Experiment: m.b. Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c09150 and PGA1_c09160 are separated by 3 nucleotides PGA1_c09160 and PGA1_c09170 overlap by 4 nucleotides PGA1_c09170 and PGA1_c09180 are separated by 105 nucleotides
PGA1_c09150: PGA1_c09150 - adenosylmethionine-8-amino-7-oxononanoate aminotransferase BioA, at 940,496 to 941,794
_c09150
PGA1_c09160: PGA1_c09160 - Uncharacterized protein conserved in bacteria, at 941,798 to 942,418
_c09160
PGA1_c09170: PGA1_c09170 - putative biotin synthesis protein BioC, at 942,415 to 943,203
_c09170
PGA1_c09180: PGA1_c09180 - Protein of unknown function (DUF2478)., at 943,309 to 943,836
_c09180
Position (kb)
942
943
944 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 941.427 kb on + strand, within PGA1_c09150 at 941.472 kb on - strand, within PGA1_c09150 at 941.482 kb on + strand, within PGA1_c09150 at 941.484 kb on - strand, within PGA1_c09150 at 941.504 kb on + strand, within PGA1_c09150 at 941.512 kb on - strand, within PGA1_c09150 at 941.525 kb on + strand, within PGA1_c09150 at 941.525 kb on + strand, within PGA1_c09150 at 941.528 kb on - strand, within PGA1_c09150 at 941.545 kb on + strand, within PGA1_c09150 at 941.545 kb on + strand, within PGA1_c09150 at 941.553 kb on - strand, within PGA1_c09150 at 941.559 kb on - strand, within PGA1_c09150 at 941.610 kb on + strand, within PGA1_c09150 at 941.618 kb on - strand, within PGA1_c09150 at 941.618 kb on - strand, within PGA1_c09150 at 941.707 kb on + strand at 941.715 kb on - strand at 941.715 kb on - strand at 941.758 kb on - strand at 941.788 kb on + strand at 941.814 kb on - strand at 941.820 kb on - strand at 941.825 kb on - strand at 941.931 kb on + strand, within PGA1_c09160 at 941.986 kb on - strand, within PGA1_c09160 at 942.013 kb on - strand, within PGA1_c09160 at 942.077 kb on - strand, within PGA1_c09160 at 942.077 kb on - strand, within PGA1_c09160 at 942.115 kb on + strand, within PGA1_c09160 at 942.130 kb on - strand, within PGA1_c09160 at 942.242 kb on - strand, within PGA1_c09160 at 942.249 kb on + strand, within PGA1_c09160 at 942.277 kb on + strand, within PGA1_c09160 at 942.277 kb on + strand, within PGA1_c09160 at 942.277 kb on + strand, within PGA1_c09160 at 942.285 kb on - strand, within PGA1_c09160 at 942.285 kb on - strand, within PGA1_c09160 at 942.285 kb on - strand, within PGA1_c09160 at 942.358 kb on + strand at 942.396 kb on + strand at 942.409 kb on + strand at 942.428 kb on - strand at 942.438 kb on - strand at 942.483 kb on - strand at 942.755 kb on - strand, within PGA1_c09170 at 942.756 kb on + strand, within PGA1_c09170 at 942.764 kb on + strand, within PGA1_c09170 at 942.846 kb on + strand, within PGA1_c09170 at 942.854 kb on - strand, within PGA1_c09170 at 942.860 kb on + strand, within PGA1_c09170 at 942.860 kb on + strand, within PGA1_c09170 at 942.868 kb on - strand, within PGA1_c09170 at 942.922 kb on - strand, within PGA1_c09170 at 942.943 kb on + strand, within PGA1_c09170 