Experiment: m.b. Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c13160 and PGA1_c13170 are separated by 29 nucleotides PGA1_c13170 and PGA1_c13180 overlap by 4 nucleotides
PGA1_c13160: PGA1_c13160 - mannitol 2-dehydrogenase MtlK, at 1,361,569 to 1,363,035
_c13160
PGA1_c13170: PGA1_c13170 - Sorbitol dehydrogenase (EC 1.1.1.14) (from data) , at 1,363,065 to 1,363,838
_c13170
PGA1_c13180: PGA1_c13180 - ABC transporter for D-Sorbitol, ATPase component (from data) , at 1,363,835 to 1,364,839
_c13180
Position (kb)
1363
1364 Strain fitness (log2 ratio)
-1
0
1 at 1362.130 kb on + strand, within PGA1_c13160 at 1362.172 kb on + strand, within PGA1_c13160 at 1362.208 kb on + strand, within PGA1_c13160 at 1362.208 kb on + strand, within PGA1_c13160 at 1362.216 kb on - strand, within PGA1_c13160 at 1362.216 kb on - strand, within PGA1_c13160 at 1362.251 kb on - strand, within PGA1_c13160 at 1362.304 kb on - strand, within PGA1_c13160 at 1362.304 kb on - strand, within PGA1_c13160 at 1362.304 kb on - strand, within PGA1_c13160 at 1362.429 kb on + strand, within PGA1_c13160 at 1362.429 kb on + strand, within PGA1_c13160 at 1362.445 kb on + strand, within PGA1_c13160 at 1362.466 kb on - strand, within PGA1_c13160 at 1362.483 kb on - strand, within PGA1_c13160 at 1362.491 kb on - strand, within PGA1_c13160 at 1362.506 kb on - strand, within PGA1_c13160 at 1362.582 kb on + strand, within PGA1_c13160 at 1362.608 kb on + strand, within PGA1_c13160 at 1362.608 kb on + strand, within PGA1_c13160 at 1362.608 kb on + strand, within PGA1_c13160 at 1362.608 kb on + strand, within PGA1_c13160 at 1362.608 kb on + strand, within PGA1_c13160 at 1362.610 kb on + strand, within PGA1_c13160 at 1362.616 kb on - strand, within PGA1_c13160 at 1362.616 kb on - strand, within PGA1_c13160 at 1362.616 kb on - strand, within PGA1_c13160 at 1362.650 kb on - strand, within PGA1_c13160 at 1362.674 kb on - strand, within PGA1_c13160 at 1362.674 kb on - strand, within PGA1_c13160 at 1362.708 kb on - strand, within PGA1_c13160 at 1362.710 kb on + strand, within PGA1_c13160 at 1362.752 kb on + strand, within PGA1_c13160 at 1362.755 kb on - strand, within PGA1_c13160 at 1362.796 kb on + strand, within PGA1_c13160 at 1362.885 kb on + strand, within PGA1_c13160 at 1362.915 kb on - strand at 1362.969 kb on - strand at 1362.981 kb on - strand at 1362.990 kb on - strand at 1362.995 kb on + strand at 1363.052 kb on - strand at 1363.057 kb on - strand at 1363.098 kb on + strand at 1363.098 kb on + strand at 1363.098 kb on + strand at 1363.106 kb on - strand at 1363.106 kb on - strand at 1363.106 kb on - strand at 1363.107 kb on - strand at 1363.107 kb on - strand at 1363.131 kb on + strand at 1363.160 kb on + strand, within PGA1_c13170 at 1363.176 kb on - strand, within PGA1_c13170 at 1363.225 kb on + strand, within PGA1_c13170 at 1363.263 kb on + strand, within PGA1_c13170 at 1363.263 kb on + strand, within PGA1_c13170 at 1363.271 kb on - strand, within PGA1_c13170 at 1363.271 kb on - strand, within PGA1_c13170 at 1363.271 kb on - strand, within PGA1_c13170 at 1363.342 kb on - strand, within PGA1_c13170 at 1363.351 kb on + strand, within PGA1_c13170 at 1363.444 kb on + strand, within PGA1_c13170 at 1363.472 kb on - strand, within PGA1_c13170 at 1363.543 kb on + strand, within PGA1_c13170 at 1363.551 kb on - strand, within PGA1_c13170 at 1363.569 kb on - strand, within PGA1_c13170 at 1363.652 kb on - strand, within PGA1_c13170 at 1363.652 kb on - strand, within PGA1_c13170 at 1363.699 kb on + strand, within PGA1_c13170 at 1363.707 kb on - strand, within PGA1_c13170 at 1363.714 kb on - strand, within PGA1_c13170 at 1363.801 kb on + strand at 1363.801 kb on + strand at 1363.801 kb on + strand at 1363.801 kb on + strand at 1363.801 kb on + strand at 1363.801 kb on + strand at 1363.801 kb on + strand at 1363.809 kb on - strand at 1363.809 kb on - strand at 1363.822 kb on + strand