Experiment: m.b. Phosphomycin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c22090 and PGA1_c22100 are separated by 28 nucleotides PGA1_c22100 and PGA1_c22110 are separated by 456 nucleotides PGA1_c22110 and PGA1_c22120 are separated by 14 nucleotides PGA1_c22120 and PGA1_c22130 overlap by 4 nucleotides
PGA1_c22090: PGA1_c22090 - nitroreductase-like protein, at 2,289,724 to 2,290,395
_c22090
PGA1_c22100: PGA1_c22100 - D-tyrosyl-tRNA(Tyr) deacylase Dtd, at 2,290,424 to 2,290,870
_c22100
PGA1_c22110: PGA1_c22110 - putative glutathione-binding protein GsiB, at 2,291,327 to 2,292,913
_c22110
PGA1_c22120: PGA1_c22120 - glutathione transport system permease protein GsiC, at 2,292,928 to 2,293,884
_c22120
PGA1_c22130: PGA1_c22130 - putative glutathione transport system permease protein GsiD, at 2,293,881 to 2,294,696
_c22130
Position (kb)
2291
2292
2293 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2290.344 kb on + strand at 2290.344 kb on + strand at 2290.372 kb on - strand at 2290.393 kb on - strand at 2290.410 kb on + strand at 2290.439 kb on - strand at 2290.439 kb on - strand at 2290.521 kb on - strand, within PGA1_c22100 at 2290.526 kb on - strand, within PGA1_c22100 at 2290.558 kb on - strand, within PGA1_c22100 at 2290.716 kb on + strand, within PGA1_c22100 at 2290.716 kb on + strand, within PGA1_c22100 at 2290.716 kb on + strand, within PGA1_c22100 at 2290.716 kb on + strand, within PGA1_c22100 at 2290.721 kb on + strand, within PGA1_c22100 at 2290.724 kb on - strand, within PGA1_c22100 at 2290.724 kb on - strand, within PGA1_c22100 at 2290.724 kb on - strand, within PGA1_c22100 at 2290.729 kb on - strand, within PGA1_c22100 at 2290.740 kb on + strand, within PGA1_c22100 at 2290.767 kb on + strand, within PGA1_c22100 at 2290.817 kb on + strand, within PGA1_c22100 at 2290.865 kb on - strand at 2290.905 kb on + strand at 2290.913 kb on - strand at 2290.913 kb on - strand at 2291.002 kb on + strand at 2291.043 kb on + strand at 2291.050 kb on + strand at 2291.051 kb on - strand at 2291.139 kb on + strand at 2291.199 kb on - strand at 2291.241 kb on - strand at 2291.260 kb on - strand at 2291.301 kb on - strand at 2291.314 kb on - strand at 2291.314 kb on - strand at 2291.413 kb on + strand at 2291.429 kb on + strand at 2291.437 kb on - strand at 2291.437 kb on - strand at 2291.437 kb on - strand at 2291.464 kb on - strand at 2291.582 kb on + strand, within PGA1_c22110 at 2291.619 kb on - strand, within PGA1_c22110 at 2291.665 kb on + strand, within PGA1_c22110 at 2291.665 kb on + strand, within PGA1_c22110 at 2291.716 kb on + strand, within PGA1_c22110 at 2291.740 kb on - strand, within PGA1_c22110 at 2291.809 kb on + strand, within PGA1_c22110 at 2291.815 kb on + strand, within PGA1_c22110 at 2291.835 kb on + strand, within PGA1_c22110 at 2292.021 kb on - strand, within PGA1_c22110 at 2292.108 kb on - strand, within PGA1_c22110 at 2292.130 kb on + strand, within PGA1_c22110 at 2292.148 kb on - strand, within PGA1_c22110 at 2292.378 kb on + strand, within PGA1_c22110 at 2292.481 kb on - strand, within PGA1_c22110 at 2292.491 kb on - strand, within PGA1_c22110 at 2292.569 kb on - strand, within PGA1_c22110 at 2292.603 kb on + strand, within PGA1_c22110 at 2292.623 kb on + strand, within PGA1_c22110 at 2292.623 kb on + strand, within PGA1_c22110 at 2292.667 kb on - strand, within PGA1_c22110 at 2292.730 kb on - strand, within PGA1_c22110 at 2292.756 kb on + strand at 2292.788 kb on + strand at 2292.796 kb on - strand at 2292.839 kb on + strand at 2292.839 kb on + strand at 2292.844 kb on + strand at 2292.918 kb on + strand at 2292.918 kb on + strand at 2292.926 kb on - strand at 2292.954 kb on - strand at 2292.957 kb on + strand at 2292.971 kb on + strand at 2292.979 kb on - strand at 2293.026 kb on + strand, within PGA1_c22120 at 2293.042 kb on - strand, within PGA1_c22120 at 2293.067 kb on + strand, within PGA1_c22120 at 2293.067 kb on + strand, within PGA1_c22120 at 2293.075 kb on - strand, within PGA1_c22120 at 2293.075 kb on - strand, within PGA1_c22120 at 2293.186 kb on + strand, within PGA1_c22120 at 2293.186 kb on + strand, within PGA1_c22120 at 2293.186 kb on + strand, within PGA1_c22120 at 2293.194 kb on - strand, within PGA1_c22120 at 2293.287 kb on + strand, within PGA1_c22120 at 2293.329 kb on + strand, within PGA1_c22120 at 2293.336 kb on + strand, within PGA1_c22120 at 2293.336 kb on + strand, within PGA1_c22120 at 2293.350 kb on - strand, within PGA1_c22120 at 2293.392 kb on - strand, within PGA1_c22120 at 2293.422 kb on - strand, within PGA1_c22120 at 2293.503 kb on - strand, within PGA1_c22120 at 2293.503 kb on - strand, within PGA1_c22120 at 2293.503 kb on - strand, within PGA1_c22120 at 2293.548 kb on + strand, within PGA1_c22120 at 2293.548 kb on + strand, within PGA1_c22120 at 2293.548 kb on + strand, within PGA1_c22120 at 2293.604 kb on + strand, within PGA1_c22120 at 2293.604 kb on + strand, within PGA1_c22120 at 2293.612 kb on - strand, within PGA1_c22120 at 2293.632 kb on + strand, within PGA1_c22120 at 2293.632 kb on + strand, within PGA1_c22120 at 2293.635 kb on - strand, within PGA1_c22120 at 2293.682 kb on + strand, within PGA1_c22120 at 2293.690 kb on - strand, within PGA1_c22120 at 2293.690 kb on - strand, within PGA1_c22120 at 2293.694 kb on + strand, within PGA1_c22120 at 2293.737 kb on + strand, within PGA1_c22120 at 2293.740 kb on + strand, within PGA1_c22120 at 2293.788 kb on - strand, within PGA1_c22120 at 2293.788 kb on - strand, within PGA1_c22120 at 2293.788 kb on - strand, within PGA1_c22120 at 2293.793 kb on - strand at 2293.809 kb on + strand at 2293.864 kb on + strand at 2293.864 kb on + strand at 2293.872 kb on - strand at 2293.872 kb on - strand at 2293.872 kb on - strand at 2293.872 kb on - strand at 2293.898 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Phosphomycin 2 mg/ml remove 2,290,344 + +0.0 2,290,344 + +0.8 2,290,372 - +0.5 2,290,393 - -0.0 2,290,410 + +0.3 2,290,439 - -0.8 2,290,439 - +0.7 2,290,521 - PGA1_c22100 0.22 -0.6 2,290,526 - PGA1_c22100 0.23 +0.3 2,290,558 - PGA1_c22100 0.30 -0.4 2,290,716 + PGA1_c22100 0.65 +1.9 2,290,716 + PGA1_c22100 0.65 +0.1 2,290,716 + PGA1_c22100 0.65 -0.3 2,290,716 + PGA1_c22100 