Experiment: m.b. Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c17390 and PGA1_c17400 are separated by 191 nucleotides PGA1_c17400 and PGA1_c17410 are separated by 137 nucleotides PGA1_c17410 and PGA1_c17420 are separated by 247 nucleotides
PGA1_c17390: PGA1_c17390 - dihydrolipoyl dehydrogenase LpdA, at 1,819,088 to 1,820,482
_c17390
PGA1_c17400: PGA1_c17400 - Uncharacterized protein conserved in bacteria, at 1,820,674 to 1,821,252
_c17400
PGA1_c17410: PGA1_c17410 - MFS type transporter, at 1,821,390 to 1,822,664
_c17410
PGA1_c17420: PGA1_c17420 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase QueA, at 1,822,912 to 1,823,982
_c17420
Position (kb)
1821
1822
1823 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1820.624 kb on + strand at 1820.671 kb on + strand at 1820.742 kb on + strand, within PGA1_c17400 at 1820.845 kb on + strand, within PGA1_c17400 at 1821.074 kb on - strand, within PGA1_c17400 at 1821.074 kb on - strand, within PGA1_c17400 at 1821.076 kb on + strand, within PGA1_c17400 at 1821.076 kb on + strand, within PGA1_c17400 at 1821.084 kb on - strand, within PGA1_c17400 at 1821.194 kb on - strand, within PGA1_c17400 at 1821.207 kb on - strand at 1821.207 kb on - strand at 1821.207 kb on - strand at 1821.237 kb on + strand at 1821.237 kb on + strand at 1821.245 kb on - strand at 1821.247 kb on + strand at 1821.247 kb on + strand at 1821.262 kb on - strand at 1821.274 kb on - strand at 1821.274 kb on - strand at 1821.311 kb on - strand at 1821.456 kb on + strand at 1821.470 kb on - strand at 1821.476 kb on - strand at 1821.490 kb on + strand at 1821.531 kb on - strand, within PGA1_c17410 at 1821.677 kb on + strand, within PGA1_c17410 at 1821.737 kb on - strand, within PGA1_c17410 at 1821.765 kb on - strand, within PGA1_c17410 at 1821.765 kb on - strand, within PGA1_c17410 at 1821.766 kb on - strand, within PGA1_c17410 at 1821.775 kb on + strand, within PGA1_c17410 at 1821.849 kb on - strand, within PGA1_c17410 at 1821.854 kb on - strand, within PGA1_c17410 at 1821.854 kb on - strand, within PGA1_c17410 at 1821.854 kb on - strand, within PGA1_c17410 at 1821.934 kb on + strand, within PGA1_c17410 at 1822.023 kb on + strand, within PGA1_c17410 at 1822.046 kb on + strand, within PGA1_c17410 at 1822.051 kb on + strand, within PGA1_c17410 at 1822.056 kb on + strand, within PGA1_c17410 at 1822.056 kb on + strand, within PGA1_c17410 at 1822.056 kb on + strand, within PGA1_c17410 at 1822.064 kb on - strand, within PGA1_c17410 at 1822.064 kb on - strand, within PGA1_c17410 at 1822.104 kb on - strand, within PGA1_c17410 at 1822.109 kb on - strand, within PGA1_c17410 at 1822.114 kb on - strand, within PGA1_c17410 at 1822.223 kb on + strand, within PGA1_c17410 at 1822.245 kb on + strand, within PGA1_c17410 at 1822.255 kb on + strand, within PGA1_c17410 at 1822.255 kb on + strand, within PGA1_c17410 at 1822.255 kb on + strand, within PGA1_c17410 at 1822.255 kb on + strand, within PGA1_c17410 at 1822.263 kb on - strand, within PGA1_c17410 at 1822.326 kb on + strand, within PGA1_c17410 at 1822.380 kb on - strand, within PGA1_c17410 at 1822.397 kb on - strand, within PGA1_c17410 at 1822.431 kb on + strand, within PGA1_c17410 at 1822.486 kb on + strand, within