Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Xcc-8004.2320.1 and Xcc-8004.2321.1 are separated by 5 nucleotides Xcc-8004.2321.1 and Xcc-8004.2322.1 overlap by 4 nucleotides
Xcc-8004.2320.1: Xcc-8004.2320.1 - Acetylglutamate kinase (EC 2.7.2.8), at 2,267,246 to 2,268,589
Xcc-8004.2320.1
Xcc-8004.2321.1: Xcc-8004.2321.1 - N-acetylglutamate synthase (EC 2.3.1.1), at 2,268,595 to 2,269,206
Xcc-8004.2321.1
Xcc-8004.2322.1: Xcc-8004.2322.1 - N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38), at 2,269,203 to 2,270,153
Xcc-8004.2322.1
Position (kb)
2268
2269
2270 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2267.699 kb on + strand, within Xcc-8004.2320.1 at 2267.699 kb on + strand, within Xcc-8004.2320.1 at 2267.699 kb on + strand, within Xcc-8004.2320.1 at 2267.699 kb on + strand, within Xcc-8004.2320.1 at 2267.700 kb on - strand, within Xcc-8004.2320.1 at 2267.700 kb on - strand, within Xcc-8004.2320.1 at 2267.867 kb on + strand, within Xcc-8004.2320.1 at 2267.867 kb on + strand, within Xcc-8004.2320.1 at 2267.951 kb on + strand, within Xcc-8004.2320.1 at 2268.225 kb on - strand, within Xcc-8004.2320.1 at 2268.254 kb on + strand, within Xcc-8004.2320.1 at 2268.254 kb on + strand, within Xcc-8004.2320.1 at 2268.254 kb on + strand, within Xcc-8004.2320.1 at 2268.254 kb on + strand, within Xcc-8004.2320.1 at 2268.254 kb on + strand, within Xcc-8004.2320.1 at 2268.255 kb on - strand, within Xcc-8004.2320.1 at 2268.255 kb on - strand, within Xcc-8004.2320.1 at 2268.269 kb on + strand, within Xcc-8004.2320.1 at 2268.326 kb on + strand, within Xcc-8004.2320.1 at 2268.327 kb on - strand, within Xcc-8004.2320.1 at 2268.327 kb on - strand, within Xcc-8004.2320.1 at 2268.458 kb on + strand at 2268.458 kb on + strand at 2268.459 kb on - strand at 2268.461 kb on + strand at 2268.736 kb on + strand, within Xcc-8004.2321.1 at 2268.772 kb on + strand, within Xcc-8004.2321.1 at 2268.772 kb on + strand, within Xcc-8004.2321.1 at 2268.773 kb on - strand, within Xcc-8004.2321.1 at 2268.866 kb on - strand, within Xcc-8004.2321.1 at 2268.866 kb on - strand, within Xcc-8004.2321.1 at 2268.868 kb on + strand, within Xcc-8004.2321.1 at 2268.868 kb on + strand, within Xcc-8004.2321.1 at 2268.868 kb on + strand, within Xcc-8004.2321.1 at 2268.869 kb on - strand, within Xcc-8004.2321.1 at 2269.359 kb on + strand, within Xcc-8004.2322.1 at 2269.360 kb on - strand, within Xcc-8004.2322.1 at 2269.360 kb on - strand, within Xcc-8004.2322.1 at 2269.542 kb on + strand, within Xcc-8004.2322.1 at 2269.542 kb on + strand, within Xcc-8004.2322.1 at 2269.542 kb on + strand, within Xcc-8004.2322.1 at 2269.543 kb on - strand, within Xcc-8004.2322.1 at 2269.543 kb on - strand, within Xcc-8004.2322.1 at 2269.545 kb on + strand, within Xcc-8004.2322.1 at 2269.545 kb on + strand, within Xcc-8004.2322.1 at 2269.546 kb on - strand, within Xcc-8004.2322.1 at 2269.546 kb on - strand, within Xcc-8004.2322.1 at 2269.611 kb on + strand, within Xcc-8004.2322.1 at 2269.611 kb on + strand, within Xcc-8004.2322.1 at 2269.761 kb on + strand, within Xcc-8004.2322.1 at 2269.932 kb on + strand, within Xcc-8004.2322.1
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 2,267,699 + Xcc-8004.2320.1 0.34 +1.8 2,267,699 + Xcc-8004.2320.1 0.34 +0.0 2,267,699 + Xcc-8004.2320.1 0.34 -1.5 2,267,699 + Xcc-8004.2320.1 0.34 +0.2 2,267,700 - Xcc-8004.2320.1 0.34 -0.2 2,267,700 - Xcc-8004.2320.1 0.34 -0.4 2,267,867 + Xcc-8004.2320.1 0.46 -1.2 2,267,867 + Xcc-8004.2320.1 0.46 -0.7 2,267,951 + Xcc-8004.2320.1 0.52 +0.8 2,268,225 - Xcc-8004.2320.1 0.73 -0.6 2,268,254 + Xcc-8004.2320.1 0.75 -0.6 2,268,254 + Xcc-8004.2320.1 0.75 -2.1 2,268,254 + Xcc-8004.2320.1 0.75 -0.6 2,268,254 + Xcc-8004.2320.1 0.75 -0.5 2,268,254 + Xcc-8004.2320.1 0.75 +0.5 2,268,255 - Xcc-8004.2320.1 0.75 -1.3 2,268,255 - Xcc-8004.2320.1 0.75 +0.4 2,268,269 + Xcc-8004.2320.1 0.76 +0.3 2,268,326 + Xcc-8004.2320.1 0.80 -0.7 2,268,327 - Xcc-8004.2320.1 0.80 -0.2 2,268,327 - Xcc-8004.2320.1 0.80 -0.3 2,268,458 + -2.9 2,268,458 + -0.2 2,268,459 - -1.6 2,268,461 + +1.6 2,268,736 + Xcc-8004.2321.1 0.23 -0.2 2,268,772 + Xcc-8004.2321.1 0.29 -1.8 2,268,772 + Xcc-8004.2321.1 0.29 -1.7 2,268,773 - Xcc-8004.2321.1 0.29 -0.2 2,268,866 - Xcc-8004.2321.1 0.44 +0.2 2,268,866 - Xcc-8004.2321.1 0.44 -1.4 2,268,868 + Xcc-8004.2321.1 0.45 +0.5 2,268,868 + Xcc-8004.2321.1 0.45 -1.6 2,268,868 + Xcc-8004.2321.1 0.45 -1.9 2,268,869 - Xcc-8004.2321.1 0.45 +0.4 2,269,359 + Xcc-8004.2322.1 0.16 -1.1 2,269,360 - Xcc-8004.2322.1 0.17 +1.3 2,269,360 - Xcc-8004.2322.1 0.17 +4.5 2,269,542 + Xcc-8004.2322.1 0.36 -0.2 2,269,542 + Xcc-8004.2322.1 0.36 +0.5 2,269,542 + Xcc-8004.2322.1 0.36 -0.8 2,269,543 - Xcc-8004.2322.1 0.36 -0.3 2,269,543 - Xcc-8004.2322.1 0.36 +4.0 2,269,545 + Xcc-8004.2322.1 0.36 +0.8 2,269,545 + Xcc-8004.2322.1 0.36 -1.8 2,269,546 - Xcc-8004.2322.1 0.36 -0.6 2,269,546 - Xcc-8004.2322.1 0.36 -1.0 2,269,611 + Xcc-8004.2322.1 0.43 +0.1 2,269,611 + Xcc-8004.2322.1 0.43 -0.5 2,269,761 + Xcc-8004.2322.1 0.59 +0.2 2,269,932 + Xcc-8004.2322.1 0.77 +0.5
Or see this region's nucleotide sequence