Experiment: m.b. Fusidic 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c11510 and PGA1_c11520 are separated by 115 nucleotides PGA1_c11520 and PGA1_c11530 overlap by 1 nucleotides PGA1_c11530 and PGA1_c11540 are separated by 93 nucleotides
PGA1_c11510: PGA1_c11510 - gamma-glutamylputrescine oxidoreductase PuuB, at 1,189,840 to 1,191,150
_c11510
PGA1_c11520: PGA1_c11520 - gamma-glutamylputrescine synthetase PuuA, at 1,191,266 to 1,192,624
_c11520
PGA1_c11530: PGA1_c11530 - putative glutamine amidotransferase class I, at 1,192,624 to 1,193,304
_c11530
PGA1_c11540: PGA1_c11540 - gamma-glutamylputrescine synthetase PuuA, at 1,193,398 to 1,194,744
_c11540
Position (kb)
1191
1192
1193 Strain fitness (log2 ratio)
-2
-1
0
1 at 1190.363 kb on + strand, within PGA1_c11510 at 1190.496 kb on - strand, within PGA1_c11510 at 1190.548 kb on + strand, within PGA1_c11510 at 1190.560 kb on + strand, within PGA1_c11510 at 1190.642 kb on + strand, within PGA1_c11510 at 1190.642 kb on + strand, within PGA1_c11510 at 1190.650 kb on - strand, within PGA1_c11510 at 1190.696 kb on + strand, within PGA1_c11510 at 1190.745 kb on - strand, within PGA1_c11510 at 1191.040 kb on + strand at 1191.040 kb on + strand at 1191.123 kb on + strand at 1191.157 kb on + strand at 1191.238 kb on - strand at 1191.283 kb on + strand at 1191.364 kb on + strand at 1191.371 kb on + strand at 1191.378 kb on + strand at 1191.398 kb on - strand at 1191.424 kb on - strand, within PGA1_c11520 at 1191.436 kb on + strand, within PGA1_c11520 at 1191.436 kb on - strand, within PGA1_c11520 at 1191.465 kb on + strand, within PGA1_c11520 at 1191.465 kb on + strand, within PGA1_c11520 at 1191.541 kb on + strand, within PGA1_c11520 at 1191.548 kb on - strand, within PGA1_c11520 at 1191.877 kb on - strand, within PGA1_c11520 at 1191.928 kb on + strand, within PGA1_c11520 at 1191.928 kb on - strand, within PGA1_c11520 at 1192.012 kb on + strand, within PGA1_c11520 at 1192.014 kb on - strand, within PGA1_c11520 at 1192.034 kb on + strand, within PGA1_c11520 at 1192.034 kb on + strand, within PGA1_c11520 at 1192.034 kb on + strand, within PGA1_c11520 at 1192.034 kb on + strand, within PGA1_c11520 at 1192.034 kb on + strand, within PGA1_c11520 at 1192.042 kb on - strand, within PGA1_c11520 at 1192.101 kb on + strand, within PGA1_c11520 at 1192.167 kb on + strand, within PGA1_c11520 at 1192.168 kb on + strand, within PGA1_c11520 at 1192.234 kb on - strand, within PGA1_c11520 at 1192.241 kb on + strand, within PGA1_c11520 at 1192.244 kb on - strand, within PGA1_c11520 at 1192.259 kb on - strand, within PGA1_c11520 at 1192.281 kb on - strand, within PGA1_c11520 at 1192.349 kb on - strand, within PGA1_c11520 at 1192.365 kb on + strand, within PGA1_c11520 at 1192.509 kb on + strand at 1192.517 kb on - strand at 1192.524 kb on + strand at 1192.583 kb on + strand at 1192.591 kb on - strand at 1192.591 kb on - strand at 1192.593 kb on + strand at 1192.620 kb on + strand at 1192.693 kb on - strand, within PGA1_c11530 at 1192.736 kb on - strand, within PGA1_c11530 at 1192.802 kb on + strand, within PGA1_c11530 at 1192.802 kb on + strand, within PGA1_c11530 at 1192.802 kb on + strand, within PGA1_c11530 at 1192.802 kb on + strand, within PGA1_c11530 at 1192.802 kb on + strand, within