Experiment: m.b. Polymyxin B sulfate 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3030 and HP15_3031 are separated by 100 nucleotides HP15_3031 and HP15_3032 are separated by 12 nucleotides HP15_3032 and HP15_3033 are separated by 3 nucleotides
HP15_3030: HP15_3030 - histidine/lysine/arginine/ornithine transporter subunit, at 3,174,688 to 3,175,458
_3030
HP15_3031: HP15_3031 - L-arginine ABC transporter, periplasmic substrate-binding component ArtJ/HisJ/ArtI/AotJ/ArgT (from data) , at 3,175,559 to 3,176,311
_3031
HP15_3032: HP15_3032 - amino acid ABC transporter, inner membrane subunit, at 3,176,324 to 3,177,067
_3032
HP15_3033: HP15_3033 - amino acid ABC transporter, inner membrane subunit, at 3,177,071 to 3,177,793
_3033
Position (kb)
3175
3176
3177 Strain fitness (log2 ratio)
-1
0
1
2 at 3174.613 kb on + strand at 3174.703 kb on + strand at 3174.711 kb on + strand at 3174.731 kb on + strand at 3174.739 kb on - strand at 3174.740 kb on + strand at 3174.741 kb on + strand at 3174.750 kb on + strand at 3174.814 kb on - strand, within HP15_3030 at 3174.839 kb on + strand, within HP15_3030 at 3174.839 kb on + strand, within HP15_3030 at 3174.900 kb on + strand, within HP15_3030 at 3174.908 kb on - strand, within HP15_3030 at 3174.926 kb on - strand, within HP15_3030 at 3175.128 kb on + strand, within HP15_3030 at 3175.128 kb on + strand, within HP15_3030 at 3175.128 kb on + strand, within HP15_3030 at 3175.136 kb on - strand, within HP15_3030 at 3175.212 kb on - strand, within HP15_3030 at 3175.258 kb on + strand, within HP15_3030 at 3175.260 kb on + strand, within HP15_3030 at 3175.368 kb on + strand, within HP15_3030 at 3175.427 kb on + strand at 3175.440 kb on - strand at 3175.465 kb on + strand at 3175.478 kb on - strand at 3175.478 kb on - strand at 3175.717 kb on + strand, within HP15_3031 at 3175.784 kb on + strand, within HP15_3031 at 3175.959 kb on - strand, within HP15_3031 at 3175.993 kb on - strand, within HP15_3031 at 3176.057 kb on + strand, within HP15_3031 at 3176.066 kb on + strand, within HP15_3031 at 3176.161 kb on + strand, within HP15_3031 at 3176.166 kb on + strand, within HP15_3031 at 3176.189 kb on + strand, within HP15_3031 at 3176.213 kb on - strand, within HP15_3031 at 3176.213 kb on - strand, within HP15_3031 at 3176.269 kb on - strand at 3176.454 kb on + strand, within HP15_3032 at 3176.462 kb on - strand, within HP15_3032 at 3176.462 kb on - strand, within HP15_3032 at 3176.518 kb on + strand, within HP15_3032 at 3176.526 kb on - strand, within HP15_3032 at 3176.533 kb on + strand, within HP15_3032 at 3176.544 kb on + strand, within HP15_3032 at 3176.752 kb on + strand, within HP15_3032 at 3176.760 kb on - strand, within HP15_3032 at 3176.770 kb on - strand, within HP15_3032 at 3176.770 kb on - strand, within HP15_3032 at 3176.776 kb on - strand, within HP15_3032 at 3176.839 kb on - strand, within HP15_3032 at 3176.952 kb on - strand, within HP15_3032 at 3177.053 kb on + strand at 3177.053 kb on + strand at 3177.144 kb on - strand, within HP15_3033 at 3177.159 kb on + strand, within HP15_3033 at 3177.239 kb on + strand, within HP15_3033 at 3177.262 kb on + strand, within HP15_3033 at 3177.270 kb on - strand, within HP15_3033 at 3177.304 kb on + strand, within HP15_3033
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0004 mg/ml remove 3,174,613 + +0.2 3,174,703 + +1.0 3,174,711 + +0.5 3,174,731 + +0.5 3,174,739 - -0.3 3,174,740 + +0.8 3,174,741 + +0.4 3,174,750 + +1.0 3,174,814 - HP15_3030 0.16 -0.4 3,174,839 + HP15_3030 0.20 -0.9 3,174,839 + HP15_3030 0.20 +0.6 3,174,900 + HP15_3030 0.27 +1.7 3,174,908 - HP15_3030 0.29 -0.2 3,174,926 - HP15_3030 0.31 +0.4 3,175,128 + HP15_3030 0.57 +0.7 3,175,128 + HP15_3030 0.57 +1.0 3,175,128 + HP15_3030 0.57 +0.7 3,175,136 - HP15_3030 0.58 +0.6 3,175,212 - HP15_3030 0.68 +0.4 3,175,258 + HP15_3030 0.74 +1.9 3,175,260 + HP15_3030 0.74 +0.5 3,175,368 + HP15_3030 0.88 +1.3 3,175,427 + +0.8 3,175,440 - +0.4 3,175,465 + -0.1 3,175,478 - +0.4 3,175,478 - +1.3 3,175,717 + HP15_3031 0.21 +1.5 3,175,784 + HP15_3031 0.30 +1.2 3,175,959 - HP15_3031 0.53 +0.8 3,175,993 - HP15_3031 0.58 +0.6 3,176,057 + HP15_3031 0.66 +1.3 3,176,066 + HP15_3031 0.67 +1.2 3,176,161 + HP15_3031 0.80 -0.5 3,176,166 + HP15_3031 0.81 +1.3 3,176,189 + HP15_3031 0.84 +0.9 3,176,213 - HP15_3031 0.87 +0.8 3,176,213 - HP15_3031 0.87 +1.2 3,176,269 - +0.4 3,176,454 + HP15_3032 0.17 +0.1 3,176,462 - HP15_3032 0.19 +0.2 3,176,462 - HP15_3032 0.19 +0.8 3,176,518 + HP15_3032 0.26 +1.9 3,176,526 - HP15_3032 0.27 +0.5 3,176,533 + HP15_3032 0.28 +0.6 3,176,544 + HP15_3032 0.30 +1.3 3,176,752 + HP15_3032 0.58 +1.0 3,176,760 - HP15_3032 0.59 +0.9 3,176,770 - HP15_3032 0.60 +1.1 3,176,770 - HP15_3032 0.60 +0.6 3,176,776 - HP15_3032 0.61 -0.1 3,176,839 - HP15_3032 0.69 +0.9 3,176,952 - HP15_3032 0.84 +1.2 3,177,053 + +0.1 3,177,053 + +1.0 3,177,144 - HP15_3033 0.10 +0.7 3,177,159 + HP15_3033 0.12 +0.4 3,177,239 + HP15_3033 0.23 +0.1 3,177,262 + HP15_3033 0.26 +1.0 3,177,270 - HP15_3033 0.28 +0.7 3,177,304 + HP15_3033 0.32 +0.9
Or see this region's nucleotide sequence