Experiment: m.b. Phosphomycin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c09030 and PGA1_c09040 overlap by 4 nucleotides PGA1_c09040 and PGA1_c09050 overlap by 25 nucleotides PGA1_c09050 and PGA1_c09070 are separated by 23 nucleotides PGA1_c09070 and PGA1_c09090 are separated by 3 nucleotides PGA1_c09090 and PGA1_c09100 overlap by 32 nucleotides
PGA1_c09030: PGA1_c09030 - phosphomethylpyrimidine kinase ThiD, at 931,201 to 931,998
_c09030
PGA1_c09040: PGA1_c09040 - putative adenylyltransferase ThiF, at 931,995 to 932,984
_c09040
PGA1_c09050: PGA1_c09050 - thiamine-phosphate pyrophosphorylase ThiE, at 932,960 to 933,553
_c09050
PGA1_c09070: PGA1_c09070 - thiazole biosynthesis protein ThiG, at 933,577 to 934,344
_c09070
PGA1_c09090: PGA1_c09090 - thiamine biosynthesis protein ThiS, at 934,348 to 934,545
_c09090
PGA1_c09100: PGA1_c09100 - putative thiamine biosynthesis oxidoreductase ThiO, at 934,514 to 935,488
_c09100
Position (kb)
932
933
934 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 931.996 kb on + strand at 932.004 kb on - strand at 932.004 kb on - strand at 932.009 kb on - strand at 932.016 kb on + strand at 932.068 kb on - strand at 932.091 kb on + strand at 932.091 kb on + strand at 932.091 kb on + strand at 932.099 kb on - strand, within PGA1_c09040 at 932.099 kb on - strand, within PGA1_c09040 at 932.099 kb on - strand, within PGA1_c09040 at 932.099 kb on - strand, within PGA1_c09040 at 932.099 kb on - strand, within PGA1_c09040 at 932.184 kb on - strand, within PGA1_c09040 at 932.207 kb on - strand, within PGA1_c09040 at 932.222 kb on - strand, within PGA1_c09040 at 932.267 kb on + strand, within PGA1_c09040 at 932.267 kb on + strand, within PGA1_c09040 at 932.275 kb on - strand, within PGA1_c09040 at 932.353 kb on - strand, within PGA1_c09040 at 932.436 kb on + strand, within PGA1_c09040 at 932.444 kb on - strand, within PGA1_c09040 at 932.496 kb on + strand, within PGA1_c09040 at 932.541 kb on - strand, within PGA1_c09040 at 932.576 kb on + strand, within PGA1_c09040 at 932.576 kb on + strand, within PGA1_c09040 at 932.578 kb on + strand, within PGA1_c09040 at 932.578 kb on + strand, within PGA1_c09040 at 932.578 kb on + strand, within PGA1_c09040 at 932.578 kb on + strand, within PGA1_c09040 at 932.580 kb on + strand, within PGA1_c09040 at 932.584 kb on - strand, within PGA1_c09040 at 932.586 kb on - strand, within PGA1_c09040 at 932.586 kb on - strand, within PGA1_c09040 at 932.586 kb on - strand, within PGA1_c09040 at 932.588 kb on + strand, within PGA1_c09040 at 932.588 kb on + strand, within PGA1_c09040 at 932.596 kb on - strand, within PGA1_c09040 at 932.708 kb on + strand, within PGA1_c09040 at 932.722 kb on + strand, within PGA1_c09040 at 932.757 kb on + strand, within PGA1_c09040 at 932.760 kb on - strand, within PGA1_c09040 at 932.786 kb on - strand, within PGA1_c09040 at 932.789 kb on + strand, within PGA1_c09040 at 932.800 kb on + strand, within PGA1_c09040 at 932.849 kb on + strand, within PGA1_c09040 at 932.859 kb on + strand, within PGA1_c09040 at 932.859 kb on + strand, within PGA1_c09040 at 932.862 kb on - strand, within PGA1_c09040 at 932.981 kb on - strand at 932.981 kb on - strand at 932.981 kb on - strand at 933.114 kb on + strand, within