Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS27235

Experiment: A22 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS27230 and BWI76_RS27235 are separated by 9 nucleotidesBWI76_RS27235 and BWI76_RS27240 are separated by 3 nucleotidesBWI76_RS27240 and BWI76_RS27245 overlap by 4 nucleotides BWI76_RS27230: BWI76_RS27230 - 2,3-bisphosphoglycerate-independent phosphoglycerate mutase, at 5,650,762 to 5,652,306 _RS27230 BWI76_RS27235: BWI76_RS27235 - murein hydrolase activator EnvC, at 5,652,316 to 5,653,587 _RS27235 BWI76_RS27240: BWI76_RS27240 - hypothetical protein, at 5,653,591 to 5,654,523 _RS27240 BWI76_RS27245: BWI76_RS27245 - glycosyl transferase family protein, at 5,654,520 to 5,655,317 _RS27245 Position (kb) 5652 5653 5654Strain fitness (log2 ratio) -2 -1 0 1at 5651.362 kb on + strand, within BWI76_RS27230at 5651.400 kb on + strand, within BWI76_RS27230at 5651.502 kb on - strand, within BWI76_RS27230at 5651.664 kb on + strand, within BWI76_RS27230at 5651.672 kb on - strand, within BWI76_RS27230at 5651.966 kb on + strand, within BWI76_RS27230at 5652.030 kb on - strand, within BWI76_RS27230at 5652.104 kb on - strand, within BWI76_RS27230at 5652.135 kb on + strand, within BWI76_RS27230at 5652.135 kb on + strand, within BWI76_RS27230at 5652.143 kb on - strand, within BWI76_RS27230at 5652.301 kb on + strandat 5652.320 kb on + strandat 5652.395 kb on - strandat 5652.406 kb on - strandat 5652.467 kb on - strand, within BWI76_RS27235at 5652.528 kb on + strand, within BWI76_RS27235at 5652.528 kb on + strand, within BWI76_RS27235at 5652.536 kb on - strand, within BWI76_RS27235at 5652.540 kb on + strand, within BWI76_RS27235at 5652.540 kb on + strand, within BWI76_RS27235at 5652.546 kb on + strand, within BWI76_RS27235at 5652.782 kb on - strand, within BWI76_RS27235at 5652.842 kb on + strand, within BWI76_RS27235at 5652.929 kb on - strand, within BWI76_RS27235at 5653.100 kb on + strand, within BWI76_RS27235at 5653.122 kb on + strand, within BWI76_RS27235at 5653.148 kb on + strand, within BWI76_RS27235at 5653.204 kb on - strand, within BWI76_RS27235at 5653.266 kb on + strand, within BWI76_RS27235at 5653.272 kb on + strand, within BWI76_RS27235at 5653.323 kb on + strand, within BWI76_RS27235at 5653.454 kb on - strand, within BWI76_RS27235at 5653.464 kb on + strandat 5653.507 kb on + strandat 5653.562 kb on + strandat 5653.570 kb on - strandat 5653.643 kb on - strandat 5653.763 kb on - strand, within BWI76_RS27240at 5653.879 kb on + strand, within BWI76_RS27240at 5653.900 kb on - strand, within BWI76_RS27240at 5653.903 kb on - strand, within BWI76_RS27240at 5653.948 kb on - strand, within BWI76_RS27240at 5653.962 kb on + strand, within BWI76_RS27240at 5653.965 kb on - strand, within BWI76_RS27240at 5653.998 kb on - strand, within BWI76_RS27240at 5654.067 kb on + strand, within BWI76_RS27240at 5654.085 kb on + strand, within BWI76_RS27240at 5654.088 kb on - strand, within BWI76_RS27240at 5654.187 kb on + strand, within BWI76_RS27240at 5654.195 kb on - strand, within BWI76_RS27240at 5654.223 kb on + strand, within BWI76_RS27240at 5654.254 kb on + strand, within BWI76_RS27240at 5654.254 kb on + strand, within BWI76_RS27240at 5654.278 kb on + strand, within BWI76_RS27240at 5654.295 kb on - strand, within BWI76_RS27240at 5654.332 kb on - strand, within BWI76_RS27240at 5654.568 kb on + strandat 5654.571 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction A22 0.1 mg/ml
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5,651,362 + BWI76_RS27230 0.39 +0.1
5,651,400 + BWI76_RS27230 0.41 -0.5
5,651,502 - BWI76_RS27230 0.48 +1.3
5,651,664 + BWI76_RS27230 0.58 -0.4
5,651,672 - BWI76_RS27230 0.59 +0.0
5,651,966 + BWI76_RS27230 0.78 +0.1
5,652,030 - BWI76_RS27230 0.82 +0.3
5,652,104 - BWI76_RS27230 0.87 +0.2
5,652,135 + BWI76_RS27230 0.89 +1.1
5,652,135 + BWI76_RS27230 0.89 +0.0
5,652,143 - BWI76_RS27230 0.89 +0.4
5,652,301 + -1.6
5,652,320 + -2.6
5,652,395 - -1.6
5,652,406 - -2.5
5,652,467 - BWI76_RS27235 0.12 -1.9
5,652,528 + BWI76_RS27235 0.17 -1.5
5,652,528 + BWI76_RS27235 0.17 -2.0
5,652,536 - BWI76_RS27235 0.17 -1.2
5,652,540 + BWI76_RS27235 0.18 -1.7
5,652,540 + BWI76_RS27235 0.18 -0.7
5,652,546 + BWI76_RS27235 0.18 -2.3
5,652,782 - BWI76_RS27235 0.37 -2.4
5,652,842 + BWI76_RS27235 0.41 -1.5
5,652,929 - BWI76_RS27235 0.48 -2.0
5,653,100 + BWI76_RS27235 0.62 -1.2
5,653,122 + BWI76_RS27235 0.63 -2.7
5,653,148 + BWI76_RS27235 0.65 +0.0
5,653,204 - BWI76_RS27235 0.70 -2.3
5,653,266 + BWI76_RS27235 0.75 -2.4
5,653,272 + BWI76_RS27235 0.75 -2.2
5,653,323 + BWI76_RS27235 0.79 -2.0
5,653,454 - BWI76_RS27235 0.89 -2.2
5,653,464 + -1.3
5,653,507 + -1.8
5,653,562 + -0.8
5,653,570 - -1.6
5,653,643 - -1.0
5,653,763 - BWI76_RS27240 0.18 -0.6
5,653,879 + BWI76_RS27240 0.31 +0.1
5,653,900 - BWI76_RS27240 0.33 +0.3
5,653,903 - BWI76_RS27240 0.33 -1.0
5,653,948 - BWI76_RS27240 0.38 -0.6
5,653,962 + BWI76_RS27240 0.40 +1.4
5,653,965 - BWI76_RS27240 0.40 -0.6
5,653,998 - BWI76_RS27240 0.44 -1.2
5,654,067 + BWI76_RS27240 0.51 -0.7
5,654,085 + BWI76_RS27240 0.53 -0.2
5,654,088 - BWI76_RS27240 0.53 +1.2
5,654,187 + BWI76_RS27240 0.64 +0.3
5,654,195 - BWI76_RS27240 0.65 +0.2
5,654,223 + BWI76_RS27240 0.68 -0.1
5,654,254 + BWI76_RS27240 0.71 +0.1
5,654,254 + BWI76_RS27240 0.71 +0.4
5,654,278 + BWI76_RS27240 0.74 -0.6
5,654,295 - BWI76_RS27240 0.75 -0.8
5,654,332 - BWI76_RS27240 0.79 +1.3
5,654,568 + -0.3
5,654,571 - +0.2

Or see this region's nucleotide sequence