Experiment: A22 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26065 and BWI76_RS26070 overlap by 8 nucleotides BWI76_RS26070 and BWI76_RS26075 are separated by 17 nucleotides BWI76_RS26075 and BWI76_RS26080 are separated by 78 nucleotides
BWI76_RS26065: BWI76_RS26065 - phosphoglycolate phosphatase, at 5,386,437 to 5,387,186
_RS26065
BWI76_RS26070: BWI76_RS26070 - ribulose-phosphate 3-epimerase, at 5,387,179 to 5,387,856
_RS26070
BWI76_RS26075: BWI76_RS26075 - DNA adenine methylase, at 5,387,874 to 5,388,701
_RS26075
BWI76_RS26080: BWI76_RS26080 - cell division protein DamX, at 5,388,780 to 5,390,093
_RS26080
Position (kb) 5387
5388
5389 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5386.993 kb on + strand, within BWI76_RS26065 at 5387.143 kb on - strand at 5387.194 kb on - strand at 5387.903 kb on - strand at 5388.260 kb on + strand, within BWI76_RS26075 at 5388.314 kb on - strand, within BWI76_RS26075 at 5388.314 kb on - strand, within BWI76_RS26075 at 5388.432 kb on - strand, within BWI76_RS26075 at 5388.454 kb on + strand, within BWI76_RS26075 at 5388.513 kb on + strand, within BWI76_RS26075 at 5388.557 kb on - strand, within BWI76_RS26075 at 5388.805 kb on - strand at 5388.873 kb on - strand at 5388.908 kb on - strand at 5388.910 kb on + strand at 5388.910 kb on + strand at 5388.944 kb on - strand, within BWI76_RS26080 at 5388.997 kb on - strand, within BWI76_RS26080 at 5389.031 kb on - strand, within BWI76_RS26080 at 5389.035 kb on - strand, within BWI76_RS26080 at 5389.045 kb on - strand, within BWI76_RS26080 at 5389.045 kb on - strand, within BWI76_RS26080 at 5389.503 kb on + strand, within BWI76_RS26080 at 5389.535 kb on - strand, within BWI76_RS26080 at 5389.670 kb on + strand, within BWI76_RS26080 at 5389.675 kb on + strand, within BWI76_RS26080
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.1 mg/ml remove 5,386,993 + BWI76_RS26065 0.74 -0.3 5,387,143 - -0.3 5,387,194 - -0.1 5,387,903 - -1.5 5,388,260 + BWI76_RS26075 0.47 -1.5 5,388,314 - BWI76_RS26075 0.53 -1.9 5,388,314 - BWI76_RS26075 0.53 -1.8 5,388,432 - BWI76_RS26075 0.67 -2.8 5,388,454 + BWI76_RS26075 0.70 -1.4 5,388,513 + BWI76_RS26075 0.77 -2.4 5,388,557 - BWI76_RS26075 0.82 -0.7 5,388,805 - -0.1 5,388,873 - -0.6 5,388,908 - +0.7 5,388,910 + -1.1 5,388,910 + -3.1 5,388,944 - BWI76_RS26080 0.12 -1.5 5,388,997 - BWI76_RS26080 0.17 +0.9 5,389,031 - BWI76_RS26080 0.19 -0.7 5,389,035 - BWI76_RS26080 0.19 -0.2 5,389,045 - BWI76_RS26080 0.20 +0.6 5,389,045 - BWI76_RS26080 0.20 -0.8 5,389,503 + BWI76_RS26080 0.55 -2.6 5,389,535 - BWI76_RS26080 0.57 -0.9 5,389,670 + BWI76_RS26080 0.68 -2.5 5,389,675 + BWI76_RS26080 0.68 -2.7
Or see this region's nucleotide sequence