PGA1_c09050 at 933.146 kb on + strand, within PGA1_c09050 at 933.154 kb on - strand, within PGA1_c09050 at 933.184 kb on - strand, within PGA1_c09050 at 933.186 kb on + strand, within PGA1_c09050 at 933.186 kb on + strand, within PGA1_c09050 at 933.213 kb on - strand, within PGA1_c09050 at 933.275 kb on + strand, within PGA1_c09050 at 933.283 kb on - strand, within PGA1_c09050 at 933.283 kb on - strand, within PGA1_c09050 at 933.312 kb on - strand, within PGA1_c09050 at 933.314 kb on + strand, within PGA1_c09050 at 933.314 kb on + strand, within PGA1_c09050 at 933.322 kb on - strand, within PGA1_c09050 at 933.322 kb on - strand, within PGA1_c09050 at 933.484 kb on + strand, within PGA1_c09050 at 933.488 kb on + strand, within PGA1_c09050 at 933.518 kb on + strand at 933.575 kb on + strand at 933.580 kb on + strand at 933.581 kb on - strand at 933.583 kb on - strand at 933.583 kb on - strand at 933.583 kb on - strand at 933.583 kb on - strand at 933.603 kb on - strand at 933.689 kb on + strand, within PGA1_c09070 at 933.702 kb on - strand, within PGA1_c09070 at 933.731 kb on + strand, within PGA1_c09070 at 933.731 kb on + strand, within PGA1_c09070 at 933.739 kb on - strand, within PGA1_c09070 at 933.767 kb on + strand, within PGA1_c09070 at 933.862 kb on + strand, within PGA1_c09070 at 933.865 kb on - strand, within PGA1_c09070 at 933.878 kb on + strand, within PGA1_c09070 at 933.881 kb on - strand, within PGA1_c09070 at 933.901 kb on - strand, within PGA1_c09070 at 933.948 kb on + strand, within PGA1_c09070 at 933.972 kb on + strand, within PGA1_c09070 at 933.978 kb on - strand, within PGA1_c09070 at 933.980 kb on - strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.076 kb on + strand, within PGA1_c09070 at 934.084 kb on - strand, within PGA1_c09070 at 934.103 kb on - strand, within PGA1_c09070 at 934.103 kb on - strand, within PGA1_c09070 at 934.108 kb on + strand, within PGA1_c09070 at 934.142 kb on + strand, within PGA1_c09070 at 934.142 kb on + strand, within PGA1_c09070 at 934.150 kb on - strand, within PGA1_c09070 at 934.152 kb on + strand, within PGA1_c09070 at 934.152 kb on + strand, within PGA1_c09070 at 934.162 kb on + strand, within PGA1_c09070 at 934.275 kb on + strand at 934.275 kb on - strand at 934.306 kb on - strand at 934.311 kb on + strand at 934.311 kb on + strand at 934.311 kb on + strand at 934.319 kb on - strand at 934.339 kb on - strand at 934.354 kb on - strand at 934.364 kb on + strand at 934.472 kb on - strand, within PGA1_c09090 at 934.513 kb on - strand, within PGA1_c09090 at 934.529 kb on - strand at 934.539 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Phosphomycin 2 mg/ml remove 931,996 + +0.1 932,004 - -0.6 932,004 - +0.2 932,009 - +0.8 932,016 + +0.8 932,068 - +1.4 932,091 + +0.5 932,091 + +0.9 932,091 + +1.0 932,099 - PGA1_c09040 0.11 +0.1 932,099 - PGA1_c09040 0.11 +1.5 932,099 - PGA1_c09040 0.11 +1.7 932,099 - PGA1_c09040 0.11 +0.7 932,099 - PGA1_c09040 0.11 +1.1 932,184 - PGA1_c09040 0.19 +1.6 932,207 - PGA1_c09040 0.21 +1.1 932,222 - PGA1_c09040 0.23 +0.9 932,267 + PGA1_c09040 0.27 +0.9 932,267 + PGA1_c09040 0.27 +0.7 932,275 - PGA1_c09040 0.28 +0.1 932,353 - PGA1_c09040 0.36 -0.1 932,436 + PGA1_c09040 0.45 -0.0 932,444 - PGA1_c09040 0.45 +0.2 