Experiment: A22 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27935 and BWI76_RS27940 overlap by 4 nucleotides BWI76_RS27940 and BWI76_RS27945 overlap by 17 nucleotides BWI76_RS27945 and BWI76_RS27950 overlap by 4 nucleotides BWI76_RS27950 and BWI76_RS27955 are separated by 243 nucleotides
BWI76_RS27935: BWI76_RS27935 - galactonate dehydratase, at 5,793,557 to 5,794,705
_RS27935
BWI76_RS27940: BWI76_RS27940 - 2-dehydro-3-deoxy-6-phosphogalactonate aldolase, at 5,794,702 to 5,795,319
_RS27940
BWI76_RS27945: BWI76_RS27945 - 2-oxo-3-deoxygalactonate kinase, at 5,795,303 to 5,796,178
_RS27945
BWI76_RS27950: BWI76_RS27950 - transcriptional regulator, at 5,796,175 to 5,796,864
_RS27950
BWI76_RS27955: BWI76_RS27955 - sugar-phosphatase, at 5,797,108 to 5,797,920
_RS27955
Position (kb)
5795
5796
5797 Strain fitness (log2 ratio)
-2
-1
0
1 at 5794.360 kb on - strand, within BWI76_RS27935 at 5794.487 kb on + strand, within BWI76_RS27935 at 5794.509 kb on + strand, within BWI76_RS27935 at 5794.529 kb on - strand, within BWI76_RS27935 at 5794.573 kb on - strand, within BWI76_RS27935 at 5794.587 kb on - strand, within BWI76_RS27935 at 5794.715 kb on - strand at 5794.914 kb on - strand at 5795.026 kb on - strand, within BWI76_RS27940 at 5795.105 kb on - strand, within BWI76_RS27940 at 5795.111 kb on + strand, within BWI76_RS27940 at 5795.276 kb on - strand at 5795.291 kb on - strand at 5795.330 kb on + strand at 5795.338 kb on - strand at 5795.409 kb on - strand, within BWI76_RS27945 at 5795.707 kb on - strand, within BWI76_RS27945 at 5795.707 kb on - strand, within BWI76_RS27945 at 5795.815 kb on + strand, within BWI76_RS27945 at 5796.152 kb on + strand at 5796.328 kb on - strand, within BWI76_RS27950 at 5796.356 kb on - strand, within BWI76_RS27950 at 5796.381 kb on + strand, within BWI76_RS27950 at 5796.791 kb on - strand, within BWI76_RS27950 at 5796.828 kb on + strand at 5796.860 kb on + strand at 5796.891 kb on - strand at 5797.154 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.1 mg/ml remove 5,794,360 - BWI76_RS27935 0.70 +0.0 5,794,487 + BWI76_RS27935 0.81 +0.1 5,794,509 + BWI76_RS27935 0.83 +0.2 5,794,529 - BWI76_RS27935 0.85 +1.2 5,794,573 - BWI76_RS27935 0.88 +0.4 5,794,587 - BWI76_RS27935 0.90 -0.3 5,794,715 - -0.2 5,794,914 - -0.3 5,795,026 - BWI76_RS27940 0.52 +0.3 5,795,105 - BWI76_RS27940 0.65 -1.1 5,795,111 + BWI76_RS27940 0.66 -0.2 5,795,276 - +0.3 5,795,291 - +0.3 5,795,330 + -1.0 5,795,338 - -1.1 5,795,409 - BWI76_RS27945 0.12 -0.2 5,795,707 - BWI76_RS27945 0.46 +0.0 5,795,707 - BWI76_RS27945 0.46 -2.1 5,795,815 + BWI76_RS27945 0.58 -1.0 5,796,152 + -0.4 5,796,328 - BWI76_RS27950 0.22 +0.1 5,796,356 - BWI76_RS27950 0.26 +0.1 5,796,381 + BWI76_RS27950 0.30 +0.6 5,796,791 - BWI76_RS27950 0.89 +0.0 5,796,828 + +0.4 5,796,860 + +0.1 5,796,891 - +0.3 5,797,154 + -0.4
Or see this region's nucleotide sequence