at 1363.890 kb on - strand at 1364.093 kb on - strand, within PGA1_c13180 at 1364.098 kb on - strand, within PGA1_c13180 at 1364.169 kb on + strand, within PGA1_c13180 at 1364.190 kb on + strand, within PGA1_c13180 at 1364.190 kb on + strand, within PGA1_c13180 at 1364.258 kb on - strand, within PGA1_c13180 at 1364.349 kb on + strand, within PGA1_c13180 at 1364.433 kb on + strand, within PGA1_c13180 at 1364.433 kb on + strand, within PGA1_c13180 at 1364.436 kb on - strand, within PGA1_c13180 at 1364.441 kb on - strand, within PGA1_c13180 at 1364.441 kb on - strand, within PGA1_c13180 at 1364.482 kb on - strand, within PGA1_c13180 at 1364.665 kb on + strand, within PGA1_c13180 at 1364.681 kb on + strand, within PGA1_c13180 at 1364.823 kb on + strand at 1364.831 kb on - strand at 1364.831 kb on - strand at 1364.833 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Dimethyl Sulfoxide 7.5 vol% remove 1,362,130 + PGA1_c13160 0.38 +0.4 1,362,172 + PGA1_c13160 0.41 +0.4 1,362,208 + PGA1_c13160 0.44 -0.8 1,362,208 + PGA1_c13160 0.44 +0.4 1,362,216 - PGA1_c13160 0.44 +1.2 1,362,216 - PGA1_c13160 0.44 -0.1 1,362,251 - PGA1_c13160 0.46 +1.7 1,362,304 - PGA1_c13160 0.50 -0.2 1,362,304 - PGA1_c13160 0.50 -0.6 1,362,304 - PGA1_c13160 0.50 -0.3 1,362,429 + PGA1_c13160 0.59 -1.4 1,362,429 + PGA1_c13160 0.59 +0.6 1,362,445 + PGA1_c13160 0.60 -0.0 1,362,466 - PGA1_c13160 0.61 +0.2 1,362,483 - PGA1_c13160 0.62 -0.3 1,362,491 - PGA1_c13160 0.63 -0.5 1,362,506 - PGA1_c13160 0.64 -0.5 1,362,582 + PGA1_c13160 0.69 -0.1 1,362,608 + PGA1_c13160 0.71 +0.3 1,362,608 + PGA1_c13160 0.71 -0.8 1,362,608 + PGA1_c13160 0.71 -0.1 1,362,608 + PGA1_c13160 0.71 +0.7 1,362,608 + PGA1_c13160 0.71 -0.3 1,362,610 + PGA1_c13160 0.71 -0.7 1,362,616 - PGA1_c13160 0.71 -0.1 1,362,616 - PGA1_c13160 0.71 -0.4 1,362,616 - PGA1_c13160 0.71 +0.0 1,362,650 - PGA1_c13160 0.74 -0.8 1,362,674 - PGA1_c13160 0.75 +0.5 1,362,674 - PGA1_c13160 0.75 +0.2 1,362,708 - PGA1_c13160 0.78 +0.8 1,362,710 + PGA1_c13160 0.78 -0.2 1,362,752 + PGA1_c13160 0.81 +0.7 1,362,755 - PGA1_c13160 0.81 -1.2 1,362,796 + PGA1_c13160 0.84 +0.2 1,362,885 + PGA1_c13160 0.90 -0.1 1,362,915 - -1.1 1,362,969 - -0.3 1,362,981 - -0.4 1,362,990 - -0.4 1,362,995 + -1.0 1,363,052 - +0.4 1,363,057 - +0.3 1,363,098 + +0.3 1,363,098 + +0.8 1,363,098 + +0.1 1,363,106 - +0.7 1,363,106 - -0.8 1,363,106 - +1.4 1,363,107 - +0.7 1,363,107 - -0.5 1,363,131 + +0.3 1,363,160 + PGA1_c13170 0.12 +1.0 1,363,176 - PGA1_c13170 0.14 -1.0 1,363,225 + PGA1_c13170 0.21 +0.8 1,363,263 + PGA1_c13170 0.26 -0.4 1,363,263 + PGA1_c13170 0.26 +1.7 1,363,271 - PGA1_c13170 0.27 +0.8 1,363,271 - PGA1_c13170 0.27 +0.3 1,363,271 - PGA1_c13170 0.27 +0.1 1,363,342 - PGA1_c13170 0.36 +0.5 1,363,351 + PGA1_c13170 0.37 -1.0 1,363,444 + PGA1_c13170 0.49 +0.6 1,363,472 - PGA1_c13170 0.53 +1.4 1,363,543 + PGA1_c13170 0.62 -0.3 1,363,551 - PGA1_c13170 0.63 +1.1 1,363,569 - PGA1_c13170 0.65 +0.2 1,363,652 - PGA1_c13170 0.76 +0.8 1,363,652 - PGA1_c13170 0.76 +1.1 1,363,699 + PGA1_c13170 0.82 +0.5 1,363,707 - PGA1_c13170 0.83 -0.3 1,363,714 - PGA1_c13170 0.84 +0.9 1,363,801 + +0.4 1,363,801 + +0.8 1,363,801 + -0.2 1,363,801 + +0.9 1,363,801 + +0.3 1,363,801 + -0.3 1,363,801 + -0.1 1,363,809 - +0.6 1,363,809 - -0.0 1,363,822 + +0.1 1,363,890 - -0.6 1,364,093 - PGA1_c13180 0.26 +0.8 1,364,098 - PGA1_c13180 0.26 -0.1 1,364,169 + PGA1_c13180 0.33 +0.1 1,364,190 + PGA1_c13180 0.35 -0.6 1,364,190 + PGA1_c13180 0.35 +0.5 1,364,258 - PGA1_c13180 0.42 +0.4 1,364,349 + PGA1_c13180 0.51 -0.3 1,364,433 + PGA1_c13180 0.60 -0.7 1,364,433 + PGA1_c13180 0.60 +0.7 1,364,436 - PGA1_c13180 0.60 +0.0 1,364,441 - PGA1_c13180 0.60 +0.2 1,364,441 - PGA1_c13180 0.60 -0.7 1,364,482 - PGA1_c13180 0.64 +0.1 1,364,665 + PGA1_c13180 0.83 -0.0 1,364,681 + PGA1_c13180 0.84 -0.0 1,364,823 + -0.5 1,364,831 - -0.3 1,364,831 - -0.5 1,364,833 + +0.0
Or see this region's nucleotide sequence