Experiment: Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=12hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_21290 and IAI47_21295 are separated by 149 nucleotides IAI47_21295 and IAI47_21300 are separated by 103 nucleotides IAI47_21300 and IAI47_21305 overlap by 4 nucleotides IAI47_21305 and IAI47_21310 are separated by 111 nucleotides
IAI47_21290: IAI47_21290 - alpha/beta hydrolase, at 514,203 to 514,680
_21290
IAI47_21295: IAI47_21295 - DJ-1/PfpI family protein, at 514,830 to 515,549
_21295
IAI47_21300: IAI47_21300 - AzlD domain-containing protein, at 515,653 to 515,976
_21300
IAI47_21305: IAI47_21305 - AzlC family ABC transporter permease, at 515,973 to 516,641
_21305
IAI47_21310: IAI47_21310 - helix-turn-helix domain-containing protein, at 516,753 to 517,301
_21310
Position (kb) 515
516 Strain fitness (log2 ratio) -2
-1
0
1
2 at 514.681 kb on + strand at 514.682 kb on - strand at 514.693 kb on + strand at 514.693 kb on + strand at 514.694 kb on - strand at 514.700 kb on - strand at 514.734 kb on - strand at 514.734 kb on - strand at 514.776 kb on - strand at 514.822 kb on + strand at 514.822 kb on + strand at 514.822 kb on + strand at 514.823 kb on - strand at 514.823 kb on - strand at 514.831 kb on + strand at 514.831 kb on + strand at 514.831 kb on + strand at 514.832 kb on - strand at 514.832 kb on - strand at 514.838 kb on + strand at 514.838 kb on + strand at 514.839 kb on - strand at 514.839 kb on - strand at 514.869 kb on + strand at 514.913 kb on + strand, within IAI47_21295 at 514.913 kb on + strand, within IAI47_21295 at 514.913 kb on + strand, within IAI47_21295 at 514.914 kb on - strand, within IAI47_21295 at 515.002 kb on + strand, within IAI47_21295 at 515.002 kb on + strand, within IAI47_21295 at 515.002 kb on + strand, within IAI47_21295 at 515.003 kb on - strand, within IAI47_21295 at 515.013 kb on + strand, within IAI47_21295 at 515.014 kb on - strand, within IAI47_21295 at 515.014 kb on - strand, within IAI47_21295 at 515.204 kb on + strand, within IAI47_21295 at 515.204 kb on + strand, within IAI47_21295 at 515.205 kb on - strand, within IAI47_21295 at 515.205 kb on - strand, within IAI47_21295 at 515.241 kb on - strand, within IAI47_21295 at 515.252 kb on + strand, within IAI47_21295 at 515.252 kb on + strand, within IAI47_21295 at 515.252 kb on + strand, within IAI47_21295 at 515.252 kb on + strand, within IAI47_21295 at 515.252 kb on + strand, within IAI47_21295 at 515.252 kb on + strand, within IAI47_21295 at 515.252 kb on + strand, within IAI47_21295 at 515.253 kb on - strand, within IAI47_21295 at 515.253 kb on - strand, within IAI47_21295 at 515.253 kb on - strand, within IAI47_21295 at 515.253 kb on - strand, within IAI47_21295 at 515.253 kb on - strand, within IAI47_21295 at 515.253 kb on - strand, within IAI47_21295 at 515.303 kb on + strand, within IAI47_21295 at 515.304 kb on - strand, within IAI47_21295 at 515.369 kb on + strand, within IAI47_21295 at 515.369 kb on + strand, within IAI47_21295 at 515.369 kb on + strand, within IAI47_21295 at 515.370 kb on - strand, within IAI47_21295 at 515.370 kb on - strand, within IAI47_21295 at 515.370 kb on - strand, within IAI47_21295 at 515.370 kb on - strand, within IAI47_21295 at 515.370 kb on - strand, within IAI47_21295 at 515.370 kb on - strand, within IAI47_21295 at 515.370 kb on - strand, within IAI47_21295 at 515.370 kb on - strand, within IAI47_21295 at 515.392 kb on - strand, within IAI47_21295 at 515.520 kb on + strand at 515.521 kb on - strand at 515.521 kb on - strand at 515.521 kb on - strand at 515.549 kb on + strand at 515.549 kb on + strand at 515.549 kb on + strand at 515.549 kb on + strand at 515.590 kb on + strand at 515.604 kb on + strand at 515.604 kb on + strand at 515.604 kb on + strand at 515.605 kb on - strand at 515.605 kb on - strand at 515.628 kb on + strand at 515.628 kb on + strand at 515.629 kb on - strand at 515.660 kb on - strand at 515.674 kb on + strand at 515.674 kb on + strand at 515.674 kb on + strand at 515.740 kb on + strand, within IAI47_21300 at 515.740 kb on + strand, within IAI47_21300 at 515.740 kb on + strand, within IAI47_21300 at 515.741 kb on - strand, within IAI47_21300 at 515.743 kb on + strand, within IAI47_21300 at 515.854 kb on + strand, within IAI47_21300 at 515.854 kb on + strand, within IAI47_21300 at 515.854 kb on + strand, within IAI47_21300 at 515.855 kb on - strand, within IAI47_21300 at 515.921 kb on + strand, within IAI47_21300 at 515.921 kb on + strand, within IAI47_21300 at 515.921 kb on + strand, within IAI47_21300 at 515.921 kb on + strand, within IAI47_21300 at 515.921 kb on + strand, within IAI47_21300 at 515.921 kb on + strand, within IAI47_21300 at 515.921 kb on + strand, within IAI47_21300 at 515.940 kb on + strand, within IAI47_21300 at 515.940 kb on + strand, within IAI47_21300 at 515.940 kb on + strand, within IAI47_21300 at 515.940 kb on + strand, within IAI47_21300 at 515.940 kb on + strand, within IAI47_21300 at 515.940 kb on + strand, within IAI47_21300 at 515.941 kb on - strand, within IAI47_21300 at 515.941 kb on - strand, within IAI47_21300 at 515.941 kb on - strand, within IAI47_21300 at 516.063 kb on + strand, within IAI47_21305 at 516.064 kb on - strand, within IAI47_21305 at 516.224 kb on + strand, within IAI47_21305 at 516.366 kb on + strand, within IAI47_21305 at 516.550 kb on + strand, within IAI47_21305 at 516.550 kb on + strand, within IAI47_21305 at 516.551 kb on - strand, within IAI47_21305 at 516.551 kb on - strand, within IAI47_21305 at 516.555 kb on + strand, within IAI47_21305 at 516.556 kb on - strand, within IAI47_21305 at 516.556 kb on - strand, within IAI47_21305 at 516.556 kb on - strand, within IAI47_21305 at 516.556 kb on - strand, within IAI47_21305 at 516.599 kb on + strand at 516.600 kb on - strand at 516.600 kb on - strand at 516.603 kb on + strand at 516.604 kb on - strand at 516.665 kb on + strand at 516.674 kb on - strand at 516.675 kb on + strand at 516.675 kb on + strand at 516.764 kb on + strand at 516.773 kb on + strand at 516.773 kb on + strand at 516.774 kb on - strand at 516.774 kb on - strand at 516.774 kb on - strand at 516.774 kb on - strand at 516.774 kb on - strand at 516.787 kb on + strand at 516.787 kb on + strand at 516.788 kb on - strand at 516.830 kb on - strand, within IAI47_21310 at 516.830 kb on - strand, within IAI47_21310 at 516.857 kb on + strand, within IAI47_21310 at 516.858 kb on - strand, within IAI47_21310 at 516.858 kb on - strand, within IAI47_21310 at 516.858 kb on - strand, within IAI47_21310 at 516.858 kb on - strand, within IAI47_21310 at 516.887 kb on + strand, within IAI47_21310 at 516.933 kb on - strand, within IAI47_21310 at 516.933 kb on - strand, within IAI47_21310
