422.235 kb on + strand at 3422.235 kb on + strand at 3422.322 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=12hrs; Outgrowth=LBkan50 remove 3,420,772 - +1.0 3,420,772 - -0.6 3,420,772 - +0.5 3,420,772 - +0.9 3,420,774 - +0.3 3,420,774 - -0.8 3,420,774 - -0.1 3,420,796 - +0.4 3,420,823 - -0.2 3,420,856 + +0.8 3,420,857 - -0.8 3,420,857 - -0.6 3,420,857 - +1.1 3,420,871 + +0.3 3,420,876 - -0.5 3,420,880 + -0.0 3,420,880 + -0.8 3,420,921 + -0.1 3,420,955 - -0.0 3,421,369 + -1.2 3,421,370 - -0.6 3,421,456 + IAI47_16160 0.12 -1.2 3,421,456 + IAI47_16160 0.12 -0.2 3,421,456 + IAI47_16160 0.12 -0.2 3,421,456 + IAI47_16160 0.12 +0.2 3,421,456 + IAI47_16160 0.12 -3.9 3,421,456 + IAI47_16160 0.12 -0.5 3,421,456 + IAI47_16160 0.12 +0.5 3,421,456 + IAI47_16160 0.12 -1.2 3,421,456 + IAI47_16160 0.12 -0.3 3,421,456 + IAI47_16160 0.12 -1.3 3,421,456 + IAI47_16160 0.12 -3.5 3,421,456 + IAI47_16160 0.12 +0.7 3,421,457 - IAI47_16160 0.12 -1.1 3,421,457 - IAI47_16160 0.12 -0.5 3,421,457 - IAI47_16160 0.12 +0.4 3,421,457 - IAI47_16160 0.12 -0.1 3,421,457 - IAI47_16160 0.12 -1.8 3,421,457 - IAI47_16160 0.12 -0.4 3,421,457 - IAI47_16160 0.12 -1.5 3,421,457 - IAI47_16160 0.12 -0.7 3,421,461 + IAI47_16160 0.13 -2.9 3,421,461 + IAI47_16160 0.13 +0.1 3,421,461 + IAI47_16160 0.13 -1.0 3,421,461 + IAI47_16160 0.13 -1.7 3,421,461 + IAI47_16160 0.13 -0.9 3,421,462 - IAI47_16160 0.13 +1.4 3,421,462 - IAI47_16160 0.13 -0.9 3,421,462 - IAI47_16160 0.13 +0.2 3,421,462 - IAI47_16160 0.13 -0.7 3,421,477 + IAI47_16160 0.14 -0.6 3,421,478 - IAI47_16160 0.14 +0.0 3,421,478 - IAI47_16160 0.14 -2.0 3,421,550 + IAI47_16160 0.22 -1.0 3,421,550 + IAI47_16160 0.22 +0.1 3,421,550 + IAI47_16160 0.22 -0.5 3,421,550 + IAI47_16160 0.22 -0.4 3,421,550 + IAI47_16160 0.22 +0.4 3,421,551 - IAI47_16160 0.22 -0.5 3,421,561 + IAI47_16160 0.23 +0.2 3,421,562 - IAI47_16160 0.24 -1.0 3,421,562 - IAI47_16160 0.24 +0.3 3,421,562 - IAI47_16160 0.24 -1.3 3,421,636 + IAI47_16160 0.32 -0.7 3,421,637 - IAI47_16160 0.32 +0.4 3,421,825 + IAI47_16160 0.52 -0.5 3,421,825 + IAI47_16160 0.52 -1.5 3,421,825 + IAI47_16160 0.52 -0.9 3,421,825 + IAI47_16160 0.52 -0.5 3,421,825 + IAI47_16160 0.52 -1.3 3,421,825 + IAI47_16160 0.52 -0.2 3,421,825 + IAI47_16160 0.52 -1.1 3,421,825 + IAI47_16160 0.52 -1.5 3,421,825 + IAI47_16160 0.52 +0.1 3,421,826 - IAI47_16160 0.52 +1.1 3,421,826 - IAI47_16160 0.52 -0.9 3,421,826 - IAI47_16160 0.52 -0.5 3,421,826 - IAI47_16160 0.52 +0.6 3,421,826 - IAI47_16160 0.52 +0.2 3,421,831 + IAI47_16160 0.53 -2.0 3,421,831 + IAI47_16160 0.53 -0.6 3,421,832 - IAI47_16160 0.53 -1.2 3,421,832 - IAI47_16160 0.53 -0.3 3,421,840 + IAI47_16160 0.54 -1.0 3,421,840 + IAI47_16160 0.54 -0.1 3,421,840 + IAI47_16160 0.54 +1.6 3,421,840 + IAI47_16160 0.54 +0.0 3,421,841 - IAI47_16160 0.54 +0.9 3,421,841 - IAI47_16160 0.54 -0.4 3,421,841 - IAI47_16160 0.54 -0.9 3,421,841 - IAI47_16160 0.54 -0.2 3,421,936 + IAI47_16160 0.64 +0.5 3,421,936 + IAI47_16160 0.64 -0.3 3,421,936 + IAI47_16160 0.64 -0.3 3,421,936 + IAI47_16160 0.64 +0.2 3,421,937 - IAI47_16160 0.64 +0.3 3,421,951 + IAI47_16160 0.66 -0.9 3,421,951 + IAI47_16160 0.66 -0.6 3,422,004 + IAI47_16160 0.72 +0.2 3,422,005 - IAI47_16160 0.72 -2.7 3,422,005 - IAI47_16160 0.72 +0.4 3,422,038 + IAI47_16160 0.75 -0.7 3,422,038 + IAI47_16160 0.75 -0.4 3,422,038 + IAI47_16160 0.75 -0.4 3,422,039 - IAI47_16160 0.75 -1.2 3,422,039 - IAI47_16160 0.75 +0.1 3,422,039 - IAI47_16160 0.75 -0.2 3,422,039 - IAI47_16160 0.75 -0.3 3,422,039 - IAI47_16160 0.75 +0.2 3,422,039 - IAI47_16160 0.75 -0.1 3,422,039 - IAI47_16160 0.75 +0.4 3,422,039 - IAI47_16160 0.75 -0.2 3,422,146 + IAI47_16160 0.87 +0.0 3,422,146 + IAI47_16160 0.87 -0.1 3,422,146 + IAI47_16160 0.87 +0.7 3,422,146 + IAI47_16160 0.87 +0.3 3,422,146 + IAI47_16160 0.87 -0.1 3,422,146 + IAI47_16160 0.87 -0.2 3,422,147 - IAI47_16160 0.87 -0.6 3,422,147 - IAI47_16160 0.87 +0.6 3,422,183 + -0.2 3,422,183 + -1.3 3,422,183 + +1.9 3,422,183 + -0.2 3,422,184 - -0.2 3,422,184 - +0.5 3,422,232 + +0.9 3,422,232 + +0.2 3,422,232 + -0.2 3,422,232 + +0.3 3,422,233 - -0.6 3,422,235 + -0.2 3,422,235 + -1.4 3,422,235 + -1.0 3,422,322 - +2.0
Or see this region's nucleotide sequence