Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Xcc-8004.3748.1 and Xcc-8004.3749.1 are separated by 149 nucleotides Xcc-8004.3749.1 and Xcc-8004.3750.1 are separated by 82 nucleotides Xcc-8004.3750.1 and Xcc-8004.3751.1 are separated by 11 nucleotides Xcc-8004.3751.1 and Xcc-8004.3752.1 overlap by 4 nucleotides
Xcc-8004.3748.1: Xcc-8004.3748.1 - (AJ250023) putative polyketide synthase, at 3,620,451 to 3,621,185
Xcc-8004.3748.1
Xcc-8004.3749.1: Xcc-8004.3749.1 - hypothetical protein, at 3,621,335 to 3,621,961
Xcc-8004.3749.1
Xcc-8004.3750.1: Xcc-8004.3750.1 - Aspartate aminotransferase (EC 2.6.1.1), at 3,622,044 to 3,623,168
Xcc-8004.3750.1
Xcc-8004.3751.1: Xcc-8004.3751.1 - Short-chain dehydrogenase/reductase SDR, at 3,623,180 to 3,623,971
Xcc-8004.3751.1
Xcc-8004.3752.1: Xcc-8004.3752.1 - Isopenicillin N epimerase (EC 5.1.1.17), at 3,623,968 to 3,625,224
Xcc-8004.3752.1
Position (kb)
3622
3623
3624 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3621.199 kb on + strand at 3621.199 kb on + strand at 3621.199 kb on + strand at 3621.199 kb on + strand at 3621.199 kb on + strand at 3621.200 kb on - strand at 3621.200 kb on - strand at 3621.200 kb on - strand at 3621.204 kb on + strand at 3621.204 kb on + strand at 3621.204 kb on + strand at 3621.205 kb on - strand at 3621.251 kb on + strand at 3621.251 kb on + strand at 3621.251 kb on + strand at 3621.251 kb on + strand at 3621.251 kb on + strand at 3621.251 kb on + strand at 3621.252 kb on - strand at 3621.252 kb on - strand at 3621.252 kb on - strand at 3621.252 kb on - strand at 3621.252 kb on - strand at 3621.252 kb on - strand at 3621.294 kb on + strand at 3621.424 kb on + strand, within Xcc-8004.3749.1 at 3621.424 kb on + strand, within Xcc-8004.3749.1 at 3621.424 kb on + strand, within Xcc-8004.3749.1 at 3621.424 kb on + strand, within Xcc-8004.3749.1 at 3621.424 kb on + strand, within Xcc-8004.3749.1 at 3621.425 kb on - strand, within Xcc-8004.3749.1 at 3621.425 kb on - strand, within Xcc-8004.3749.1 at 3621.425 kb on - strand, within Xcc-8004.3749.1 at 3621.425 kb on - strand, within Xcc-8004.3749.1 at 3621.425 kb on - strand, within Xcc-8004.3749.1 at 3621.425 kb on - strand, within Xcc-8004.3749.1 at 3621.533 kb on + strand, within Xcc-8004.3749.1 at 3621.692 kb on + strand, within Xcc-8004.3749.1 at 3621.693 kb on - strand, within Xcc-8004.3749.1 at 3621.714 kb on + strand, within Xcc-8004.3749.1 at 3621.977 kb on + strand at 3621.978 kb on - strand at 3621.978 kb on - strand at 3622.147 kb on + strand at 3622.148 kb on - strand at 3622.178 kb on - strand, within Xcc-8004.3750.1 at 3622.375 kb on - strand, within Xcc-8004.3750.1 at 3622.438 kb on - strand, within Xcc-8004.3750.1 at 3622.597 kb on + strand, within Xcc-8004.3750.1 at 3622.598 kb on - strand, within Xcc-8004.3750.1 at 3622.618 kb on + strand, within Xcc-8004.3750.1 at 3622.619 kb on - strand, within Xcc-8004.3750.1 at 3622.901 kb