Experiment: m.b. Polymyxin B sulfate 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_209 and HP15_210 are separated by 143 nucleotides HP15_210 and HP15_211 are separated by 11 nucleotides HP15_211 and HP15_212 overlap by 25 nucleotides HP15_212 and HP15_213 are separated by 146 nucleotides
HP15_209: HP15_209 - phosphomannomutase, at 195,696 to 197,054
_209
HP15_210: HP15_210 - phospholipase A(1), at 197,198 to 198,325
_210
HP15_211: HP15_211 - conserved hypothetical protein, membrane, at 198,337 to 198,675
_211
HP15_212: HP15_212 - hypothetical protein, at 198,651 to 198,788
_212
HP15_213: HP15_213 - cytochrome c5, at 198,935 to 199,291
_213
Position (kb) 197
198
199 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 196.482 kb on + strand, within HP15_209 at 196.516 kb on - strand, within HP15_209 at 196.643 kb on - strand, within HP15_209 at 196.743 kb on + strand, within HP15_209 at 196.751 kb on - strand, within HP15_209 at 196.826 kb on + strand, within HP15_209 at 197.002 kb on - strand at 197.121 kb on + strand at 197.155 kb on + strand at 197.172 kb on + strand at 197.215 kb on + strand at 197.215 kb on + strand at 197.223 kb on - strand at 197.234 kb on - strand at 197.297 kb on + strand at 197.307 kb on - strand at 197.411 kb on + strand, within HP15_210 at 197.445 kb on - strand, within HP15_210 at 197.594 kb on - strand, within HP15_210 at 197.594 kb on - strand, within HP15_210 at 197.710 kb on + strand, within HP15_210 at 197.717 kb on + strand, within HP15_210 at 197.725 kb on - strand, within HP15_210 at 197.743 kb on + strand, within HP15_210 at 197.751 kb on - strand, within HP15_210 at 197.800 kb on + strand, within HP15_210 at 197.925 kb on - strand, within HP15_210 at 197.925 kb on - strand, within HP15_210 at 198.011 kb on - strand, within HP15_210 at 198.029 kb on + strand, within HP15_210 at 198.107 kb on - strand, within HP15_210 at 198.123 kb on + strand, within HP15_210 at 198.137 kb on - strand, within HP15_210 at 198.203 kb on + strand, within HP15_210 at 198.222 kb on - strand at 198.359 kb on - strand at 198.403 kb on + strand, within HP15_211 at 198.609 kb on - strand, within HP15_211 at 198.748 kb on - strand, within HP15_212 at 198.813 kb on + strand at 198.821 kb on - strand at 198.885 kb on - strand at 198.909 kb on + strand at 199.022 kb on + strand, within HP15_213 at 199.041 kb on + strand, within HP15_213 at 199.148 kb on + strand, within HP15_213 at 199.258 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0004 mg/ml remove 196,482 + HP15_209 0.58 +0.4 196,516 - HP15_209 0.60 -1.0 196,643 - HP15_209 0.70 +0.6 196,743 + HP15_209 0.77 +0.2 196,751 - HP15_209 0.78 -1.1 196,826 + HP15_209 0.83 -0.7 197,002 - -0.3 197,121 + +0.3 197,155 + -0.5 197,172 + +0.1 197,215 + +3.0 197,215 + +3.8 197,223 - +3.6 197,234 - +3.0 197,297 + +3.3 197,307 - +2.9 197,411 + HP15_210 0.19 +3.0 197,445 - HP15_210 0.22 +3.1 197,594 - HP15_210 0.35 +3.3 197,594 - HP15_210 0.35 +5.1 197,710 + HP15_210 0.45 +2.9 197,717 + HP15_210 0.46 +3.5 197,725 - HP15_210 0.47 +3.2 197,743 + HP15_210 0.48 +2.7 197,751 - HP15_210 0.49 +3.3 197,800 + HP15_210 0.53 +3.2 197,925 - HP15_210 0.64 +3.0 197,925 - HP15_210 0.64 +3.2 198,011 - HP15_210 0.72 +3.0 198,029 + HP15_210 0.74 +3.2 198,107 - HP15_210 0.81 +3.2 198,123 + HP15_210 0.82 +2.8 198,137 - HP15_210 0.83 +3.2 198,203 + HP15_210 0.89 +3.2 198,222 - +3.4 198,359 - +2.2 198,403 + HP15_211 0.19 +3.2 198,609 - HP15_211 0.80 +1.8 198,748 - HP15_212 0.70 +1.1 198,813 + +0.4 198,821 - -2.4 198,885 - -4.0 198,909 + -2.0 199,022 + HP15_213 0.24 -3.2 199,041 + HP15_213 0.30 -0.3 199,148 + HP15_213 0.60 -3.1 199,258 - -2.3
Or see this region's nucleotide sequence