932,496 + PGA1_c09040 0.51 +1.1 932,541 - PGA1_c09040 0.55 -0.3 932,576 + PGA1_c09040 0.59 +1.5 932,576 + PGA1_c09040 0.59 +1.4 932,578 + PGA1_c09040 0.59 -0.2 932,578 + PGA1_c09040 0.59 +1.0 932,578 + PGA1_c09040 0.59 +0.8 932,578 + PGA1_c09040 0.59 -0.2 932,580 + PGA1_c09040 0.59 +1.2 932,584 - PGA1_c09040 0.59 +1.0 932,586 - PGA1_c09040 0.60 +0.6 932,586 - PGA1_c09040 0.60 +0.5 932,586 - PGA1_c09040 0.60 +0.3 932,588 + PGA1_c09040 0.60 +0.5 932,588 + PGA1_c09040 0.60 +0.4 932,596 - PGA1_c09040 0.61 +0.9 932,708 + PGA1_c09040 0.72 +2.0 932,722 + PGA1_c09040 0.73 +1.0 932,757 + PGA1_c09040 0.77 +0.4 932,760 - PGA1_c09040 0.77 +0.4 932,786 - PGA1_c09040 0.80 -0.4 932,789 + PGA1_c09040 0.80 +1.2 932,800 + PGA1_c09040 0.81 +0.8 932,849 + PGA1_c09040 0.86 +1.1 932,859 + PGA1_c09040 0.87 +1.0 932,859 + PGA1_c09040 0.87 +0.7 932,862 - PGA1_c09040 0.88 +0.9 932,981 - -0.5 932,981 - +1.3 932,981 - -0.2 933,114 + PGA1_c09050 0.26 +1.2 933,146 + PGA1_c09050 0.31 +1.4 933,154 - PGA1_c09050 0.33 +0.4 933,184 - PGA1_c09050 0.38 -0.2 933,186 + PGA1_c09050 0.38 +1.1 933,186 + PGA1_c09050 0.38 +0.3 933,213 - PGA1_c09050 0.43 +2.2 933,275 + PGA1_c09050 0.53 +1.5 933,283 - PGA1_c09050 0.54 +1.4 933,283 - PGA1_c09050 0.54 +0.1 933,312 - PGA1_c09050 0.59 -0.3 933,314 + PGA1_c09050 0.60 +1.6 933,314 + PGA1_c09050 0.60 +1.3 933,322 - PGA1_c09050 0.61 +1.5 933,322 - PGA1_c09050 0.61 +0.0 933,484 + PGA1_c09050 0.88 -1.5 933,488 + PGA1_c09050 0.89 -0.2 933,518 + +0.8 933,575 + +0.1 933,580 + +0.6 933,581 - +1.1 933,583 - +0.7 933,583 - +1.2 933,583 - +1.2 933,583 - -1.5 933,603 - +0.7 933,689 + PGA1_c09070 0.15 +0.6 933,702 - PGA1_c09070 0.16 +0.8 933,731 + PGA1_c09070 0.20 +0.6 933,731 + PGA1_c09070 0.20 +1.0 933,739 - PGA1_c09070 0.21 +0.6 933,767 + PGA1_c09070 0.25 +1.1 933,862 + PGA1_c09070 0.37 -0.2 933,865 - PGA1_c09070 0.38 +1.0 933,878 + PGA1_c09070 0.39 -0.5 933,881 - PGA1_c09070 0.40 +0.9 933,901 - PGA1_c09070 0.42 +0.5 933,948 + PGA1_c09070 0.48 +1.4 933,972 + PGA1_c09070 0.51 +0.8 933,978 - PGA1_c09070 0.52 +1.0 933,980 - PGA1_c09070 0.52 +1.3 934,004 + PGA1_c09070 0.56 +1.1 934,004 + PGA1_c09070 0.56 -0.2 934,004 + PGA1_c09070 0.56 +0.4 934,004 + PGA1_c09070 0.56 +1.3 934,004 + PGA1_c09070 0.56 +0.6 934,004 + PGA1_c09070 0.56 +0.2 934,004 + PGA1_c09070 0.56 +0.5 934,004 + PGA1_c09070 0.56 +0.3 934,012 - PGA1_c09070 0.57 +1.0 934,012 - PGA1_c09070 0.57 +1.0 934,012 - PGA1_c09070 0.57 +0.6 934,012 - PGA1_c09070 0.57 +0.2 934,012 - PGA1_c09070 0.57 -0.3 934,012 - PGA1_c09070 0.57 +0.3 934,012 - PGA1_c09070 0.57 +1.0 934,012 - PGA1_c09070 0.57 -0.2 934,012 - PGA1_c09070 0.57 +1.7 934,076 + PGA1_c09070 0.65 +1.1 934,084 - PGA1_c09070 0.66 +1.0 934,103 - PGA1_c09070 0.68 +0.3 934,103 - PGA1_c09070 0.68 +1.0 934,108 + PGA1_c09070 0.69 +1.6 934,142 + PGA1_c09070 0.74 +0.7 934,142 + PGA1_c09070 0.74 +0.9 934,150 - PGA1_c09070 0.75 +1.5 934,152 + PGA1_c09070 0.75 +1.4 934,152 + PGA1_c09070 0.75 +1.1 934,162 + PGA1_c09070 0.76 +1.3 934,275 + +0.5 934,275 - -0.1 934,306 - +0.1 934,311 + +0.8 934,311 + +0.9 934,311 + +0.8 934,319 - +0.9 934,339 - +0.3 934,354 - -2.3 934,364 + +0.9 934,472 - PGA1_c09090 0.63 +0.7 934,513 - PGA1_c09090 0.83 +1.0 934,529 - -0.3 934,539 + +1.6
Or see this region's nucleotide sequence