on - strand, within Xcc-8004.3750.1 at 3622.901 kb on - strand, within Xcc-8004.3750.1 at 3623.009 kb on - strand, within Xcc-8004.3750.1 at 3623.169 kb on - strand at 3623.169 kb on - strand at 3623.169 kb on - strand at 3623.169 kb on - strand at 3623.169 kb on - strand at 3623.172 kb on + strand at 3623.269 kb on - strand, within Xcc-8004.3751.1 at 3623.369 kb on - strand, within Xcc-8004.3751.1 at 3623.408 kb on + strand, within Xcc-8004.3751.1 at 3623.409 kb on - strand, within Xcc-8004.3751.1 at 3623.409 kb on - strand, within Xcc-8004.3751.1 at 3623.656 kb on - strand, within Xcc-8004.3751.1 at 3623.775 kb on - strand, within Xcc-8004.3751.1 at 3623.775 kb on - strand, within Xcc-8004.3751.1 at 3623.775 kb on - strand, within Xcc-8004.3751.1 at 3623.775 kb on - strand, within Xcc-8004.3751.1 at 3624.077 kb on + strand at 3624.078 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 3,621,199 + -0.7 3,621,199 + -0.2 3,621,199 + +0.9 3,621,199 + +0.1 3,621,199 + -1.1 3,621,200 - -0.3 3,621,200 - -1.0 3,621,200 - -0.6 3,621,204 + +1.2 3,621,204 + -0.4 3,621,204 + -1.0 3,621,205 - +0.2 3,621,251 + +0.1 3,621,251 + +0.9 3,621,251 + -1.5 3,621,251 + -0.3 3,621,251 + -1.2 3,621,251 + +0.4 3,621,252 - +1.3 3,621,252 - +0.7 3,621,252 - +1.1 3,621,252 - -0.4 3,621,252 - -0.8 3,621,252 - +0.4 3,621,294 + -3.0 3,621,424 + Xcc-8004.3749.1 0.14 -1.0 3,621,424 + Xcc-8004.3749.1 0.14 +2.1 3,621,424 + Xcc-8004.3749.1 0.14 -0.5 3,621,424 + Xcc-8004.3749.1 0.14 -1.3 3,621,424 + Xcc-8004.3749.1 0.14 -1.6 3,621,425 - Xcc-8004.3749.1 0.14 +0.8 3,621,425 - Xcc-8004.3749.1 0.14 -1.5 3,621,425 - Xcc-8004.3749.1 0.14 +1.3 3,621,425 - Xcc-8004.3749.1 0.14 -0.9 3,621,425 - Xcc-8004.3749.1 0.14 +0.6 3,621,425 - Xcc-8004.3749.1 0.14 -3.0 3,621,533 + Xcc-8004.3749.1 0.32 -0.0 3,621,692 + Xcc-8004.3749.1 0.57 -0.3 3,621,693 - Xcc-8004.3749.1 0.57 -0.4 3,621,714 + Xcc-8004.3749.1 0.60 +0.8 3,621,977 + +0.7 3,621,978 - -1.3 3,621,978 - +2.5 3,622,147 + +0.3 3,622,148 - +0.1 3,622,178 - Xcc-8004.3750.1 0.12 -1.3 3,622,375 - Xcc-8004.3750.1 0.29 +0.3 3,622,438 - Xcc-8004.3750.1 0.35 -2.1 3,622,597 + Xcc-8004.3750.1 0.49 +1.8 3,622,598 - Xcc-8004.3750.1 0.49 -0.9 3,622,618 + Xcc-8004.3750.1 0.51 -0.1 3,622,619 - Xcc-8004.3750.1 0.51 +0.6 3,622,901 - Xcc-8004.3750.1 0.76 -0.3 3,622,901 - Xcc-8004.3750.1 0.76 -1.3 3,623,009 - Xcc-8004.3750.1 0.86 -0.9 3,623,169 - -0.5 3,623,169 - -1.8 3,623,169 - -0.4 3,623,169 - -0.8 3,623,169 - -2.3 3,623,172 + +0.8 3,623,269 - Xcc-8004.3751.1 0.11 +0.0 3,623,369 - Xcc-8004.3751.1 0.24 -0.2 3,623,408 + Xcc-8004.3751.1 0.29 +1.5 3,623,409 - Xcc-8004.3751.1 0.29 -3.3 3,623,409 - Xcc-8004.3751.1 0.29 -1.3 3,623,656 - Xcc-8004.3751.1 0.60 -0.7 3,623,775 - Xcc-8004.3751.1 0.75 -2.1 3,623,775 - Xcc-8004.3751.1 0.75 +0.2 3,623,775 - Xcc-8004.3751.1 0.75 +0.5 3,623,775 - Xcc-8004.3751.1 0.75 +0.3 3,624,077 + -1.0 3,624,078 - -0.1
Or see this region's nucleotide sequence