Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04800 and BWI76_RS04805 are separated by 60 nucleotides BWI76_RS04805 and BWI76_RS04810 are separated by 52 nucleotides BWI76_RS04810 and BWI76_RS04815 are separated by 9 nucleotides BWI76_RS04815 and BWI76_RS04820 overlap by 1 nucleotides
BWI76_RS04800: BWI76_RS04800 - preprotein translocase subunit SecA, at 1,002,596 to 1,005,301
_RS04800
BWI76_RS04805: BWI76_RS04805 - 8-oxo-dGTP diphosphatase, at 1,005,362 to 1,005,769
_RS04805
BWI76_RS04810: BWI76_RS04810 - DNA gyrase inhibitor YacG, at 1,005,822 to 1,006,016
_RS04810
BWI76_RS04815: BWI76_RS04815 - cell division protein ZapD, at 1,006,026 to 1,006,769
_RS04815
BWI76_RS04820: BWI76_RS04820 - dephospho-CoA kinase, at 1,006,769 to 1,007,389
_RS04820
Position (kb) 1005
1006 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1005.103 kb on + strand at 1005.124 kb on - strand at 1005.295 kb on + strand at 1005.545 kb on - strand, within BWI76_RS04805 at 1005.682 kb on - strand, within BWI76_RS04805 at 1006.038 kb on - strand at 1006.523 kb on + strand, within BWI76_RS04815 at 1006.583 kb on + strand, within BWI76_RS04815 at 1006.613 kb on - strand, within BWI76_RS04815 at 1006.623 kb on - strand, within BWI76_RS04815
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,005,103 + -1.9 1,005,124 - -0.9 1,005,295 + -1.2 1,005,545 - BWI76_RS04805 0.45 -0.1 1,005,682 - BWI76_RS04805 0.78 +1.9 1,006,038 - -0.5 1,006,523 + BWI76_RS04815 0.67 -0.3 1,006,583 + BWI76_RS04815 0.75 -0.1 1,006,613 - BWI76_RS04815 0.79 -0.2 1,006,623 - BWI76_RS04815 0.80 -2.6
Or see this region's nucleotide sequence