0.65 -1.1 2,290,721 + PGA1_c22100 0.66 +0.7 2,290,724 - PGA1_c22100 0.67 -0.9 2,290,724 - PGA1_c22100 0.67 -0.4 2,290,724 - PGA1_c22100 0.67 -1.0 2,290,729 - PGA1_c22100 0.68 +1.1 2,290,740 + PGA1_c22100 0.71 +0.3 2,290,767 + PGA1_c22100 0.77 +0.6 2,290,817 + PGA1_c22100 0.88 +0.4 2,290,865 - +0.6 2,290,905 + -0.5 2,290,913 - +0.1 2,290,913 - -0.8 2,291,002 + -0.3 2,291,043 + +0.7 2,291,050 + +1.0 2,291,051 - -0.2 2,291,139 + +0.3 2,291,199 - +1.6 2,291,241 - -0.3 2,291,260 - -0.1 2,291,301 - +0.7 2,291,314 - -0.6 2,291,314 - +0.3 2,291,413 + -0.2 2,291,429 + -1.3 2,291,437 - +0.3 2,291,437 - -2.7 2,291,437 - +0.9 2,291,464 - +1.8 2,291,582 + PGA1_c22110 0.16 -0.5 2,291,619 - PGA1_c22110 0.18 -1.5 2,291,665 + PGA1_c22110 0.21 -0.9 2,291,665 + PGA1_c22110 0.21 -1.2 2,291,716 + PGA1_c22110 0.25 -1.4 2,291,740 - PGA1_c22110 0.26 -0.5 2,291,809 + PGA1_c22110 0.30 -2.0 2,291,815 + PGA1_c22110 0.31 +0.2 2,291,835 + PGA1_c22110 0.32 +0.8 2,292,021 - PGA1_c22110 0.44 +0.2 2,292,108 - PGA1_c22110 0.49 -0.9 2,292,130 + PGA1_c22110 0.51 -0.8 2,292,148 - PGA1_c22110 0.52 -0.5 2,292,378 + PGA1_c22110 0.66 -0.3 2,292,481 - PGA1_c22110 0.73 -1.7 2,292,491 - PGA1_c22110 0.73 -0.8 2,292,569 - PGA1_c22110 0.78 +0.6 2,292,603 + PGA1_c22110 0.80 +1.0 2,292,623 + PGA1_c22110 0.82 -1.1 2,292,623 + PGA1_c22110 0.82 -0.4 2,292,667 - PGA1_c22110 0.84 -0.0 2,292,730 - PGA1_c22110 0.88 -1.1 2,292,756 + -1.0 2,292,788 + -1.0 2,292,796 - -1.2 2,292,839 + +0.5 2,292,839 + +0.6 2,292,844 + +1.6 2,292,918 + -1.2 2,292,918 + +0.2 2,292,926 - -0.9 2,292,954 - +0.5 2,292,957 + -1.2 2,292,971 + -0.3 2,292,979 - -0.8 2,293,026 + PGA1_c22120 0.10 +2.0 2,293,042 - PGA1_c22120 0.12 +0.5 2,293,067 + PGA1_c22120 0.15 -0.6 2,293,067 + PGA1_c22120 0.15 +0.7 2,293,075 - PGA1_c22120 0.15 +0.7 2,293,075 - PGA1_c22120 0.15 -1.1 2,293,186 + PGA1_c22120 0.27 +0.5 2,293,186 + PGA1_c22120 0.27 -1.8 2,293,186 + PGA1_c22120 0.27 -1.9 2,293,194 - PGA1_c22120 0.28 -0.7 2,293,287 + PGA1_c22120 0.38 -0.6 2,293,329 + PGA1_c22120 0.42 -0.1 2,293,336 + PGA1_c22120 0.43 +0.5 2,293,336 + PGA1_c22120 0.43 +0.1 2,293,350 - PGA1_c22120 0.44 -0.2 2,293,392 - PGA1_c22120 0.48 -0.2 2,293,422 - PGA1_c22120 0.52 -0.5 2,293,503 - PGA1_c22120 0.60 +1.1 2,293,503 - PGA1_c22120 0.60 -0.1 2,293,503 - PGA1_c22120 0.60 -0.3 2,293,548 + PGA1_c22120 0.65 -2.8 2,293,548 + PGA1_c22120 0.65 +0.1 2,293,548 + PGA1_c22120 0.65 -1.4 2,293,604 + PGA1_c22120 0.71 -0.8 2,293,604 + PGA1_c22120 0.71 -0.9 2,293,612 - PGA1_c22120 0.71 +0.3 2,293,632 + PGA1_c22120 0.74 -0.6 2,293,632 + PGA1_c22120 0.74 -0.5 2,293,635 - PGA1_c22120 0.74 -0.2 2,293,682 + PGA1_c22120 0.79 +0.7 2,293,690 - PGA1_c22120 0.80 -2.0 2,293,690 - PGA1_c22120 0.80 -1.1 2,293,694 + PGA1_c22120 0.80 -0.2 2,293,737 + PGA1_c22120 0.85 -0.2 2,293,740 + PGA1_c22120 0.85 -1.1 2,293,788 - PGA1_c22120 0.90 -0.9 2,293,788 - PGA1_c22120 0.90 +0.2 2,293,788 - PGA1_c22120 0.90 +0.9 2,293,793 - -0.2 2,293,809 + -0.9 2,293,864 + -0.5 2,293,864 + -0.5 2,293,872 - +0.2 2,293,872 - +0.1 2,293,872 - +0.9 2,293,872 - +1.0 2,293,898 - +0.2
Or see this region's nucleotide sequence