Experiment: m.b. Polymyxin B sulfate 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1320 and HP15_1321 are separated by 210 nucleotides HP15_1321 and HP15_tRNA22 are separated by 126 nucleotides HP15_tRNA22 and HP15_tRNA23 are separated by 64 nucleotides HP15_tRNA23 and HP15_tRNA24 are separated by 25 nucleotides HP15_tRNA24 and HP15_1322 are separated by 453 nucleotides
HP15_1320: HP15_1320 - aspartate kinase: aspartate kinase, monofunctional class, at 1,383,714 to 1,384,952
_1320
HP15_1321: HP15_1321 - carbon storage regulator, at 1,385,163 to 1,385,339
_1321
HP15_tRNA22: HP15_tRNA22 - tRNA-Ser, at 1,385,466 to 1,385,555
_tRNA22
HP15_tRNA23: HP15_tRNA23 - tRNA-Arg, at 1,385,620 to 1,385,696
_tRNA23
HP15_tRNA24: HP15_tRNA24 - tRNA-Arg, at 1,385,722 to 1,385,798
_tRNA24
HP15_1322: HP15_1322 - conserved hypothetical protein, at 1,386,252 to 1,387,145
_1322
Position (kb)
1385
1386 Strain fitness (log2 ratio)
-2
-1
0
1 at 1385.119 kb on + strand at 1385.308 kb on + strand, within HP15_1321 at 1385.850 kb on + strand at 1385.908 kb on + strand at 1385.919 kb on + strand at 1385.948 kb on - strand at 1386.046 kb on + strand at 1386.046 kb on + strand at 1386.046 kb on + strand at 1386.054 kb on - strand at 1386.056 kb on + strand at 1386.064 kb on - strand at 1386.201 kb on + strand at 1386.203 kb on + strand at 1386.267 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0004 mg/ml remove 1,385,119 + +0.7 1,385,308 + HP15_1321 0.82 -2.1 1,385,850 + -0.7 1,385,908 + -0.9 1,385,919 + +0.8 1,385,948 - +0.1 1,386,046 + +0.3 1,386,046 + -0.3 1,386,046 + +1.3 1,386,054 - -0.2 1,386,056 + +0.1 1,386,064 - -0.7 1,386,201 + +1.5 1,386,203 + +0.3 1,386,267 - +0.2
Or see this region's nucleotide sequence