Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS26765

Experiment: A22 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS26760 and BWI76_RS26765 are separated by 154 nucleotidesBWI76_RS26765 and BWI76_RS26770 are separated by 436 nucleotides BWI76_RS26760: BWI76_RS26760 - C4-dicarboxylate transport protein DctA (from data), at 5,542,491 to 5,543,777 _RS26760 BWI76_RS26765: BWI76_RS26765 - phosphodiesterase, at 5,543,932 to 5,545,938 _RS26765 BWI76_RS26770: BWI76_RS26770 - cellulose biosynthesis protein BcsC, at 5,546,375 to 5,549,860 _RS26770 Position (kb) 5543 5544 5545 5546Strain fitness (log2 ratio) -2 -1 0 1 2 3at 5543.274 kb on + strandat 5543.321 kb on - strand, within BWI76_RS26760at 5543.402 kb on - strand, within BWI76_RS26760at 5543.446 kb on + strand, within BWI76_RS26760at 5543.513 kb on + strand, within BWI76_RS26760at 5543.516 kb on + strand, within BWI76_RS26760at 5543.554 kb on - strand, within BWI76_RS26760at 5543.616 kb on + strand, within BWI76_RS26760at 5543.648 kb on + strand, within BWI76_RS26760at 5543.672 kb on - strandat 5543.738 kb on - strandat 5543.753 kb on + strandat 5543.851 kb on - strandat 5544.009 kb on - strandat 5544.114 kb on + strandat 5544.122 kb on - strandat 5544.230 kb on - strand, within BWI76_RS26765at 5544.235 kb on - strand, within BWI76_RS26765at 5544.240 kb on - strand, within BWI76_RS26765at 5544.614 kb on - strand, within BWI76_RS26765at 5544.636 kb on - strand, within BWI76_RS26765at 5544.735 kb on + strand, within BWI76_RS26765at 5544.743 kb on - strand, within BWI76_RS26765at 5544.801 kb on + strand, within BWI76_RS26765at 5544.879 kb on - strand, within BWI76_RS26765at 5544.881 kb on - strand, within BWI76_RS26765at 5545.043 kb on + strand, within BWI76_RS26765at 5545.096 kb on - strand, within BWI76_RS26765at 5545.171 kb on + strand, within BWI76_RS26765at 5545.223 kb on - strand, within BWI76_RS26765at 5545.562 kb on - strand, within BWI76_RS26765at 5545.675 kb on + strand, within BWI76_RS26765at 5545.735 kb on - strand, within BWI76_RS26765at 5545.735 kb on - strand, within BWI76_RS26765at 5545.756 kb on - strandat 5545.795 kb on + strandat 5545.859 kb on - strandat 5545.859 kb on - strandat 5546.102 kb on + strandat 5546.483 kb on - strandat 5546.592 kb on + strandat 5546.613 kb on + strandat 5546.661 kb on + strandat 5546.669 kb on - strandat 5546.746 kb on - strand, within BWI76_RS26770at 5546.757 kb on + strand, within BWI76_RS26770at 5546.802 kb on - strand, within BWI76_RS26770at 5546.802 kb on - strand, within BWI76_RS26770at 5546.802 kb on - strand, within BWI76_RS26770

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Per-strain Table

Position Strand Gene LocusTag Fraction A22 0.1 mg/ml
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5,543,274 + +3.6
5,543,321 - BWI76_RS26760 0.64 +0.1
5,543,402 - BWI76_RS26760 0.71 +0.3
5,543,446 + BWI76_RS26760 0.74 -0.7
5,543,513 + BWI76_RS26760 0.79 -0.3
5,543,516 + BWI76_RS26760 0.80 -0.2
5,543,554 - BWI76_RS26760 0.83 +0.6
5,543,616 + BWI76_RS26760 0.87 +0.3
5,543,648 + BWI76_RS26760 0.90 -0.9
5,543,672 - -0.6
5,543,738 - -0.2
5,543,753 + -0.1
5,543,851 - -0.2
5,544,009 - -0.5
5,544,114 + -0.8
5,544,122 - -0.3
5,544,230 - BWI76_RS26765 0.15 -0.5
5,544,235 - BWI76_RS26765 0.15 -0.7
5,544,240 - BWI76_RS26765 0.15 +0.9
5,544,614 - BWI76_RS26765 0.34 +0.5
5,544,636 - BWI76_RS26765 0.35 -0.3
5,544,735 + BWI76_RS26765 0.40 -0.4
5,544,743 - BWI76_RS26765 0.40 -1.3
5,544,801 + BWI76_RS26765 0.43 -1.0
5,544,879 - BWI76_RS26765 0.47 +1.4
5,544,881 - BWI76_RS26765 0.47 -0.4
5,545,043 + BWI76_RS26765 0.55 -2.2
5,545,096 - BWI76_RS26765 0.58 -1.0
5,545,171 + BWI76_RS26765 0.62 -1.3
5,545,223 - BWI76_RS26765 0.64 -0.5
5,545,562 - BWI76_RS26765 0.81 -0.7
5,545,675 + BWI76_RS26765 0.87 -0.7
5,545,735 - BWI76_RS26765 0.90 -2.0
5,545,735 - BWI76_RS26765 0.90 +1.0
5,545,756 - -0.9
5,545,795 + +2.0
5,545,859 - -0.6
5,545,859 - -0.2
5,546,102 + -1.1
5,546,483 - +0.4
5,546,592 + -0.4
5,546,613 + +1.2
5,546,661 + +0.3
5,546,669 - -0.8
5,546,746 - BWI76_RS26770 0.11 +0.4
5,546,757 + BWI76_RS26770 0.11 -0.3
5,546,802 - BWI76_RS26770 0.12 -0.5
5,546,802 - BWI76_RS26770 0.12 -0.6
5,546,802 - BWI76_RS26770 0.12 -1.1

Or see this region's nucleotide sequence