PGA1_c17410 at 1822.554 kb on + strand at 1822.562 kb on - strand at 1822.590 kb on + strand at 1822.632 kb on - strand at 1822.707 kb on + strand at 1822.713 kb on + strand at 1822.725 kb on + strand at 1822.733 kb on - strand at 1822.733 kb on - strand at 1822.837 kb on - strand at 1822.867 kb on + strand at 1822.873 kb on - strand at 1822.875 kb on - strand at 1822.894 kb on - strand at 1822.927 kb on - strand at 1823.149 kb on + strand, within PGA1_c17420 at 1823.375 kb on + strand, within PGA1_c17420 at 1823.375 kb on + strand, within PGA1_c17420 at 1823.383 kb on - strand, within PGA1_c17420 at 1823.383 kb on - strand, within PGA1_c17420 at 1823.383 kb on - strand, within PGA1_c17420 at 1823.593 kb on + strand, within PGA1_c17420 at 1823.593 kb on + strand, within PGA1_c17420
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Dimethyl Sulfoxide 7.5 vol% remove 1,820,624 + +0.5 1,820,671 + -0.2 1,820,742 + PGA1_c17400 0.12 +1.2 1,820,845 + PGA1_c17400 0.30 -0.5 1,821,074 - PGA1_c17400 0.69 -0.1 1,821,074 - PGA1_c17400 0.69 -0.5 1,821,076 + PGA1_c17400 0.69 +0.0 1,821,076 + PGA1_c17400 0.69 +0.5 1,821,084 - PGA1_c17400 0.71 -0.2 1,821,194 - PGA1_c17400 0.90 -0.5 1,821,207 - -0.8 1,821,207 - +0.4 1,821,207 - +0.3 1,821,237 + +2.0 1,821,237 + -0.8 1,821,245 - +0.8 1,821,247 + -0.3 1,821,247 + -0.6 1,821,262 - +0.5 1,821,274 - +0.0 1,821,274 - -1.3 1,821,311 - +0.4 1,821,456 + -0.1 1,821,470 - -1.5 1,821,476 - -0.1 1,821,490 + -1.1 1,821,531 - PGA1_c17410 0.11 -0.8 1,821,677 + PGA1_c17410 0.23 +0.8 1,821,737 - PGA1_c17410 0.27 +0.0 1,821,765 - PGA1_c17410 0.29 -0.5 1,821,765 - PGA1_c17410 0.29 +0.4 1,821,766 - PGA1_c17410 0.29 +0.9 1,821,775 + PGA1_c17410 0.30 +0.8 1,821,849 - PGA1_c17410 0.36 -0.0 1,821,854 - PGA1_c17410 0.36 +0.2 1,821,854 - PGA1_c17410 0.36 +1.3 1,821,854 - PGA1_c17410 0.36 +0.2 1,821,934 + PGA1_c17410 0.43 +0.5 1,822,023 + PGA1_c17410 0.50 +0.1 1,822,046 + PGA1_c17410 0.51 -1.6 1,822,051 + PGA1_c17410 0.52 +0.9 1,822,056 + PGA1_c17410 0.52 +0.1 1,822,056 + PGA1_c17410 0.52 -0.2 1,822,056 + PGA1_c17410 0.52 +0.7 1,822,064 - PGA1_c17410 0.53 -1.7 1,822,064 - PGA1_c17410 0.53 -0.5 1,822,104 - PGA1_c17410 0.56 -0.0 1,822,109 - PGA1_c17410 0.56 -0.1 1,822,114 - PGA1_c17410 0.57 +1.6 1,822,223 + PGA1_c17410 0.65 +0.1 1,822,245 + PGA1_c17410 0.67 +0.6 1,822,255 + PGA1_c17410 0.68 +0.5 1,822,255 + PGA1_c17410 0.68 +0.4 1,822,255 + PGA1_c17410 0.68 -0.0 1,822,255 + PGA1_c17410 0.68 -1.2 1,822,263 - PGA1_c17410 0.68 +0.3 1,822,326 + PGA1_c17410 0.73 +0.1 1,822,380 - PGA1_c17410 0.78 +1.2 1,822,397 - PGA1_c17410 0.79 +1.0 1,822,431 + PGA1_c17410 0.82 -0.4 1,822,486 + PGA1_c17410 0.86 +1.2 1,822,554 + +0.4 1,822,562 - -2.0 1,822,590 + +0.8 1,822,632 - -0.3 1,822,707 + +0.0 1,822,713 + +0.5 1,822,725 + +0.5 1,822,733 - -0.2 1,822,733 - -0.4 1,822,837 - +0.3 1,822,867 + +0.2 1,822,873 - -0.2 1,822,875 - +0.4 1,822,894 - +0.0 1,822,927 - +0.3 1,823,149 + PGA1_c17420 0.22 -1.6 1,823,375 + PGA1_c17420 0.43 -1.2 1,823,375 + PGA1_c17420 0.43 -0.9 1,823,383 - PGA1_c17420 0.44 -0.6 1,823,383 - PGA1_c17420 0.44 -0.3 1,823,383 - PGA1_c17420 0.44 -0.1 1,823,593 + PGA1_c17420 0.64 -0.5 1,823,593 + PGA1_c17420 0.64 -1.2
Or see this region's nucleotide sequence