Experiment: m.b. Polymyxin B sulfate 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_217 and HP15_218 are separated by 122 nucleotides HP15_218 and HP15_219 are separated by 20 nucleotides HP15_219 and HP15_220 are separated by 150 nucleotides HP15_220 and HP15_222 overlap by 18 nucleotides HP15_222 and HP15_221 are separated by 42 nucleotides HP15_221 and HP15_223 are separated by 95 nucleotides
HP15_217: HP15_217 - glutamate--cysteine ligase, at 205,710 to 207,263
_217
HP15_218: HP15_218 - conserved hypothetical protein, at 207,386 to 207,601
_218
HP15_219: HP15_219 - flagellar basal body-associated protein FliL, at 207,622 to 208,071
_219
HP15_220: HP15_220 - disulfide bond formation protein B, at 208,222 to 208,629
_220
HP15_222: HP15_222 - hypothetical protein, at 208,612 to 208,728
_222
HP15_221: HP15_221 - anti-RNA polymerase sigma 70 factor, at 208,771 to 209,250
_221
HP15_223: HP15_223 - peptidylprolyl isomerase, FKBP-type, at 209,346 to 210,062
_223
Position (kb) 208
209 Strain fitness (log2 ratio) -2
-1
0
1 at 207.277 kb on - strand at 207.427 kb on + strand, within HP15_218 at 207.455 kb on - strand, within HP15_218 at 207.560 kb on - strand, within HP15_218 at 208.110 kb on + strand at 208.282 kb on - strand, within HP15_220 at 208.308 kb on + strand, within HP15_220 at 208.444 kb on - strand, within HP15_220 at 208.486 kb on - strand, within HP15_220 at 208.505 kb on - strand, within HP15_220 at 208.564 kb on + strand, within HP15_220 at 208.572 kb on - strand, within HP15_220 at 208.811 kb on + strand at 208.835 kb on + strand, within HP15_221 at 208.868 kb on + strand, within HP15_221 at 209.032 kb on - strand, within HP15_221 at 209.368 kb on + strand at 209.397 kb on + strand at 209.402 kb on + strand at 209.405 kb on - strand at 209.543 kb on - strand, within HP15_223
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0004 mg/ml remove 207,277 - +1.0 207,427 + HP15_218 0.19 +0.4 207,455 - HP15_218 0.32 +0.7 207,560 - HP15_218 0.81 -0.8 208,110 + -0.5 208,282 - HP15_220 0.15 -0.1 208,308 + HP15_220 0.21 +0.9 208,444 - HP15_220 0.54 -2.1 208,486 - HP15_220 0.65 -1.4 208,505 - HP15_220 0.69 -1.3 208,564 + HP15_220 0.84 -2.7 208,572 - HP15_220 0.86 -0.7 208,811 + -0.1 208,835 + HP15_221 0.13 +0.0 208,868 + HP15_221 0.20 +0.0 209,032 - HP15_221 0.54 +0.9 209,368 + -1.1 209,397 + -0.8 209,402 + -0.9 209,405 - -0.1 209,543 - HP15_223 0.27 -0.8
Or see this region's nucleotide sequence