Experiment: A22 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27815 and BWI76_RS27820 are separated by 102 nucleotides BWI76_RS27820 and BWI76_RS27825 are separated by 26 nucleotides BWI76_RS27825 and BWI76_RS27830 are separated by 313 nucleotides BWI76_RS27830 and BWI76_RS27835 are separated by 70 nucleotides BWI76_RS27835 and BWI76_RS27840 are separated by 70 nucleotides
BWI76_RS27815: BWI76_RS27815 - acetolactate synthase catalytic subunit, at 5,770,640 to 5,772,328
_RS27815
BWI76_RS27820: BWI76_RS27820 - ilvB operon leader peptide IvbL, at 5,772,431 to 5,772,526
_RS27820
BWI76_RS27825: BWI76_RS27825 - hypothetical protein, at 5,772,553 to 5,772,795
_RS27825
BWI76_RS27830: BWI76_RS27830 - type I toxin-antitoxin system toxin TisB, at 5,773,109 to 5,773,198
_RS27830
BWI76_RS27835: BWI76_RS27835 - acyltransferase, at 5,773,269 to 5,773,715
_RS27835
BWI76_RS27840: BWI76_RS27840 - putative membrane protein, transport, at 5,773,786 to 5,774,619
_RS27840
Position (kb) 5773
5774 Strain fitness (log2 ratio) -1
0
1 at 5772.125 kb on - strand, within BWI76_RS27815 at 5772.248 kb on + strand at 5772.499 kb on + strand, within BWI76_RS27820 at 5772.501 kb on + strand, within BWI76_RS27820 at 5772.519 kb on + strand at 5772.519 kb on - strand at 5772.721 kb on + strand, within BWI76_RS27825 at 5772.729 kb on - strand, within BWI76_RS27825 at 5772.729 kb on - strand, within BWI76_RS27825 at 5772.795 kb on - strand at 5772.884 kb on - strand at 5773.054 kb on - strand at 5773.068 kb on - strand at 5773.130 kb on + strand, within BWI76_RS27830 at 5773.178 kb on + strand, within BWI76_RS27830 at 5773.178 kb on + strand, within BWI76_RS27830 at 5773.224 kb on - strand at 5773.305 kb on - strand at 5773.445 kb on - strand, within BWI76_RS27835 at 5773.488 kb on - strand, within BWI76_RS27835 at 5773.582 kb on - strand, within BWI76_RS27835 at 5773.677 kb on - strand at 5773.817 kb on + strand at 5773.898 kb on + strand, within BWI76_RS27840 at 5773.929 kb on + strand, within BWI76_RS27840 at 5773.951 kb on - strand, within BWI76_RS27840 at 5774.008 kb on - strand, within BWI76_RS27840 at 5774.029 kb on - strand, within BWI76_RS27840 at 5774.184 kb on - strand, within BWI76_RS27840 at 5774.195 kb on + strand, within BWI76_RS27840
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.1 mg/ml remove 5,772,125 - BWI76_RS27815 0.88 +0.2 5,772,248 + +0.3 5,772,499 + BWI76_RS27820 0.71 -0.9 5,772,501 + BWI76_RS27820 0.73 +0.4 5,772,519 + -0.1 5,772,519 - -0.0 5,772,721 + BWI76_RS27825 0.69 -0.8 5,772,729 - BWI76_RS27825 0.72 +0.5 5,772,729 - BWI76_RS27825 0.72 -0.5 5,772,795 - -0.2 5,772,884 - -0.8 5,773,054 - -0.4 5,773,068 - -0.1 5,773,130 + BWI76_RS27830 0.23 -0.2 5,773,178 + BWI76_RS27830 0.77 -0.7 5,773,178 + BWI76_RS27830 0.77 -1.1 5,773,224 - -0.3 5,773,305 - -0.8 5,773,445 - BWI76_RS27835 0.39 -0.6 5,773,488 - BWI76_RS27835 0.49 +1.6 5,773,582 - BWI76_RS27835 0.70 -0.4 5,773,677 - -0.4 5,773,817 + +0.2 5,773,898 + BWI76_RS27840 0.13 +0.4 5,773,929 + BWI76_RS27840 0.17 -0.6 5,773,951 - BWI76_RS27840 0.20 -0.1 5,774,008 - BWI76_RS27840 0.27 -0.2 5,774,029 - BWI76_RS27840 0.29 +0.2 5,774,184 - BWI76_RS27840 0.48 +0.2 5,774,195 + BWI76_RS27840 0.49 -0.5
Or see this region's nucleotide sequence