PGA1_c11530 at 1192.802 kb on + strand, within PGA1_c11530 at 1192.802 kb on + strand, within PGA1_c11530 at 1192.802 kb on - strand, within PGA1_c11530 at 1192.810 kb on - strand, within PGA1_c11530 at 1192.810 kb on - strand, within PGA1_c11530 at 1192.810 kb on - strand, within PGA1_c11530 at 1192.810 kb on - strand, within PGA1_c11530 at 1192.879 kb on - strand, within PGA1_c11530 at 1192.879 kb on - strand, within PGA1_c11530 at 1192.883 kb on + strand, within PGA1_c11530 at 1192.958 kb on + strand, within PGA1_c11530 at 1193.001 kb on + strand, within PGA1_c11530 at 1193.016 kb on - strand, within PGA1_c11530 at 1193.126 kb on + strand, within PGA1_c11530 at 1193.189 kb on - strand, within PGA1_c11530 at 1193.189 kb on - strand, within PGA1_c11530 at 1193.263 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Fusidic 0.0002 mg/ml remove 1,190,363 + PGA1_c11510 0.40 -1.4 1,190,496 - PGA1_c11510 0.50 -0.5 1,190,548 + PGA1_c11510 0.54 -1.2 1,190,560 + PGA1_c11510 0.55 +0.2 1,190,642 + PGA1_c11510 0.61 +0.1 1,190,642 + PGA1_c11510 0.61 -0.1 1,190,650 - PGA1_c11510 0.62 -1.2 1,190,696 + PGA1_c11510 0.65 +0.1 1,190,745 - PGA1_c11510 0.69 -0.1 1,191,040 + +0.6 1,191,040 + +0.2 1,191,123 + -0.2 1,191,157 + -1.2 1,191,238 - +0.0 1,191,283 + -0.9 1,191,364 + -1.6 1,191,371 + -0.7 1,191,378 + -0.2 1,191,398 - -1.6 1,191,424 - PGA1_c11520 0.12 -0.3 1,191,436 + PGA1_c11520 0.13 -0.1 1,191,436 - PGA1_c11520 0.13 -0.0 1,191,465 + PGA1_c11520 0.15 +0.5 1,191,465 + PGA1_c11520 0.15 -1.2 1,191,541 + PGA1_c11520 0.20 -0.6 1,191,548 - PGA1_c11520 0.21 -0.0 1,191,877 - PGA1_c11520 0.45 +0.3 1,191,928 + PGA1_c11520 0.49 -2.2 1,191,928 - PGA1_c11520 0.49 +0.9 1,192,012 + PGA1_c11520 0.55 -0.1 1,192,014 - PGA1_c11520 0.55 -0.2 1,192,034 + PGA1_c11520 0.57 -0.6 1,192,034 + PGA1_c11520 0.57 +0.8 1,192,034 + PGA1_c11520 0.57 -1.2 1,192,034 + PGA1_c11520 0.57 -1.0 1,192,034 + PGA1_c11520 0.57 -0.8 1,192,042 - PGA1_c11520 0.57 -1.4 1,192,101 + PGA1_c11520 0.61 -0.7 1,192,167 + PGA1_c11520 0.66 +0.2 1,192,168 + PGA1_c11520 0.66 -0.1 1,192,234 - PGA1_c11520 0.71 +0.8 1,192,241 + PGA1_c11520 0.72 -0.7 1,192,244 - PGA1_c11520 0.72 -0.1 1,192,259 - PGA1_c11520 0.73 -1.1 1,192,281 - PGA1_c11520 0.75 -0.9 1,192,349 - PGA1_c11520 0.80 +0.1 1,192,365 + PGA1_c11520 0.81 -1.2 1,192,509 + -1.3 1,192,517 - +0.4 1,192,524 + -2.1 1,192,583 + -0.1 1,192,591 - -0.5 1,192,591 - -2.4 1,192,593 + -0.6 1,192,620 + -0.8 1,192,693 - PGA1_c11530 0.10 +0.7 1,192,736 - PGA1_c11530 0.16 +0.4 1,192,802 + PGA1_c11530 0.26 -0.3 1,192,802 + PGA1_c11530 0.26 +1.7 1,192,802 + PGA1_c11530 0.26 -0.1 1,192,802 + PGA1_c11530 0.26 -0.4 1,192,802 + PGA1_c11530 0.26 +0.1 1,192,802 + PGA1_c11530 0.26 -1.5 1,192,802 + PGA1_c11530 0.26 +1.3 1,192,802 - PGA1_c11530 0.26 +0.5 1,192,810 - PGA1_c11530 0.27 +1.0 1,192,810 - PGA1_c11530 0.27 -0.8 1,192,810 - PGA1_c11530 0.27 -0.9 1,192,810 - PGA1_c11530 0.27 +0.4 1,192,879 - PGA1_c11530 0.37 +0.3 1,192,879 - PGA1_c11530 0.37 +0.3 1,192,883 + PGA1_c11530 0.38 +0.7 1,192,958 + PGA1_c11530 0.49 -0.7 1,193,001 + PGA1_c11530 0.55 -0.3 1,193,016 - PGA1_c11530 0.58 -0.3 1,193,126 + PGA1_c11530 0.74 -0.1 1,193,189 - PGA1_c11530 0.83 -0.5 1,193,189 - PGA1_c11530 0.83 -0.8 1,193,263 + -0.8
Or see this region's nucleotide sequence