at 942.977 kb on + strand, within PGA1_c09170 at 943.009 kb on + strand, within PGA1_c09170 at 943.039 kb on + strand, within PGA1_c09170 at 943.047 kb on - strand, within PGA1_c09170 at 943.095 kb on - strand, within PGA1_c09170 at 943.166 kb on + strand at 943.167 kb on - strand at 943.174 kb on - strand at 943.202 kb on + strand at 943.214 kb on + strand at 943.262 kb on + strand at 943.317 kb on + strand at 943.325 kb on - strand at 943.325 kb on - strand at 943.388 kb on - strand, within PGA1_c09180 at 943.519 kb on - strand, within PGA1_c09180 at 943.559 kb on + strand, within PGA1_c09180 at 943.569 kb on + strand, within PGA1_c09180 at 943.593 kb on - strand, within PGA1_c09180 at 943.723 kb on + strand, within PGA1_c09180 at 943.788 kb on + strand at 943.794 kb on - strand at 944.043 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Dimethyl Sulfoxide 7.5 vol% remove 941,427 + PGA1_c09150 0.72 -1.2 941,472 - PGA1_c09150 0.75 +0.9 941,482 + PGA1_c09150 0.76 -0.1 941,484 - PGA1_c09150 0.76 +0.8 941,504 + PGA1_c09150 0.78 +0.1 941,512 - PGA1_c09150 0.78 +0.1 941,525 + PGA1_c09150 0.79 +0.3 941,525 + PGA1_c09150 0.79 -0.6 941,528 - PGA1_c09150 0.79 -0.4 941,545 + PGA1_c09150 0.81 -0.1 941,545 + PGA1_c09150 0.81 +0.3 941,553 - PGA1_c09150 0.81 +0.2 941,559 - PGA1_c09150 0.82 +0.4 941,610 + PGA1_c09150 0.86 +0.6 941,618 - PGA1_c09150 0.86 +0.4 941,618 - PGA1_c09150 0.86 -0.6 941,707 + +0.2 941,715 - +2.1 941,715 - +0.7 941,758 - -0.3 941,788 + +0.2 941,814 - +0.2 941,820 - -0.7 941,825 - -0.3 941,931 + PGA1_c09160 0.21 +0.5 941,986 - PGA1_c09160 0.30 -0.6 942,013 - PGA1_c09160 0.35 +0.1 942,077 - PGA1_c09160 0.45 +0.5 942,077 - PGA1_c09160 0.45 -0.6 942,115 + PGA1_c09160 0.51 -2.2 942,130 - PGA1_c09160 0.53 +0.5 942,242 - PGA1_c09160 0.71 -0.6 942,249 + PGA1_c09160 0.73 +0.1 942,277 + PGA1_c09160 0.77 +0.7 942,277 + PGA1_c09160 0.77 -0.6 942,277 + PGA1_c09160 0.77 -0.0 942,285 - PGA1_c09160 0.78 -0.7 942,285 - PGA1_c09160 0.78 -0.8 942,285 - PGA1_c09160 0.78 +0.4 942,358 + +2.2 942,396 + +0.3 942,409 + -0.9 942,428 - -0.5 942,438 - +0.8 942,483 - +0.4 942,755 - PGA1_c09170 0.43 -1.4 942,756 + PGA1_c09170 0.43 +1.7 942,764 + PGA1_c09170 0.44 +1.5 942,846 + PGA1_c09170 0.55 -1.0 942,854 - PGA1_c09170 0.56 +0.1 942,860 + PGA1_c09170 0.56 +1.0 942,860 + PGA1_c09170 0.56 +1.0 942,868 - PGA1_c09170 0.57 -0.8 942,922 - PGA1_c09170 0.64 +1.6 942,943 + PGA1_c09170 0.67 -0.2 942,977 + PGA1_c09170 0.71 +0.7 943,009 + PGA1_c09170 0.75 -0.1 943,039 + PGA1_c09170 0.79 +1.0 943,047 - PGA1_c09170 0.80 -1.4 943,095 - PGA1_c09170 0.86 +0.7 943,166 + -1.1 943,167 - -0.1 943,174 - +0.3 943,202 + +0.4 943,214 + -0.8 943,262 + +0.4 943,317 + -0.2 943,325 - +0.8 943,325 - +0.9 943,388 - PGA1_c09180 0.15 -0.1 943,519 - PGA1_c09180 0.40 +0.5 943,559 + PGA1_c09180 0.47 -0.4 943,569 + PGA1_c09180 0.49 +0.4 943,593 - PGA1_c09180 0.54 +0.5 943,723 + PGA1_c09180 0.78 -0.0 943,788 + +0.7 943,794 - +0.6 944,043 - +0.3
Or see this region's nucleotide sequence