Experiment: m.b. Phosphomycin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c09050 and PGA1_c09070 are separated by 23 nucleotides PGA1_c09070 and PGA1_c09090 are separated by 3 nucleotides PGA1_c09090 and PGA1_c09100 overlap by 32 nucleotides PGA1_c09100 and PGA1_c09110 overlap by 4 nucleotides
PGA1_c09050: PGA1_c09050 - thiamine-phosphate pyrophosphorylase ThiE, at 932,960 to 933,553
_c09050
PGA1_c09070: PGA1_c09070 - thiazole biosynthesis protein ThiG, at 933,577 to 934,344
_c09070
PGA1_c09090: PGA1_c09090 - thiamine biosynthesis protein ThiS, at 934,348 to 934,545
_c09090
PGA1_c09100: PGA1_c09100 - putative thiamine biosynthesis oxidoreductase ThiO, at 934,514 to 935,488
_c09100
PGA1_c09110: PGA1_c09110 - thiamine biosynthesis protein ThiC, at 935,485 to 937,284
_c09110
Position (kb)
934
935 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 933.484 kb on + strand, within PGA1_c09050 at 933.488 kb on + strand, within PGA1_c09050 at 933.518 kb on + strand at 933.575 kb on + strand at 933.580 kb on + strand at 933.581 kb on - strand at 933.583 kb on - strand at 933.583 kb on - strand at 933.583 kb on - strand at 933.583 kb on - strand at 933.603 kb on - strand at 933.689 kb on + strand, within PGA1_c09070 at 933.702 kb on - strand, within PGA1_c09070 at 933.731 kb on + strand, within PGA1_c09070 at 933.731 kb on + strand, within PGA1_c09070 at 933.739 kb on - strand, within PGA1_c09070 at 933.767 kb on + strand, within PGA1_c09070 at 933.862 kb on + strand, within PGA1_c09070 at 933.865 kb on - strand, within PGA1_c09070 at 933.878 kb on + strand, within PGA1_c09070 at 933.881 kb on - strand, within PGA1_c09070 at 933.901 kb on - strand, within PGA1_c09070 at 933.948 kb on + strand, within PGA1_c09070 at 933.972 kb on + strand, within PGA1_c09070 at 933.978 kb on - strand, within PGA1_c09070 at 933.980 kb on - strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.076 kb on + strand, within PGA1_c09070 at 934.084 kb on - strand, within PGA1_c09070 at 934.103 kb on - strand, within PGA1_c09070 at 934.103 kb on - strand, within PGA1_c09070 at 934.108 kb on + strand, within PGA1_c09070 at 934.142 kb on + strand, within PGA1_c09070 at 934.142 kb on + strand, within PGA1_c09070 at 934.150 kb on - strand, within PGA1_c09070 at 934.152 kb on + strand, within PGA1_c09070 at 934.152 kb on + strand, within PGA1_c09070 at 934.162 kb on + strand, within PGA1_c09070 at 934.275 kb on + strand at 934.275 kb on - strand at 934.306 kb on - strand at 934.311 kb on + strand at 934.311 kb on + strand at 934.311 kb on + strand at 934.319 kb on - strand at 934.339 kb on - strand at 934.354 kb on - strand at 934.364 kb on + strand at 934.472 kb on - strand, within PGA1_c09090 at 934.513 kb on - strand, within PGA1_c09090 at 934.529 kb on - strand at 934.539 kb on + strand at 934.568 kb on - strand at 934.624 kb on - strand, within PGA1_c09100 at 934.636 kb on - strand, within PGA1_c09100 at 934.659 kb on - strand, within PGA1_c09100 at 934.659 kb on - strand, within PGA1_c09100 at 934.685 kb on + strand, within PGA1_c09100 at 934.685 kb on + strand, within PGA1_c09100 at 934.693 kb on - strand, within PGA1_c09100 at 934.693 kb on - strand, within PGA1_c09100 at 934.693 kb on - strand, within PGA1_c09100 at 934.710 kb on + strand, within PGA1_c09100 at 934.719 kb on - strand, within PGA1_c09100 at 934.846 kb on + strand, within PGA1_c09100 at 934.854 kb on - strand, within PGA1_c09100 at 934.854 kb on - strand, within PGA1_c09100 at 934.903 kb on + strand, within PGA1_c09100 at 934.943 kb on + strand, within PGA1_c09100 at 935.068 