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=12hrs; Outgrowth=LBkan50 remove 514,681 + -0.2 514,682 - +1.0 514,693 + +0.6 514,693 + +1.3 514,694 - -1.1 514,700 - -0.9 514,734 - -2.2 514,734 - -0.2 514,776 - -0.6 514,822 + +0.5 514,822 + +0.9 514,822 + -0.4 514,823 - +0.1 514,823 - +0.5 514,831 + -0.8 514,831 + +1.8 514,831 + -0.4 514,832 - -0.1 514,832 - -0.5 514,838 + +1.0 514,838 + +0.6 514,839 - +0.6 514,839 - +0.2 514,869 + -1.2 514,913 + IAI47_21295 0.12 +0.1 514,913 + IAI47_21295 0.12 -0.2 514,913 + IAI47_21295 0.12 -0.9 514,914 - IAI47_21295 0.12 +1.9 515,002 + IAI47_21295 0.24 +0.0 515,002 + IAI47_21295 0.24 -1.2 515,002 + IAI47_21295 0.24 -2.0 515,003 - IAI47_21295 0.24 +0.2 515,013 + IAI47_21295 0.25 +0.1 515,014 - IAI47_21295 0.26 -0.5 515,014 - IAI47_21295 0.26 -0.8 515,204 + IAI47_21295 0.52 -0.0 515,204 + IAI47_21295 0.52 +1.3 515,205 - IAI47_21295 0.52 -2.1 515,205 - IAI47_21295 0.52 -1.1 515,241 - IAI47_21295 0.57 -0.6 515,252 + IAI47_21295 0.59 +0.3 515,252 + IAI47_21295 0.59 +0.2 515,252 + IAI47_21295 0.59 +1.3 515,252 + IAI47_21295 0.59 +1.0 515,252 + IAI47_21295 0.59 -0.7 515,252 + IAI47_21295 0.59 -1.6 515,252 + IAI47_21295 0.59 +0.8 515,253 - IAI47_21295 0.59 +0.4 515,253 - IAI47_21295 0.59 -0.5 515,253 - IAI47_21295 0.59 -0.0 515,253 - IAI47_21295 0.59 +1.4 515,253 - IAI47_21295 0.59 -0.0 515,253 - IAI47_21295 0.59 +0.4 515,303 + IAI47_21295 0.66 +0.2 515,304 - IAI47_21295 0.66 +1.4 515,369 + IAI47_21295 0.75 +0.2 515,369 + IAI47_21295 0.75 +0.6 515,369 + IAI47_21295 0.75 -1.5 515,370 - IAI47_21295 0.75 +0.1 515,370 - IAI47_21295 0.75 +0.4 515,370 - IAI47_21295 0.75 +1.2 515,370 - IAI47_21295 0.75 +0.9 515,370 - IAI47_21295 0.75 -0.9 515,370 - IAI47_21295 0.75 +0.7 515,370 - IAI47_21295 0.75 +0.6 515,370 - IAI47_21295 0.75 +0.3 515,392 - IAI47_21295 0.78 +0.1 515,520 + -0.8 515,521 - +0.8 515,521 - -0.6 515,521 - +0.4 515,549 + +0.8 515,549 + +0.9 515,549 + -0.1 515,549 + -0.7 515,590 + +0.6 515,604 + +0.4 515,604 + +1.1 515,604 + -0.1 515,605 - +1.3 515,605 - +0.7 515,628 + +0.0 515,628 + -0.1 515,629 - -0.2 515,660 - -1.1 515,674 + -1.6 515,674 + -0.9 515,674 + +1.4 515,740 + IAI47_21300 0.27 -0.4 515,740 + IAI47_21300 0.27 +0.2 515,740 + IAI47_21300 0.27 +0.4 515,741 - IAI47_21300 0.27 -0.4 515,743 + IAI47_21300 0.28 -1.4 515,854 + IAI47_21300 0.62 -0.5 515,854 + IAI47_21300 0.62 -1.0 515,854 + IAI47_21300 0.62 -0.1 515,855 - IAI47_21300 0.62 -0.7 515,921 + IAI47_21300 0.83 +0.8 515,921 + IAI47_21300 0.83 -0.6 515,921 + IAI47_21300 0.83 +0.5 515,921 + IAI47_21300 0.83 -1.7 515,921 + IAI47_21300 0.83 +0.1 515,921 + IAI47_21300 0.83 -1.8 515,921 + IAI47_21300 0.83 -0.9 515,940 + IAI47_21300 0.89 +2.2 515,940 + IAI47_21300 0.89 -0.2 515,940 + IAI47_21300 0.89 -1.7 515,940 + IAI47_21300 0.89 -1.1 515,940 + IAI47_21300 0.89 -2.7 515,940 + IAI47_21300 0.89 -0.2 515,941 - IAI47_21300 0.89 -0.1 515,941 - IAI47_21300 0.89 +0.5 515,941 - IAI47_21300 0.89 -1.1 516,063 + IAI47_21305 0.13 -0.5 516,064 - IAI47_21305 0.14 +0.2 516,224 + IAI47_21305 0.38 -0.4 516,366 + IAI47_21305 0.59 +0.6 516,550 + IAI47_21305 0.86 -0.1 516,550 + IAI47_21305 0.86 -0.6 516,551 - IAI47_21305 0.86 +0.7 516,551 - IAI47_21305 0.86 -2.0 516,555 + IAI47_21305 0.87 -1.1 516,556 - IAI47_21305 0.87 -0.5 516,556 - IAI47_21305 0.87 +0.7 516,556 - IAI47_21305 0.87 +0.8 516,556 - IAI47_21305 0.87 +0.4 516,599 + +1.1 516,600 - -1.3 516,600 - +0.5 516,603 + +0.3 516,604 - +0.8 516,665 + +0.4 516,674 - -0.4 516,675 + +1.1 516,675 + -0.8 516,764 + +1.6 516,773 + +0.6 516,773 + -0.6 516,774 - -1.3 516,774 - +1.9 516,774 - +0.6 516,774 - +0.4 516,774 - +0.8 516,787 + -0.1 516,787 + +0.7 516,788 - +0.9 516,830 - IAI47_21310 0.14 -1.0 516,830 - IAI47_21310 0.14 +0.3 516,857 + IAI47_21310 0.19 +0.3 516,858 - IAI47_21310 0.19 +1.3 516,858 - IAI47_21310 0.19 +0.2 516,858 - IAI47_21310 0.19 +0.6 516,858 - IAI47_21310 0.19 +0.4 516,887 + IAI47_21310 0.24 -0.7 516,933 - IAI47_21310 0.33 +0.7 516,933 - IAI47_21310 0.33 +0.3
Or see this region's nucleotide sequence