kb on - strand, within PGA1_c09100 at 935.081 kb on + strand, within PGA1_c09100 at 935.089 kb on - strand, within PGA1_c09100 at 935.139 kb on + strand, within PGA1_c09100 at 935.147 kb on - strand, within PGA1_c09100 at 935.147 kb on - strand, within PGA1_c09100 at 935.172 kb on + strand, within PGA1_c09100 at 935.177 kb on + strand, within PGA1_c09100 at 935.217 kb on - strand, within PGA1_c09100 at 935.228 kb on - strand, within PGA1_c09100 at 935.276 kb on - strand, within PGA1_c09100 at 935.351 kb on + strand, within PGA1_c09100 at 935.429 kb on - strand at 935.495 kb on + strand at 935.507 kb on + strand at 935.515 kb on - strand at 935.515 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Phosphomycin 1 mg/ml remove 933,484 + PGA1_c09050 0.88 +3.0 933,488 + PGA1_c09050 0.89 +2.7 933,518 + +2.8 933,575 + +1.6 933,580 + +2.7 933,581 - -0.7 933,583 - +0.1 933,583 - +2.1 933,583 - -1.6 933,583 - +1.2 933,603 - +2.8 933,689 + PGA1_c09070 0.15 +2.8 933,702 - PGA1_c09070 0.16 +3.5 933,731 + PGA1_c09070 0.20 +3.4 933,731 + PGA1_c09070 0.20 +2.9 933,739 - PGA1_c09070 0.21 +3.0 933,767 + PGA1_c09070 0.25 +3.2 933,862 + PGA1_c09070 0.37 +1.3 933,865 - PGA1_c09070 0.38 +2.8 933,878 + PGA1_c09070 0.39 +3.0 933,881 - PGA1_c09070 0.40 +3.6 933,901 - PGA1_c09070 0.42 +3.1 933,948 + PGA1_c09070 0.48 +3.1 933,972 + PGA1_c09070 0.51 +3.3 933,978 - PGA1_c09070 0.52 +3.9 933,980 - PGA1_c09070 0.52 +3.7 934,004 + PGA1_c09070 0.56 +3.4 934,004 + PGA1_c09070 0.56 +3.7 934,004 + PGA1_c09070 0.56 +2.9 934,004 + PGA1_c09070 0.56 +3.6 934,004 + PGA1_c09070 0.56 +3.1 934,004 + PGA1_c09070 0.56 +3.4 934,004 + PGA1_c09070 0.56 +2.8 934,004 + PGA1_c09070 0.56 +3.3 934,012 - PGA1_c09070 0.57 +2.2 934,012 - PGA1_c09070 0.57 +3.2 934,012 - PGA1_c09070 0.57 +3.2 934,012 - PGA1_c09070 0.57 +2.6 934,012 - PGA1_c09070 0.57 +3.4 934,012 - PGA1_c09070 0.57 +3.2 934,012 - PGA1_c09070 0.57 +3.4 934,012 - PGA1_c09070 0.57 +3.5 934,012 - PGA1_c09070 0.57 +2.5 934,076 + PGA1_c09070 0.65 +3.2 934,084 - PGA1_c09070 0.66 +3.3 934,103 - PGA1_c09070 0.68 +1.6 934,103 - PGA1_c09070 0.68 +4.0 934,108 + PGA1_c09070 0.69 +3.5 934,142 + PGA1_c09070 0.74 +3.5 934,142 + PGA1_c09070 0.74 +3.7 934,150 - PGA1_c09070 0.75 +3.0 934,152 + PGA1_c09070 0.75 +3.8 934,152 + PGA1_c09070 0.75 +3.6 934,162 + PGA1_c09070 0.76 +3.1 934,275 + +3.5 934,275 - +3.5 934,306 - +2.5 934,311 + +3.3 934,311 + +3.4 934,311 + +2.4 934,319 - +3.4 934,339 - +3.9 934,354 - -0.9 934,364 + +3.2 934,472 - PGA1_c09090 0.63 +3.4 934,513 - PGA1_c09090 0.83 +3.6 934,529 - +2.7 934,539 + +4.5 934,568 - -0.1 934,624 - PGA1_c09100 0.11 +2.9 934,636 - PGA1_c09100 0.13 +3.4 934,659 - PGA1_c09100 0.15 +3.4 934,659 - PGA1_c09100 0.15 +4.1 934,685 + PGA1_c09100 0.18 +3.4 934,685 + PGA1_c09100 0.18 +3.8 934,693 - PGA1_c09100 0.18 +2.6 934,693 - PGA1_c09100 0.18 +3.4 934,693 - PGA1_c09100 0.18 +2.1 934,710 + PGA1_c09100 0.20 +3.6 934,719 - PGA1_c09100 0.21 +1.7 934,846 + PGA1_c09100 0.34 +3.8 934,854 - PGA1_c09100 0.35 +3.2 934,854 - PGA1_c09100 0.35 +3.9 934,903 + PGA1_c09100 0.40 +3.5 934,943 + PGA1_c09100 0.44 +4.2 935,068 - PGA1_c09100 0.57 +2.5 935,081 + PGA1_c09100 0.58 +3.6 935,089 - PGA1_c09100 0.59 +3.5 935,139 + PGA1_c09100 0.64 +3.2 935,147 - PGA1_c09100 0.65 +2.9 935,147 - PGA1_c09100 0.65 +3.0 935,172 + PGA1_c09100 0.67 +2.4 935,177 + PGA1_c09100 0.68 +3.7 935,217 - PGA1_c09100 0.72 +2.7 935,228 - PGA1_c09100 0.73 +2.7 935,276 - PGA1_c09100 0.78 +0.8 935,351 + PGA1_c09100 0.86 +3.7 935,429 - +3.1 935,495 + +3.1 935,507 + +2.5 935,515 - +4.0 935,515 - +3.4
Or see this region's nucleotide sequence